Square wave cathodic stripping voltammetric technique for determination of aflatoxin B1 in ground nut sample
An electroanalytical method has been developed for the detection and determination of 2,3,6a,9a-tetrahydro-4-methoxycyclo penta[c] furo[3’,2’:4,5] furo [2,3-h][l] benzopyran-1,11-dione (aflatoxin B1, AFB1) by a square wave cathodic stripping voltammetric (SWSV) technique on a hanging mercury drop el...
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| Format: | Article |
| Language: | English |
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The Malaysian Analytical Sciences Society
2008
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| Online Access: | http://eprints.utm.my/8583/ http://eprints.utm.my/8583/1/AbdullRahimMohd2008_SquareWaveCathodicStrippingVoltammetric.pdf |
| _version_ | 1848891722237476864 |
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| author | Yaacob, Mohamad Hadzi Mohd. Yusoff, Abd. Rahim Ahmad, Rahmalan |
| author_facet | Yaacob, Mohamad Hadzi Mohd. Yusoff, Abd. Rahim Ahmad, Rahmalan |
| author_sort | Yaacob, Mohamad Hadzi |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | An electroanalytical method has been developed for the detection and determination of 2,3,6a,9a-tetrahydro-4-methoxycyclo penta[c] furo[3’,2’:4,5] furo [2,3-h][l] benzopyran-1,11-dione (aflatoxin B1, AFB1) by a square wave cathodic stripping voltammetric (SWSV) technique on a hanging mercury drop electrode (HMDE) in aqueous solution with Britton-Robinson Buffer (BRB) at pH 9.0 as the supporting electrolyte. Effect of instrumental parameters such as accumulation potential (Eacc), accumulation time (tacc), scan rate (v), square wave frequency, step potential and pulse amplitude were examined. The best
condition were found to be Eacc of -0.8 V, tacc of 100 s, v of 3750 mVs-1, frequency of 125 Hz, voltage step of 30 mV and pulse amplitude of 50 mV. Calibration curve was linear in the range of 0.01 to 0.15 uM with a detection limit of 0.125 x 10-8 M. Relative standard deviation for a replicate measurement of AFB1 (n = 5) with a concentration of 0.01 ?M was 0.83% with a peak potential of -1.30 V (against Ag/AgCl). The recovery values obtained in spiked ground nut elute sample were 94.00 ± 0.67 % for 3.0 ppb, 91.22 ± 1.56 % for 9 ppb and 92.56 ± 2.00 % for 15.0 ppb of AFB1. The method was applied for the determination of the AFB1 in ground nut samples after extraction and clean-up steps. The results were compared with that obtained by high performance liquid chromatography (HPLC) technique.
Abstrak
Satu kaedah elektroanalisis telah dibangunkan untuk mengesan dan menentukan 2,3,6a,9a-tetrahydro-4-methoxycyclo
penta[c] furo[3’,2’:4,5] furo [2,3-h][l] benzopyran-1,11-dione (aflatoxin B1, AFB1) menggunakan teknik voltammetri
perlucutan katodik denyut pembeza di atas elektrod titisan raksa tergantung (HMDE) di dalam larutan akuas dengan larutan penimbal Britton-Robinson (BRB) pada pH 9.0 bertindak sebagai larutan penyokong. Kesan parameter peralatan seperti keupayaan pengumpulan (Eacc), masa pengumpulan (tacc), kadar imbasan (v), frekuensi gelombang bersegi, kenaikan keupayaan dan amplitud denyut telah dikaji. Keadaan terbaik yang diperolehi adalah Eacc; -0.8 V, tacc; 100 s, v; 3750 mV/s, frekuensi; 125 Hz, kenaikan keupayaan; 30 mV dan amplitud denyut; 50 mV. Keluk kalibrasi adalah linear pada julat diantara 0.01 ke 0.15 ?M dengan had pengesan pada 0.125 x 10-8 M. Sisihan piawai relatif untuk 5 kali pengukuran AFB1 dengan kepekatan 0.01uM ialah 0.83 %. Nilai perolehan semula di dalam larutan elusi sampel kacang yang disuntik dengan 3.0 ppb, 9 ppb dan 15.0 ppb AFB1 adalah 94.00 ± 0.67 %, 91.22 ± 1.56 % dan 92.56 ± 2.00 % masing-masingnya. Kaedah ini telah digunakan untuk menentukan kandungan AFB1 di dalam sampel kacang tanah selepas proses pengekstraksian dan
pembersihan dijalankan |
| first_indexed | 2025-11-15T21:02:29Z |
| format | Article |
| id | utm-8583 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T21:02:29Z |
| publishDate | 2008 |
| publisher | The Malaysian Analytical Sciences Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-85832012-11-19T06:25:05Z http://eprints.utm.my/8583/ Square wave cathodic stripping voltammetric technique for determination of aflatoxin B1 in ground nut sample Yaacob, Mohamad Hadzi Mohd. Yusoff, Abd. Rahim Ahmad, Rahmalan QC Physics An electroanalytical method has been developed for the detection and determination of 2,3,6a,9a-tetrahydro-4-methoxycyclo penta[c] furo[3’,2’:4,5] furo [2,3-h][l] benzopyran-1,11-dione (aflatoxin B1, AFB1) by a square wave cathodic stripping voltammetric (SWSV) technique on a hanging mercury drop electrode (HMDE) in aqueous solution with Britton-Robinson Buffer (BRB) at pH 9.0 as the supporting electrolyte. Effect of instrumental parameters such as accumulation potential (Eacc), accumulation time (tacc), scan rate (v), square wave frequency, step potential and pulse amplitude were examined. The best condition were found to be Eacc of -0.8 V, tacc of 100 s, v of 3750 mVs-1, frequency of 125 Hz, voltage step of 30 mV and pulse amplitude of 50 mV. Calibration curve was linear in the range of 0.01 to 0.15 uM with a detection limit of 0.125 x 10-8 M. Relative standard deviation for a replicate measurement of AFB1 (n = 5) with a concentration of 0.01 ?M was 0.83% with a peak potential of -1.30 V (against Ag/AgCl). The recovery values obtained in spiked ground nut elute sample were 94.00 ± 0.67 % for 3.0 ppb, 91.22 ± 1.56 % for 9 ppb and 92.56 ± 2.00 % for 15.0 ppb of AFB1. The method was applied for the determination of the AFB1 in ground nut samples after extraction and clean-up steps. The results were compared with that obtained by high performance liquid chromatography (HPLC) technique. Abstrak Satu kaedah elektroanalisis telah dibangunkan untuk mengesan dan menentukan 2,3,6a,9a-tetrahydro-4-methoxycyclo penta[c] furo[3’,2’:4,5] furo [2,3-h][l] benzopyran-1,11-dione (aflatoxin B1, AFB1) menggunakan teknik voltammetri perlucutan katodik denyut pembeza di atas elektrod titisan raksa tergantung (HMDE) di dalam larutan akuas dengan larutan penimbal Britton-Robinson (BRB) pada pH 9.0 bertindak sebagai larutan penyokong. Kesan parameter peralatan seperti keupayaan pengumpulan (Eacc), masa pengumpulan (tacc), kadar imbasan (v), frekuensi gelombang bersegi, kenaikan keupayaan dan amplitud denyut telah dikaji. Keadaan terbaik yang diperolehi adalah Eacc; -0.8 V, tacc; 100 s, v; 3750 mV/s, frekuensi; 125 Hz, kenaikan keupayaan; 30 mV dan amplitud denyut; 50 mV. Keluk kalibrasi adalah linear pada julat diantara 0.01 ke 0.15 ?M dengan had pengesan pada 0.125 x 10-8 M. Sisihan piawai relatif untuk 5 kali pengukuran AFB1 dengan kepekatan 0.01uM ialah 0.83 %. Nilai perolehan semula di dalam larutan elusi sampel kacang yang disuntik dengan 3.0 ppb, 9 ppb dan 15.0 ppb AFB1 adalah 94.00 ± 0.67 %, 91.22 ± 1.56 % dan 92.56 ± 2.00 % masing-masingnya. Kaedah ini telah digunakan untuk menentukan kandungan AFB1 di dalam sampel kacang tanah selepas proses pengekstraksian dan pembersihan dijalankan The Malaysian Analytical Sciences Society 2008 Article PeerReviewed application/pdf en http://eprints.utm.my/8583/1/AbdullRahimMohd2008_SquareWaveCathodicStrippingVoltammetric.pdf Yaacob, Mohamad Hadzi and Mohd. Yusoff, Abd. Rahim and Ahmad, Rahmalan (2008) Square wave cathodic stripping voltammetric technique for determination of aflatoxin B1 in ground nut sample. The Malaysian Journal of Analytical Sciences, 12 (1). pp. 132-141. ISSN 1394-2506 http://www.ukm.my/mjas/v12_n1/19.pdf |
| spellingShingle | QC Physics Yaacob, Mohamad Hadzi Mohd. Yusoff, Abd. Rahim Ahmad, Rahmalan Square wave cathodic stripping voltammetric technique for determination of aflatoxin B1 in ground nut sample |
| title | Square wave cathodic stripping voltammetric technique for determination of aflatoxin B1 in ground nut sample
|
| title_full | Square wave cathodic stripping voltammetric technique for determination of aflatoxin B1 in ground nut sample
|
| title_fullStr | Square wave cathodic stripping voltammetric technique for determination of aflatoxin B1 in ground nut sample
|
| title_full_unstemmed | Square wave cathodic stripping voltammetric technique for determination of aflatoxin B1 in ground nut sample
|
| title_short | Square wave cathodic stripping voltammetric technique for determination of aflatoxin B1 in ground nut sample
|
| title_sort | square wave cathodic stripping voltammetric technique for determination of aflatoxin b1 in ground nut sample |
| topic | QC Physics |
| url | http://eprints.utm.my/8583/ http://eprints.utm.my/8583/ http://eprints.utm.my/8583/1/AbdullRahimMohd2008_SquareWaveCathodicStrippingVoltammetric.pdf |