Polyhydroxyalkanoates (PHAs) production from aerobicmixed cultures

This study assessed the optimal conditions for PHA production using saponified fatty acid, derived from sunflower oil (SO). A fed-batch reactor was used to produce PHA. A mixture of sewage and semi-treated oil palm effluent from a facultative pond was cultured for six months to obtain a steady-stat...

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Main Authors: Md. Din, Mohd. Fadhli, Ujang, Zaini, Loosdrecht, Mark Van, Mohd. Yunus, Salmiati, Ahmad, Mohd. Azlan
Format: Article
Language:English
Published: Faculty of Civil Engineering, Universiti Teknologi Malaysia 2006
Subjects:
Online Access:http://eprints.utm.my/8407/
http://eprints.utm.my/8407/1/8407.pdf
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author Md. Din, Mohd. Fadhli
Ujang, Zaini
Loosdrecht, Mark Van
Mohd. Yunus, Salmiati
Ahmad, Mohd. Azlan
author_facet Md. Din, Mohd. Fadhli
Ujang, Zaini
Loosdrecht, Mark Van
Mohd. Yunus, Salmiati
Ahmad, Mohd. Azlan
author_sort Md. Din, Mohd. Fadhli
building UTeM Institutional Repository
collection Online Access
description This study assessed the optimal conditions for PHA production using saponified fatty acid, derived from sunflower oil (SO). A fed-batch reactor was used to produce PHA. A mixture of sewage and semi-treated oil palm effluent from a facultative pond was cultured for six months to obtain a steady-state condition. The culture was then transferred to the fed-batch system. SO mainly contains long-chain-fatty-acid (LCFA) with unsaturated fatty acid fractions of C14:1 – C18:3, therefore the PHA production was assessed under feast-famine condition. The main purpose of supplementing saponified SO was to improve the production of PHA constituents (copolymer of hydroxyl-unit) such as hydroxybutyrates (HBs), hydroxyvalerates (HVs) and hydroxyhexanoates (HHs) in mixed cultures. Fed-batch operation under aerobic-mixed cultures increased the PHA production up to 33% of the dried cell. Although sludge submitted to aerobic condition in mixed cultures could improve the PHA production, the production rates are still low. This study found that the HBs constituent in the sludge is always higher compared to HVs and HHs. Saponified SO has high specific PHA storage rates (qpfeast = 0.5 C-mol/C-mol. h) which are comparable to other vegetable oils (e.g. corn oil, soy bean oil, etc.)
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spelling utm-84072010-06-02T01:54:58Z http://eprints.utm.my/8407/ Polyhydroxyalkanoates (PHAs) production from aerobicmixed cultures Md. Din, Mohd. Fadhli Ujang, Zaini Loosdrecht, Mark Van Mohd. Yunus, Salmiati Ahmad, Mohd. Azlan TA Engineering (General). Civil engineering (General) This study assessed the optimal conditions for PHA production using saponified fatty acid, derived from sunflower oil (SO). A fed-batch reactor was used to produce PHA. A mixture of sewage and semi-treated oil palm effluent from a facultative pond was cultured for six months to obtain a steady-state condition. The culture was then transferred to the fed-batch system. SO mainly contains long-chain-fatty-acid (LCFA) with unsaturated fatty acid fractions of C14:1 – C18:3, therefore the PHA production was assessed under feast-famine condition. The main purpose of supplementing saponified SO was to improve the production of PHA constituents (copolymer of hydroxyl-unit) such as hydroxybutyrates (HBs), hydroxyvalerates (HVs) and hydroxyhexanoates (HHs) in mixed cultures. Fed-batch operation under aerobic-mixed cultures increased the PHA production up to 33% of the dried cell. Although sludge submitted to aerobic condition in mixed cultures could improve the PHA production, the production rates are still low. This study found that the HBs constituent in the sludge is always higher compared to HVs and HHs. Saponified SO has high specific PHA storage rates (qpfeast = 0.5 C-mol/C-mol. h) which are comparable to other vegetable oils (e.g. corn oil, soy bean oil, etc.) Faculty of Civil Engineering, Universiti Teknologi Malaysia 2006 Article PeerReviewed application/pdf en http://eprints.utm.my/8407/1/8407.pdf Md. Din, Mohd. Fadhli and Ujang, Zaini and Loosdrecht, Mark Van and Mohd. Yunus, Salmiati and Ahmad, Mohd. Azlan (2006) Polyhydroxyalkanoates (PHAs) production from aerobicmixed cultures. Malaysian Journal of Civil Engineering, 18 (2). pp. 109-128. ISSN 1823-7843 http://portal.psz.utm.my/psz/index.php?option=com_content&task=view&id=128&Itemid=305&PHPSESSID=2c3b333a6ad0213a14710cb6a410bb1b
spellingShingle TA Engineering (General). Civil engineering (General)
Md. Din, Mohd. Fadhli
Ujang, Zaini
Loosdrecht, Mark Van
Mohd. Yunus, Salmiati
Ahmad, Mohd. Azlan
Polyhydroxyalkanoates (PHAs) production from aerobicmixed cultures
title Polyhydroxyalkanoates (PHAs) production from aerobicmixed cultures
title_full Polyhydroxyalkanoates (PHAs) production from aerobicmixed cultures
title_fullStr Polyhydroxyalkanoates (PHAs) production from aerobicmixed cultures
title_full_unstemmed Polyhydroxyalkanoates (PHAs) production from aerobicmixed cultures
title_short Polyhydroxyalkanoates (PHAs) production from aerobicmixed cultures
title_sort polyhydroxyalkanoates (phas) production from aerobicmixed cultures
topic TA Engineering (General). Civil engineering (General)
url http://eprints.utm.my/8407/
http://eprints.utm.my/8407/
http://eprints.utm.my/8407/1/8407.pdf