Production of cyclodextrin glucanotransferase (CGTase) from Bacillus sp. TS1-1: process optimisation
The effect of pH and temperature were investigated for an optimized condition of cyclodextrin glucanotransferase (CGTase) production from Bacillus sp. TS1-1. The optimization process was analysed using Central Composite Experimental Design in Response Surface Methodology (RSM) by Design Expert Versi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Universiti Malaysia Sabah
2003
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| Online Access: | http://eprints.utm.my/7845/ http://eprints.utm.my/7845/1/AzmilHaizamAhmadTarmizi2003_ProductionOfCyclodextrinGlucanotransferase.pdf |
| _version_ | 1848891558368116736 |
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| author | Ahmad Tarmizi, Azmil Haizam Md. Illias, Rosli Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Abdul Rahman, Roshanida Abdul Aziz, Suriani |
| author_facet | Ahmad Tarmizi, Azmil Haizam Md. Illias, Rosli Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Abdul Rahman, Roshanida Abdul Aziz, Suriani |
| author_sort | Ahmad Tarmizi, Azmil Haizam |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | The effect of pH and temperature were investigated for an optimized condition of cyclodextrin glucanotransferase (CGTase) production from Bacillus sp. TS1-1. The optimization process was analysed using Central Composite Experimental Design in Response Surface Methodology (RSM) by Design Expert Version 6.0.4 (StatEase, USA). This design was employed to derive a statistical model for the effects of pH and temperature on the production of CGTase from Bacillus sp. TS1-1. The coefficient of determination, R2 was found to be 97.85%. The temperature of 29.59C and the pH of 10.32 had been found to be the optimum conditions for the maximum production of CGTase after 24 hr of incubation. In these conditions, the CGTase activity was attained to be 75.48 U/ml, which was close to the model predictions. |
| first_indexed | 2025-11-15T20:59:52Z |
| format | Article |
| id | utm-7845 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:59:52Z |
| publishDate | 2003 |
| publisher | Universiti Malaysia Sabah |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-78452010-06-02T01:48:47Z http://eprints.utm.my/7845/ Production of cyclodextrin glucanotransferase (CGTase) from Bacillus sp. TS1-1: process optimisation Ahmad Tarmizi, Azmil Haizam Md. Illias, Rosli Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Abdul Rahman, Roshanida Abdul Aziz, Suriani T Technology (General) The effect of pH and temperature were investigated for an optimized condition of cyclodextrin glucanotransferase (CGTase) production from Bacillus sp. TS1-1. The optimization process was analysed using Central Composite Experimental Design in Response Surface Methodology (RSM) by Design Expert Version 6.0.4 (StatEase, USA). This design was employed to derive a statistical model for the effects of pH and temperature on the production of CGTase from Bacillus sp. TS1-1. The coefficient of determination, R2 was found to be 97.85%. The temperature of 29.59C and the pH of 10.32 had been found to be the optimum conditions for the maximum production of CGTase after 24 hr of incubation. In these conditions, the CGTase activity was attained to be 75.48 U/ml, which was close to the model predictions. Universiti Malaysia Sabah 2003 Article PeerReviewed application/pdf en http://eprints.utm.my/7845/1/AzmilHaizamAhmadTarmizi2003_ProductionOfCyclodextrinGlucanotransferase.pdf Ahmad Tarmizi, Azmil Haizam and Md. Illias, Rosli and Md. Salleh, Madihah and Hassan, Osman and Kamaruddin, Kamarulzaman and Abdul Rahman, Roshanida and Abdul Aziz, Suriani (2003) Production of cyclodextrin glucanotransferase (CGTase) from Bacillus sp. TS1-1: process optimisation. Proceedings of International Conference On Chemical and Bioprocess Engineering . pp. 236-239. |
| spellingShingle | T Technology (General) Ahmad Tarmizi, Azmil Haizam Md. Illias, Rosli Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Abdul Rahman, Roshanida Abdul Aziz, Suriani Production of cyclodextrin glucanotransferase (CGTase) from Bacillus sp. TS1-1: process optimisation |
| title | Production of cyclodextrin glucanotransferase (CGTase) from Bacillus sp. TS1-1: process optimisation |
| title_full | Production of cyclodextrin glucanotransferase (CGTase) from Bacillus sp. TS1-1: process optimisation |
| title_fullStr | Production of cyclodextrin glucanotransferase (CGTase) from Bacillus sp. TS1-1: process optimisation |
| title_full_unstemmed | Production of cyclodextrin glucanotransferase (CGTase) from Bacillus sp. TS1-1: process optimisation |
| title_short | Production of cyclodextrin glucanotransferase (CGTase) from Bacillus sp. TS1-1: process optimisation |
| title_sort | production of cyclodextrin glucanotransferase (cgtase) from bacillus sp. ts1-1: process optimisation |
| topic | T Technology (General) |
| url | http://eprints.utm.my/7845/ http://eprints.utm.my/7845/1/AzmilHaizamAhmadTarmizi2003_ProductionOfCyclodextrinGlucanotransferase.pdf |