Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology
Cyclodextrin glucanotransferase (CGTase) activity was observed when the bacterium was grown in the medium at various initial pH values, containing carbon, nitrogen, phosphorus and mineral salt sources at 50 oC for 24 h in the shake flasks. The optimisation of this growth medium was carried out using...
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| Format: | Article |
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Elsevier Science B.V., Amsterdam
2004
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| Online Access: | http://eprints.utm.my/772/ |
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| author | Abd. Rahman, Roshanida Md. Illias, Rosli Mohd Ghazali, Mohd Nawawi Ismail, Ahmad Fauzi Hassan, Osman Kamaruddin, Kamarulzaman |
| author_facet | Abd. Rahman, Roshanida Md. Illias, Rosli Mohd Ghazali, Mohd Nawawi Ismail, Ahmad Fauzi Hassan, Osman Kamaruddin, Kamarulzaman |
| author_sort | Abd. Rahman, Roshanida |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | Cyclodextrin glucanotransferase (CGTase) activity was observed when the bacterium was grown in the medium at various initial pH values, containing carbon, nitrogen, phosphorus and mineral salt sources at 50 oC for 24 h in the shake flasks. The optimisation of this growth medium was carried out using response surface methodology. The design contains a total of 32 experimental trials involving 10 star points and 6 replicates at the centre points. The design was employed by selecting sago starch, peptone from casein, K2HPO4, CaCl2 and initial pH as five independent variables in this study. The optimal calculated values of tested variables for maximal production of CGTase were found to be comprised of: sago starch, 16.02 g/l; peptone from casein, 20 g/l; K2HPO4, 1.4 g/l; CaCl2, 0.2 g/l and initial pH, 7.54 with a predicted CGTase activity of 14.20 U/ml. These predicted optimal parameters were tested in the laboratory and the final CGTase activity obtained was very close to the predicted value at 14.80 U/ml. |
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| format | Article |
| id | utm-772 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T20:34:59Z |
| publishDate | 2004 |
| publisher | Elsevier Science B.V., Amsterdam |
| recordtype | eprints |
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| spelling | utm-7722017-03-06T08:08:03Z http://eprints.utm.my/772/ Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology Abd. Rahman, Roshanida Md. Illias, Rosli Mohd Ghazali, Mohd Nawawi Ismail, Ahmad Fauzi Hassan, Osman Kamaruddin, Kamarulzaman TP Chemical technology Cyclodextrin glucanotransferase (CGTase) activity was observed when the bacterium was grown in the medium at various initial pH values, containing carbon, nitrogen, phosphorus and mineral salt sources at 50 oC for 24 h in the shake flasks. The optimisation of this growth medium was carried out using response surface methodology. The design contains a total of 32 experimental trials involving 10 star points and 6 replicates at the centre points. The design was employed by selecting sago starch, peptone from casein, K2HPO4, CaCl2 and initial pH as five independent variables in this study. The optimal calculated values of tested variables for maximal production of CGTase were found to be comprised of: sago starch, 16.02 g/l; peptone from casein, 20 g/l; K2HPO4, 1.4 g/l; CaCl2, 0.2 g/l and initial pH, 7.54 with a predicted CGTase activity of 14.20 U/ml. These predicted optimal parameters were tested in the laboratory and the final CGTase activity obtained was very close to the predicted value at 14.80 U/ml. Elsevier Science B.V., Amsterdam 2004 Article PeerReviewed Abd. Rahman, Roshanida and Md. Illias, Rosli and Mohd Ghazali, Mohd Nawawi and Ismail, Ahmad Fauzi and Hassan, Osman and Kamaruddin, Kamarulzaman (2004) Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology. Process Biochemistry, 39 . pp. 2053-2060. ISSN 0032-9592 http://dx.doi.org/10.1016/j.procbio.2003.10.014 10.1016/j.procbio.2003.10.014 |
| spellingShingle | TP Chemical technology Abd. Rahman, Roshanida Md. Illias, Rosli Mohd Ghazali, Mohd Nawawi Ismail, Ahmad Fauzi Hassan, Osman Kamaruddin, Kamarulzaman Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology |
| title | Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology |
| title_full | Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology |
| title_fullStr | Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology |
| title_full_unstemmed | Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology |
| title_short | Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology |
| title_sort | optimisation of growth medium for the production of cyclodextrin glucanotransferase from bacillus stearothermophilus hr1 using response surface methodology |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/772/ http://eprints.utm.my/772/ http://eprints.utm.my/772/ |