Insulin potentiating activity of cinnamomum extract on 3T3-L1 adipocytes

Non-insulin dependent diabetes melitus (NIDDM), or Type II diabetes is one of the largest health problems in the world. Several plant phytochemicals can directly stimulate insulin secretion and/or action. In this work, the activity of chalcone polymer isolated from Cinnamomum zeylanicum on 3T3-L1 ad...

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Main Authors: M., Taher, Abdul Majid, Fadzilah Adibah, Sarmidi, Mohamad Roji
Format: Article
Language:English
Published: Universiti Malaysia Sabah 2003
Subjects:
Online Access:http://eprints.utm.my/6946/
http://eprints.utm.my/6946/1/M.Taher2003_InsulinPotentiatingActivityOfCinnamomumExtract.pdf
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author M., Taher
Abdul Majid, Fadzilah Adibah
Sarmidi, Mohamad Roji
author_facet M., Taher
Abdul Majid, Fadzilah Adibah
Sarmidi, Mohamad Roji
author_sort M., Taher
building UTeM Institutional Repository
collection Online Access
description Non-insulin dependent diabetes melitus (NIDDM), or Type II diabetes is one of the largest health problems in the world. Several plant phytochemicals can directly stimulate insulin secretion and/or action. In this work, the activity of chalcone polymer isolated from Cinnamomum zeylanicum on 3T3-L1 adipocytes was investigated. The compound was isolated using silica gel column chromatography. The result shows that at a dose of 100μg/mL, the compound gave the higher action than insulin alone by increasing glucose consumption 3 hours after simulation. When the compound was combined with insulin (100nM), the glucose consumption increased about 25%. In contrast when the compound was administered on preconfluent 3T3-L1 preadipocytes, there was no significant effect. Cell proliferation also increased after addition of the compound. Cell proliferation was detected by MTT assay. Based on the experimental data it can be shown that the compound promote glucose metabolism and subsequently enhanced cell growth. The type of action of the compound was synergic effect to insulin activity.
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spelling utm-69462010-06-01T15:48:33Z http://eprints.utm.my/6946/ Insulin potentiating activity of cinnamomum extract on 3T3-L1 adipocytes M., Taher Abdul Majid, Fadzilah Adibah Sarmidi, Mohamad Roji T Technology (General) Non-insulin dependent diabetes melitus (NIDDM), or Type II diabetes is one of the largest health problems in the world. Several plant phytochemicals can directly stimulate insulin secretion and/or action. In this work, the activity of chalcone polymer isolated from Cinnamomum zeylanicum on 3T3-L1 adipocytes was investigated. The compound was isolated using silica gel column chromatography. The result shows that at a dose of 100μg/mL, the compound gave the higher action than insulin alone by increasing glucose consumption 3 hours after simulation. When the compound was combined with insulin (100nM), the glucose consumption increased about 25%. In contrast when the compound was administered on preconfluent 3T3-L1 preadipocytes, there was no significant effect. Cell proliferation also increased after addition of the compound. Cell proliferation was detected by MTT assay. Based on the experimental data it can be shown that the compound promote glucose metabolism and subsequently enhanced cell growth. The type of action of the compound was synergic effect to insulin activity. Universiti Malaysia Sabah 2003 Article PeerReviewed application/pdf en http://eprints.utm.my/6946/1/M.Taher2003_InsulinPotentiatingActivityOfCinnamomumExtract.pdf M., Taher and Abdul Majid, Fadzilah Adibah and Sarmidi, Mohamad Roji (2003) Insulin potentiating activity of cinnamomum extract on 3T3-L1 adipocytes. Proceedings of International Conference On Chemical and Bioprocess Engineering . pp. 270-273.
spellingShingle T Technology (General)
M., Taher
Abdul Majid, Fadzilah Adibah
Sarmidi, Mohamad Roji
Insulin potentiating activity of cinnamomum extract on 3T3-L1 adipocytes
title Insulin potentiating activity of cinnamomum extract on 3T3-L1 adipocytes
title_full Insulin potentiating activity of cinnamomum extract on 3T3-L1 adipocytes
title_fullStr Insulin potentiating activity of cinnamomum extract on 3T3-L1 adipocytes
title_full_unstemmed Insulin potentiating activity of cinnamomum extract on 3T3-L1 adipocytes
title_short Insulin potentiating activity of cinnamomum extract on 3T3-L1 adipocytes
title_sort insulin potentiating activity of cinnamomum extract on 3t3-l1 adipocytes
topic T Technology (General)
url http://eprints.utm.my/6946/
http://eprints.utm.my/6946/1/M.Taher2003_InsulinPotentiatingActivityOfCinnamomumExtract.pdf