Cloning and expression of 3-hydroxybutyrate dehydrogenase gene from locally isolated Pseudomonas sp.
The 3-HBDH gene from Pseudomonas sp. was amplified, cloned, and sequenced. The deduced amino acid sequence was highly matched with 3-HBDH sequences in DDBJ/GenBank/EMBL. But Pseudomonas sp. 3-HBDH was lack of the C-terminal region found in mammalian enzymes containing a lipid-binding domain that is...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universiti Malaysia Sabah
2003
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| Subjects: | |
| Online Access: | http://eprints.utm.my/6922/ http://eprints.utm.my/6922/1/NikAzmiNikMahmod2003_CloningAndExpressionOf3-hydroxybutyrateDehydrogenase.pdf |
| Summary: | The 3-HBDH gene from Pseudomonas sp. was amplified, cloned, and sequenced. The deduced amino acid sequence was highly matched with 3-HBDH sequences in DDBJ/GenBank/EMBL. But Pseudomonas sp. 3-HBDH was lack of the C-terminal region found in mammalian enzymes containing a lipid-binding domain that is important for the 3-HBDH's activity. The E-coli XL1 blue cells transformed with the resultant plasmid, pBDH-1 showed 3-HBDH's activity. The E. coli XL1 Blue cells transformed with resultant plasmid, pBDH-1 showed 3-HBDH's activity. The nucleotide sequence of recombinant pBDH-1 indicate functional oligomers composed of subunits of 225 amino acid with a calculated Mr of 26, 855 Da and proved on SDS-PAGE. Ammonium sulfate farctionation resulted in low yield of the enzyme but its activity was comparably high with other 3-HBDHs. The recombinant enzyme showed a broad range of stability with respect to pH and temperature. |
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