Polyhydroxyalkanoates (PHAs) production from saponified sunflower oil in mixed cultures under aerobic condition

A laboratory study was conducted to assess the optimal conditions for polyhyroxyalkanoates (PHA) production using saponified sunflower oil (SO). The system was operated under continuous sequential phase of growth and accumulation conditions. The main purpose of using saponified SO was to improve the...

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Main Authors: Mohd. Fadhil, Mohd. Fadhil, Ujang, Zaini, Van Loosdrecht, Mark, Ahmad, Mohd. Azlan
Format: Article
Language:English
Published: Penerbit UTM Press 2008
Subjects:
Online Access:http://eprints.utm.my/6714/
http://eprints.utm.my/6714/10/MohdFadhilMohdFadhil2008_Polyhydroxyalkanoates%28PHAs%29ProductionfromSaponifiedSunflower.pdf
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author Mohd. Fadhil, Mohd. Fadhil
Ujang, Zaini
Van Loosdrecht, Mark
Ahmad, Mohd. Azlan
author_facet Mohd. Fadhil, Mohd. Fadhil
Ujang, Zaini
Van Loosdrecht, Mark
Ahmad, Mohd. Azlan
author_sort Mohd. Fadhil, Mohd. Fadhil
building UTeM Institutional Repository
collection Online Access
description A laboratory study was conducted to assess the optimal conditions for polyhyroxyalkanoates (PHA) production using saponified sunflower oil (SO). The system was operated under continuous sequential phase of growth and accumulation conditions. The main purpose of using saponified SO was to improve the quality of PHA constituents (e.g. copolymer of hydroxyl-unit) such as hydroxybutyrates (HBs), hydroxyvalerates (HVs) and hydroxyhexanoates (HHs). Typically, SO mainly contains long-chain-fatty-acid (LCFA) with unsaturated fatty acid (C14:1 – C18:3) and therefore, the selected PHA-producer would be developed during unbalanced growth (feast-famine condition). Operation of the fed-batch for almost 6 months under aerobic dynamic conditions (with various operating conditions) has increased the PHA production up to 33% of dried cell weight. It was also shown that sludge subjected to aerobic condition in mixed cultures could accumulate high amount of PHA by manipulating the cycle length (HRT study). The high specific PHA storage rate (qpfeast = 0.5 C-mol/C-mol. h) as well as the high sludge PHA contents achieved by mixed cultures make this saponified SO competitive with those based on the other vegetable oil (e.g. corn oil, soy oil, etc.)
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spelling utm-67142017-02-15T07:35:28Z http://eprints.utm.my/6714/ Polyhydroxyalkanoates (PHAs) production from saponified sunflower oil in mixed cultures under aerobic condition Mohd. Fadhil, Mohd. Fadhil Ujang, Zaini Van Loosdrecht, Mark Ahmad, Mohd. Azlan QH301 Biology A laboratory study was conducted to assess the optimal conditions for polyhyroxyalkanoates (PHA) production using saponified sunflower oil (SO). The system was operated under continuous sequential phase of growth and accumulation conditions. The main purpose of using saponified SO was to improve the quality of PHA constituents (e.g. copolymer of hydroxyl-unit) such as hydroxybutyrates (HBs), hydroxyvalerates (HVs) and hydroxyhexanoates (HHs). Typically, SO mainly contains long-chain-fatty-acid (LCFA) with unsaturated fatty acid (C14:1 – C18:3) and therefore, the selected PHA-producer would be developed during unbalanced growth (feast-famine condition). Operation of the fed-batch for almost 6 months under aerobic dynamic conditions (with various operating conditions) has increased the PHA production up to 33% of dried cell weight. It was also shown that sludge subjected to aerobic condition in mixed cultures could accumulate high amount of PHA by manipulating the cycle length (HRT study). The high specific PHA storage rate (qpfeast = 0.5 C-mol/C-mol. h) as well as the high sludge PHA contents achieved by mixed cultures make this saponified SO competitive with those based on the other vegetable oil (e.g. corn oil, soy oil, etc.) Penerbit UTM Press 2008-06 Article PeerReviewed application/pdf en http://eprints.utm.my/6714/10/MohdFadhilMohdFadhil2008_Polyhydroxyalkanoates%28PHAs%29ProductionfromSaponifiedSunflower.pdf Mohd. Fadhil, Mohd. Fadhil and Ujang, Zaini and Van Loosdrecht, Mark and Ahmad, Mohd. Azlan (2008) Polyhydroxyalkanoates (PHAs) production from saponified sunflower oil in mixed cultures under aerobic condition. Jurnal Teknologi (48F). pp. 1-19. ISSN 0127-9696 http://www.penerbit.utm.my/onlinejournal/48/F/JT48F1.pdf
spellingShingle QH301 Biology
Mohd. Fadhil, Mohd. Fadhil
Ujang, Zaini
Van Loosdrecht, Mark
Ahmad, Mohd. Azlan
Polyhydroxyalkanoates (PHAs) production from saponified sunflower oil in mixed cultures under aerobic condition
title Polyhydroxyalkanoates (PHAs) production from saponified sunflower oil in mixed cultures under aerobic condition
title_full Polyhydroxyalkanoates (PHAs) production from saponified sunflower oil in mixed cultures under aerobic condition
title_fullStr Polyhydroxyalkanoates (PHAs) production from saponified sunflower oil in mixed cultures under aerobic condition
title_full_unstemmed Polyhydroxyalkanoates (PHAs) production from saponified sunflower oil in mixed cultures under aerobic condition
title_short Polyhydroxyalkanoates (PHAs) production from saponified sunflower oil in mixed cultures under aerobic condition
title_sort polyhydroxyalkanoates (phas) production from saponified sunflower oil in mixed cultures under aerobic condition
topic QH301 Biology
url http://eprints.utm.my/6714/
http://eprints.utm.my/6714/
http://eprints.utm.my/6714/10/MohdFadhilMohdFadhil2008_Polyhydroxyalkanoates%28PHAs%29ProductionfromSaponifiedSunflower.pdf