Determination of effective diffusion coefficient of immobilized Baker's yeast invertase in various concentration of PVA-alginate matrix

Baker's yeast invertase is immobilized in PVA-alginate matrix using an improved method. PVA beads were prepared by adding calcium alginate to improve its stability, mechanical and chemical properties. Boric acid was used as the cross-linking agent and additional chemicals consisting of 10% bori...

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Main Authors: Mohd. Zain, N. A., Suhaimi, M. S., Idris, Ani
Format: Article
Language:English
Published: Faculty of Engineering, International Islamic University Malaysia 2007
Subjects:
Online Access:http://eprints.utm.my/5736/
http://eprints.utm.my/5736/1/MohdZainN.A.2007_DeterminationOfEffectiveDiffusionCoefficient.pdf
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author Mohd. Zain, N. A.
Suhaimi, M. S.
Idris, Ani
author_facet Mohd. Zain, N. A.
Suhaimi, M. S.
Idris, Ani
author_sort Mohd. Zain, N. A.
building UTeM Institutional Repository
collection Online Access
description Baker's yeast invertase is immobilized in PVA-alginate matrix using an improved method. PVA beads were prepared by adding calcium alginate to improve its stability, mechanical and chemical properties. Boric acid was used as the cross-linking agent and additional chemicals consisting of 10% boric acid and sodium sulphate solution was used as a treatment solution to harden the PVA-alginate beads. The determination of the effective diffusion of PVA-alginate matrix the vital step in optimizing the preparation of immobilized and water-soluble biocatalyst. In this study the two-level full factorial design was used to investigate the effect of PVA and boric acid concentrations and diffusions coefficient. Diffusion coefficient (De) is one of the factors that significantly affect the mass transport within the immobilization matrix. De value varies for each concentration of PVA and boric acid. The result concluded that both factors significantly affect the De. A maximum De value of 5.0141 x 10(-5) cm2s-1 was obtained at boric acid and PVA concentraion of 7w/v and 10.5 w/v respectively.
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spelling utm-57362017-09-12T04:29:43Z http://eprints.utm.my/5736/ Determination of effective diffusion coefficient of immobilized Baker's yeast invertase in various concentration of PVA-alginate matrix Mohd. Zain, N. A. Suhaimi, M. S. Idris, Ani T Technology (General) Baker's yeast invertase is immobilized in PVA-alginate matrix using an improved method. PVA beads were prepared by adding calcium alginate to improve its stability, mechanical and chemical properties. Boric acid was used as the cross-linking agent and additional chemicals consisting of 10% boric acid and sodium sulphate solution was used as a treatment solution to harden the PVA-alginate beads. The determination of the effective diffusion of PVA-alginate matrix the vital step in optimizing the preparation of immobilized and water-soluble biocatalyst. In this study the two-level full factorial design was used to investigate the effect of PVA and boric acid concentrations and diffusions coefficient. Diffusion coefficient (De) is one of the factors that significantly affect the mass transport within the immobilization matrix. De value varies for each concentration of PVA and boric acid. The result concluded that both factors significantly affect the De. A maximum De value of 5.0141 x 10(-5) cm2s-1 was obtained at boric acid and PVA concentraion of 7w/v and 10.5 w/v respectively. Faculty of Engineering, International Islamic University Malaysia 2007 Article PeerReviewed application/pdf en http://eprints.utm.my/5736/1/MohdZainN.A.2007_DeterminationOfEffectiveDiffusionCoefficient.pdf Mohd. Zain, N. A. and Suhaimi, M. S. and Idris, Ani (2007) Determination of effective diffusion coefficient of immobilized Baker's yeast invertase in various concentration of PVA-alginate matrix. Proceedings of the International Conference on Biotechnology Engineering ICBioE'07 . pp. 248-255.
spellingShingle T Technology (General)
Mohd. Zain, N. A.
Suhaimi, M. S.
Idris, Ani
Determination of effective diffusion coefficient of immobilized Baker's yeast invertase in various concentration of PVA-alginate matrix
title Determination of effective diffusion coefficient of immobilized Baker's yeast invertase in various concentration of PVA-alginate matrix
title_full Determination of effective diffusion coefficient of immobilized Baker's yeast invertase in various concentration of PVA-alginate matrix
title_fullStr Determination of effective diffusion coefficient of immobilized Baker's yeast invertase in various concentration of PVA-alginate matrix
title_full_unstemmed Determination of effective diffusion coefficient of immobilized Baker's yeast invertase in various concentration of PVA-alginate matrix
title_short Determination of effective diffusion coefficient of immobilized Baker's yeast invertase in various concentration of PVA-alginate matrix
title_sort determination of effective diffusion coefficient of immobilized baker's yeast invertase in various concentration of pva-alginate matrix
topic T Technology (General)
url http://eprints.utm.my/5736/
http://eprints.utm.my/5736/1/MohdZainN.A.2007_DeterminationOfEffectiveDiffusionCoefficient.pdf