Comparison of Signal-to-noise, Blank Determination, and Linear Regression Methods for the Estimation of Detection and Quantification Limits for Volatile Organic Compounds by Gas Chromatography
Limit of detection (LOD) and limit of quantification (LOQ) are two important performance characteristics in method validation. This work compares three methods based on the International Conference of Harmonization (ICH) and EURACHEM guidelines, namely, signal-to-noise, blank determination, and line...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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2008
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| Online Access: | http://eprints.utm.my/5670/ http://eprints.utm.my/5670/2/MarsinSanagi20080604_Comparison_of_SNR_Blank_Regression.pdf |
| _version_ | 1848891098123993088 |
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| author | Sanagi, Mohd Marsin Ling, Susie L. Nasir, Zalilah Hermawan, D. Wan Ibrahim, Wan Aini Abu Naim, Ahmedy |
| author_facet | Sanagi, Mohd Marsin Ling, Susie L. Nasir, Zalilah Hermawan, D. Wan Ibrahim, Wan Aini Abu Naim, Ahmedy |
| author_sort | Sanagi, Mohd Marsin |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | Limit of detection (LOD) and limit of quantification (LOQ) are two important performance characteristics in method validation. This work compares three methods based on the International Conference of Harmonization (ICH) and EURACHEM guidelines, namely, signal-to-noise, blank determination, and linear regression, to estimate the LOD and LOQ for volatile organic compounds (VOCs) by experimental methodology using gas chromatography. Five VOCs, i.e. toluene, ethylbenzene, iso-propylbenzene, n-propylbenzene and styrene, were chosen for the experimental study. The results indicated that the estimated LODs and LOQs were not equivalent and could vary by a factor of 5 to 6 for the different methods. It is therefore essential to have a clearly described procedure for estimating the LOD and LOQ during method validation to allow interlaboratory comparisons. |
| first_indexed | 2025-11-15T20:52:34Z |
| format | Article |
| id | utm-5670 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:52:34Z |
| publishDate | 2008 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-56702017-09-12T07:23:50Z http://eprints.utm.my/5670/ Comparison of Signal-to-noise, Blank Determination, and Linear Regression Methods for the Estimation of Detection and Quantification Limits for Volatile Organic Compounds by Gas Chromatography Sanagi, Mohd Marsin Ling, Susie L. Nasir, Zalilah Hermawan, D. Wan Ibrahim, Wan Aini Abu Naim, Ahmedy QD Chemistry Limit of detection (LOD) and limit of quantification (LOQ) are two important performance characteristics in method validation. This work compares three methods based on the International Conference of Harmonization (ICH) and EURACHEM guidelines, namely, signal-to-noise, blank determination, and linear regression, to estimate the LOD and LOQ for volatile organic compounds (VOCs) by experimental methodology using gas chromatography. Five VOCs, i.e. toluene, ethylbenzene, iso-propylbenzene, n-propylbenzene and styrene, were chosen for the experimental study. The results indicated that the estimated LODs and LOQs were not equivalent and could vary by a factor of 5 to 6 for the different methods. It is therefore essential to have a clearly described procedure for estimating the LOD and LOQ during method validation to allow interlaboratory comparisons. 2008 Article NonPeerReviewed application/pdf en http://eprints.utm.my/5670/2/MarsinSanagi20080604_Comparison_of_SNR_Blank_Regression.pdf Sanagi, Mohd Marsin and Ling, Susie L. and Nasir, Zalilah and Hermawan, D. and Wan Ibrahim, Wan Aini and Abu Naim, Ahmedy (2008) Comparison of Signal-to-noise, Blank Determination, and Linear Regression Methods for the Estimation of Detection and Quantification Limits for Volatile Organic Compounds by Gas Chromatography. Analytica Chimica Acta . (Unpublished) |
| spellingShingle | QD Chemistry Sanagi, Mohd Marsin Ling, Susie L. Nasir, Zalilah Hermawan, D. Wan Ibrahim, Wan Aini Abu Naim, Ahmedy Comparison of Signal-to-noise, Blank Determination, and Linear Regression Methods for the Estimation of Detection and Quantification Limits for Volatile Organic Compounds by Gas Chromatography |
| title | Comparison of Signal-to-noise, Blank Determination, and Linear Regression Methods for the Estimation of Detection and Quantification Limits for Volatile Organic Compounds by Gas Chromatography |
| title_full | Comparison of Signal-to-noise, Blank Determination, and Linear Regression Methods for the Estimation of Detection and Quantification Limits for Volatile Organic Compounds by Gas Chromatography |
| title_fullStr | Comparison of Signal-to-noise, Blank Determination, and Linear Regression Methods for the Estimation of Detection and Quantification Limits for Volatile Organic Compounds by Gas Chromatography |
| title_full_unstemmed | Comparison of Signal-to-noise, Blank Determination, and Linear Regression Methods for the Estimation of Detection and Quantification Limits for Volatile Organic Compounds by Gas Chromatography |
| title_short | Comparison of Signal-to-noise, Blank Determination, and Linear Regression Methods for the Estimation of Detection and Quantification Limits for Volatile Organic Compounds by Gas Chromatography |
| title_sort | comparison of signal-to-noise, blank determination, and linear regression methods for the estimation of detection and quantification limits for volatile organic compounds by gas chromatography |
| topic | QD Chemistry |
| url | http://eprints.utm.my/5670/ http://eprints.utm.my/5670/2/MarsinSanagi20080604_Comparison_of_SNR_Blank_Regression.pdf |