Optimization of CE separation of roxarsone and several arsenic compounds

Elektroforesis rerambut dengan pengesanan ultralembayung digunakan bagi pemisahan arsenit As(III), arsenat As(V), asid monometilarsonik (MMA), asid dimetilarsinik (DMA), asid fenilarsonik (PAA), asid ρ–arsanilik (ρ–ASA) dan roksarson (3–NHPAA). Pemisahan tercapai menggunakan kapilari silika terlakur...

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Main Authors: Jaafar, Jafariah, Ahamad, Rahmalan, Nasrullah, Nasrullah, Irwan, Zildawarni
Format: Article
Language:English
Published: Penerbit UTM Press 2006
Subjects:
Online Access:http://eprints.utm.my/5460/
http://eprints.utm.my/5460/1/JTJUN44C8.pdf
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author Jaafar, Jafariah
Ahamad, Rahmalan
Nasrullah, Nasrullah
Irwan, Zildawarni
author_facet Jaafar, Jafariah
Ahamad, Rahmalan
Nasrullah, Nasrullah
Irwan, Zildawarni
author_sort Jaafar, Jafariah
building UTeM Institutional Repository
collection Online Access
description Elektroforesis rerambut dengan pengesanan ultralembayung digunakan bagi pemisahan arsenit As(III), arsenat As(V), asid monometilarsonik (MMA), asid dimetilarsinik (DMA), asid fenilarsonik (PAA), asid ρ–arsanilik (ρ–ASA) dan roksarson (3–NHPAA). Pemisahan tercapai menggunakan kapilari silika terlakur dengan sel pengesan berkepekaan tinggi dan pengesan susun atur diod. Larutan penimbal 15 mM fosfat digunakan sebagai elektrolit latarbelakang. Pengaruh pH elektrolit, keupayaan yang dikenakan dan panjang gelombang pengesanan dikaji dengan nilai optimum diperoleh pada pH 6.0, keupayaan 25 kV dan panjang gelombang pengesanan pada 191 nm. Kaedah yang telah dioptimum ini memberi had pengesanan 0.193 mg/L bagi As(III). Kebolehulangan bagi semua analit adalah di antara julat 4.6%–10.5% RSD. Capillary electrophoresis with direct UV detection was used for the separation of arsenite As(III), arsenate As(V), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), phenylarsonic acid (PAA), ρ–arsanilic acid (ρ–ASA) and roxarsone (3–NHPAA). The separation was achieved in a fused silica capillary using a high sensitivity detection cell with diode array detector. A 15 mM phosphate buffer was used as the background electrolyte. The influence of electrolyte pH, applied voltage and wavelength were investigated in this research where the optimum conditions obtained were at pH 6.0, 25 kV voltage and 191 nm detection wavelength. The optimized method provided a limit of detection of 0.193 mg/L for As(III). Reproducibility of the analytes was in the range of 4.6%–10.5% RSD.
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spelling utm-54602017-11-01T04:17:29Z http://eprints.utm.my/5460/ Optimization of CE separation of roxarsone and several arsenic compounds Jaafar, Jafariah Ahamad, Rahmalan Nasrullah, Nasrullah Irwan, Zildawarni QD Chemistry Elektroforesis rerambut dengan pengesanan ultralembayung digunakan bagi pemisahan arsenit As(III), arsenat As(V), asid monometilarsonik (MMA), asid dimetilarsinik (DMA), asid fenilarsonik (PAA), asid ρ–arsanilik (ρ–ASA) dan roksarson (3–NHPAA). Pemisahan tercapai menggunakan kapilari silika terlakur dengan sel pengesan berkepekaan tinggi dan pengesan susun atur diod. Larutan penimbal 15 mM fosfat digunakan sebagai elektrolit latarbelakang. Pengaruh pH elektrolit, keupayaan yang dikenakan dan panjang gelombang pengesanan dikaji dengan nilai optimum diperoleh pada pH 6.0, keupayaan 25 kV dan panjang gelombang pengesanan pada 191 nm. Kaedah yang telah dioptimum ini memberi had pengesanan 0.193 mg/L bagi As(III). Kebolehulangan bagi semua analit adalah di antara julat 4.6%–10.5% RSD. Capillary electrophoresis with direct UV detection was used for the separation of arsenite As(III), arsenate As(V), monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), phenylarsonic acid (PAA), ρ–arsanilic acid (ρ–ASA) and roxarsone (3–NHPAA). The separation was achieved in a fused silica capillary using a high sensitivity detection cell with diode array detector. A 15 mM phosphate buffer was used as the background electrolyte. The influence of electrolyte pH, applied voltage and wavelength were investigated in this research where the optimum conditions obtained were at pH 6.0, 25 kV voltage and 191 nm detection wavelength. The optimized method provided a limit of detection of 0.193 mg/L for As(III). Reproducibility of the analytes was in the range of 4.6%–10.5% RSD. Penerbit UTM Press 2006-06 Article PeerReviewed application/pdf en http://eprints.utm.my/5460/1/JTJUN44C8.pdf Jaafar, Jafariah and Ahamad, Rahmalan and Nasrullah, Nasrullah and Irwan, Zildawarni (2006) Optimization of CE separation of roxarsone and several arsenic compounds. Jurnal Teknologi C (44C). pp. 77-88. ISSN 0127-9696 http://www.penerbit.utm.my/onlinejournal/44/C/JTJUN44C8.pdf
spellingShingle QD Chemistry
Jaafar, Jafariah
Ahamad, Rahmalan
Nasrullah, Nasrullah
Irwan, Zildawarni
Optimization of CE separation of roxarsone and several arsenic compounds
title Optimization of CE separation of roxarsone and several arsenic compounds
title_full Optimization of CE separation of roxarsone and several arsenic compounds
title_fullStr Optimization of CE separation of roxarsone and several arsenic compounds
title_full_unstemmed Optimization of CE separation of roxarsone and several arsenic compounds
title_short Optimization of CE separation of roxarsone and several arsenic compounds
title_sort optimization of ce separation of roxarsone and several arsenic compounds
topic QD Chemistry
url http://eprints.utm.my/5460/
http://eprints.utm.my/5460/
http://eprints.utm.my/5460/1/JTJUN44C8.pdf