Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus)
Bromelain is a complex of proteolytic enzymes obtained from pineapple (Ananas comosus) fruits and stem. These proteolytic enzymes are tools that promise an impressive number of medical and therapeutic uses, particularly as anti-inflammatory agents, dietary supplements and analgesics. The purpose of...
| Main Authors: | , , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2005
|
| Subjects: | |
| Online Access: | http://eprints.utm.my/5270/ http://eprints.utm.my/5270/1/MelissaLohPeiShian2005_PilotScaleExtractionOfProteolyticEnzyme.pdf |
| _version_ | 1848891008636420096 |
|---|---|
| author | Loh, Melissa Pei Shian Syamsul Baharil, Nur Aishah Ting, Lee Yu M., Taher Abdul Majid, Fadzilah Adibah |
| author_facet | Loh, Melissa Pei Shian Syamsul Baharil, Nur Aishah Ting, Lee Yu M., Taher Abdul Majid, Fadzilah Adibah |
| author_sort | Loh, Melissa Pei Shian |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | Bromelain is a complex of proteolytic enzymes obtained from pineapple (Ananas comosus) fruits and stem. These proteolytic enzymes are tools that promise an impressive number of medical and therapeutic uses, particularly as anti-inflammatory agents, dietary supplements and analgesics. The purpose of this study was to develop a pilot scale bromelain extraction process. The bromelain extraction method using pineapple fruit core in this study has yielded a total protein content of 9.33 mg/ml with the proteolytic activity of 1400 GDU/ml. An increase in homogenization cycle to increase enzyme yield has proven to be successful. Triple-loop homogenization has shown three times higher proteolytic activity than non-homogenized sample. |
| first_indexed | 2025-11-15T20:51:08Z |
| format | Conference or Workshop Item |
| id | utm-5270 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:51:08Z |
| publishDate | 2005 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-52702017-08-30T04:30:56Z http://eprints.utm.my/5270/ Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus) Loh, Melissa Pei Shian Syamsul Baharil, Nur Aishah Ting, Lee Yu M., Taher Abdul Majid, Fadzilah Adibah T Technology (General) Bromelain is a complex of proteolytic enzymes obtained from pineapple (Ananas comosus) fruits and stem. These proteolytic enzymes are tools that promise an impressive number of medical and therapeutic uses, particularly as anti-inflammatory agents, dietary supplements and analgesics. The purpose of this study was to develop a pilot scale bromelain extraction process. The bromelain extraction method using pineapple fruit core in this study has yielded a total protein content of 9.33 mg/ml with the proteolytic activity of 1400 GDU/ml. An increase in homogenization cycle to increase enzyme yield has proven to be successful. Triple-loop homogenization has shown three times higher proteolytic activity than non-homogenized sample. 2005 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/5270/1/MelissaLohPeiShian2005_PilotScaleExtractionOfProteolyticEnzyme.pdf Loh, Melissa Pei Shian and Syamsul Baharil, Nur Aishah and Ting, Lee Yu and M., Taher and Abdul Majid, Fadzilah Adibah (2005) Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus). In: 2nd International Conference on Chemical and Bioprocess Engineering in conjunction with 19th Symposium of Malaysian Chemical Engineers (SOMChE 2005), 8-10 December 2005, Kota Kinabalu, Sabah. |
| spellingShingle | T Technology (General) Loh, Melissa Pei Shian Syamsul Baharil, Nur Aishah Ting, Lee Yu M., Taher Abdul Majid, Fadzilah Adibah Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus) |
| title | Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus) |
| title_full | Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus) |
| title_fullStr | Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus) |
| title_full_unstemmed | Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus) |
| title_short | Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus) |
| title_sort | pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus) |
| topic | T Technology (General) |
| url | http://eprints.utm.my/5270/ http://eprints.utm.my/5270/1/MelissaLohPeiShian2005_PilotScaleExtractionOfProteolyticEnzyme.pdf |