Phenyl boronate column fractionation of high molecular weight RNA
The interactions of borate ion with 1,2-cis-diol groups are useful in fractionating mixtures of biological molecules. Separation of RNAs, up to the size of tRNAs, from other biomolecules using phenyl boronate agarose (PBA) column has been shown. In this work, we used phenyl-boronate agarose column t...
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| Format: | Article |
| Language: | English |
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Faculty of Chemical & Natural Resources Engineering
1989
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| Online Access: | http://eprints.utm.my/5214/ http://eprints.utm.my/5214/1/FazilahAbdulLatif1989_PhenylBoronateColumnFractionation.pdf |
| _version_ | 1848890995690700800 |
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| author | Abdul Latif, Fazilah |
| author_facet | Abdul Latif, Fazilah |
| author_sort | Abdul Latif, Fazilah |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | The interactions of borate ion with 1,2-cis-diol groups are useful in fractionating mixtures of biological molecules. Separation of RNAs, up to the size of tRNAs, from other biomolecules using phenyl boronate agarose (PBA) column has been shown. In this work, we used phenyl-boronate agarose column to separate normal E. coli 16S ribosomal RNA from 16S ribosomal RNA with a blocked 3'-terminus. The blocking group was a 3',5'-cytidine (5'-32p)-bisphosphate. Using a 30 um boronate/ml of agarose (PBA-30) column we obtained reproducible separations at 22C. The results show that this method is useful in preparing high molecular weight RNA of high specific activity for sequence analysis. It is also effective for separation of nuclease digestion products. |
| first_indexed | 2025-11-15T20:50:56Z |
| format | Article |
| id | utm-5214 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:50:56Z |
| publishDate | 1989 |
| publisher | Faculty of Chemical & Natural Resources Engineering |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-52142010-06-01T15:27:37Z http://eprints.utm.my/5214/ Phenyl boronate column fractionation of high molecular weight RNA Abdul Latif, Fazilah T Technology (General) The interactions of borate ion with 1,2-cis-diol groups are useful in fractionating mixtures of biological molecules. Separation of RNAs, up to the size of tRNAs, from other biomolecules using phenyl boronate agarose (PBA) column has been shown. In this work, we used phenyl-boronate agarose column to separate normal E. coli 16S ribosomal RNA from 16S ribosomal RNA with a blocked 3'-terminus. The blocking group was a 3',5'-cytidine (5'-32p)-bisphosphate. Using a 30 um boronate/ml of agarose (PBA-30) column we obtained reproducible separations at 22C. The results show that this method is useful in preparing high molecular weight RNA of high specific activity for sequence analysis. It is also effective for separation of nuclease digestion products. Faculty of Chemical & Natural Resources Engineering 1989 Article PeerReviewed application/pdf en http://eprints.utm.my/5214/1/FazilahAbdulLatif1989_PhenylBoronateColumnFractionation.pdf Abdul Latif, Fazilah (1989) Phenyl boronate column fractionation of high molecular weight RNA. Buletin FKKKSA, 3 (2). pp. 11-19. ISSN 0128-0740 |
| spellingShingle | T Technology (General) Abdul Latif, Fazilah Phenyl boronate column fractionation of high molecular weight RNA |
| title | Phenyl boronate column fractionation of high molecular weight RNA |
| title_full | Phenyl boronate column fractionation of high molecular weight RNA |
| title_fullStr | Phenyl boronate column fractionation of high molecular weight RNA |
| title_full_unstemmed | Phenyl boronate column fractionation of high molecular weight RNA |
| title_short | Phenyl boronate column fractionation of high molecular weight RNA |
| title_sort | phenyl boronate column fractionation of high molecular weight rna |
| topic | T Technology (General) |
| url | http://eprints.utm.my/5214/ http://eprints.utm.my/5214/1/FazilahAbdulLatif1989_PhenylBoronateColumnFractionation.pdf |