Antithrombin protein from hirudinaria manillensis: Preliminary studies on isolation, sequencing of the antithrombin protein and cloning of the desired gene
Salivary secretions from leeches have been known to possess anticoagulant activity. Studies have been conducted to determine the amino acid sequences and isolation method of this protein. As most studies involved the European leech, Hirudo medicinalis, little studies have been conducted on Hirudinar...
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| Format: | Conference or Workshop Item |
| Language: | English |
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1997
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| Online Access: | http://eprints.utm.my/4704/ http://eprints.utm.my/4704/1/LooiYatSeong1997_AntithrombinProteinFromHirudinariaManillensis.pdf |
| _version_ | 1848890869925543936 |
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| author | Looi, Yat Seong Abd. Latif, Fazilah Norbib, Normah Mohd. Said, Nazlan |
| author_facet | Looi, Yat Seong Abd. Latif, Fazilah Norbib, Normah Mohd. Said, Nazlan |
| author_sort | Looi, Yat Seong |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | Salivary secretions from leeches have been known to possess anticoagulant activity. Studies have been conducted to determine the amino acid sequences and isolation method of this protein. As most studies involved the European leech, Hirudo medicinalis, little studies have been conducted on Hirudinaria manillensis. With the trend of coronary heart disease and other related phatological diseases began to rise, antithrombin protein has potential in the pharmaceutical field. Thus, it is important for mast production of this protein. Molecular biology techniques have made possible cloning and expression of the genes responsible. However, isolation using chromatographic techniques together with the low yield and low activity of the recombinant antithrombin protein have prompt us to look into other alternative of purification and expression system. The N-terminal amino acid sequences of the antithrombin protein from Malaysian local leech were also studied.
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| first_indexed | 2025-11-15T20:48:56Z |
| format | Conference or Workshop Item |
| id | utm-4704 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:48:56Z |
| publishDate | 1997 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-47042017-07-24T06:41:57Z http://eprints.utm.my/4704/ Antithrombin protein from hirudinaria manillensis: Preliminary studies on isolation, sequencing of the antithrombin protein and cloning of the desired gene Looi, Yat Seong Abd. Latif, Fazilah Norbib, Normah Mohd. Said, Nazlan T Technology (General) Salivary secretions from leeches have been known to possess anticoagulant activity. Studies have been conducted to determine the amino acid sequences and isolation method of this protein. As most studies involved the European leech, Hirudo medicinalis, little studies have been conducted on Hirudinaria manillensis. With the trend of coronary heart disease and other related phatological diseases began to rise, antithrombin protein has potential in the pharmaceutical field. Thus, it is important for mast production of this protein. Molecular biology techniques have made possible cloning and expression of the genes responsible. However, isolation using chromatographic techniques together with the low yield and low activity of the recombinant antithrombin protein have prompt us to look into other alternative of purification and expression system. The N-terminal amino acid sequences of the antithrombin protein from Malaysian local leech were also studied. 1997 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/4704/1/LooiYatSeong1997_AntithrombinProteinFromHirudinariaManillensis.pdf Looi, Yat Seong and Abd. Latif, Fazilah and Norbib, Normah and Mohd. Said, Nazlan (1997) Antithrombin protein from hirudinaria manillensis: Preliminary studies on isolation, sequencing of the antithrombin protein and cloning of the desired gene. In: Regional Symposium on Chemical Engineering 1997 in conjunction with 13th Symposium of Malaysian Chemical Engineers, 13-15 October 1997, Hyatt Regency, Johor, Malaysia. |
| spellingShingle | T Technology (General) Looi, Yat Seong Abd. Latif, Fazilah Norbib, Normah Mohd. Said, Nazlan Antithrombin protein from hirudinaria manillensis: Preliminary studies on isolation, sequencing of the antithrombin protein and cloning of the desired gene |
| title | Antithrombin protein from hirudinaria manillensis: Preliminary studies on isolation, sequencing of the antithrombin protein and cloning of the desired gene |
| title_full | Antithrombin protein from hirudinaria manillensis: Preliminary studies on isolation, sequencing of the antithrombin protein and cloning of the desired gene |
| title_fullStr | Antithrombin protein from hirudinaria manillensis: Preliminary studies on isolation, sequencing of the antithrombin protein and cloning of the desired gene |
| title_full_unstemmed | Antithrombin protein from hirudinaria manillensis: Preliminary studies on isolation, sequencing of the antithrombin protein and cloning of the desired gene |
| title_short | Antithrombin protein from hirudinaria manillensis: Preliminary studies on isolation, sequencing of the antithrombin protein and cloning of the desired gene |
| title_sort | antithrombin protein from hirudinaria manillensis: preliminary studies on isolation, sequencing of the antithrombin protein and cloning of the desired gene |
| topic | T Technology (General) |
| url | http://eprints.utm.my/4704/ http://eprints.utm.my/4704/1/LooiYatSeong1997_AntithrombinProteinFromHirudinariaManillensis.pdf |