Review : overexpression of protein under transcriptional regulation of lambda pL promoter system in Escherichia coli: consequences and bioprocess improvement approaches
The attraction of employing thermal induction system in the production of heterologous protein is mainly due to 1) a higher expressed protein titer in comparison to constitutive system, and 2) a cleaner process since no chemical inducing agent is added. However, problems pertaining to high temperatu...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Faculty of Chemical and Natural Resources Engineering, UTM
2007
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| Online Access: | http://eprints.utm.my/4633/ http://eprints.utm.my/4633/1/FirdausiRazali2007_ReviewOverexpressionofProteinUnderTranscriptional.pdf http://eprints.utm.my/4633/2/vol%201%2022-39.pdf |
| Summary: | The attraction of employing thermal induction system in the production of heterologous protein is mainly due to 1) a higher expressed protein titer in comparison to constitutive system, and 2) a cleaner process since no chemical inducing agent is added. However, problems pertaining to high temperature and protein overexpression such as inclusion bodies formations, metabolic overload on the host, stress-related proteolysis on the expressed protein, and enhanced acetic acid secretion are commonly encountered. Several bioprocess approaches that include temperature upshift schemes, feeding strategies, timing of induction, two-stage culture mode, and stabilization of plasmid had been implemented to minimize some of these problems. The approaches are reviewed in this article.
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