Degradation of acid red 27 by the recombinant flavin reductase
This paper focuses on the degradation of azo compound C.I. Acid Red 27 (AR27, amaranth) by the recombinant enzyme flavin reductase (FRE) from Citrobacter freundii strain A1. The enzyme was obtained via re-transformation of recombinant plasmid pET-43.1c(+)freBP containing the flavin reductase gene (f...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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2006
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| Online Access: | http://eprints.utm.my/325/ http://eprints.utm.my/325/1/KUSTEM_2006_Paper.pdf |
| _version_ | 1848889897138520064 |
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| author | Abdul Wahab, Mohd. Firdaus Abdul Rashid, Noor Aini Mohd. Yusoff, Abdull Rahim |
| author_facet | Abdul Wahab, Mohd. Firdaus Abdul Rashid, Noor Aini Mohd. Yusoff, Abdull Rahim |
| author_sort | Abdul Wahab, Mohd. Firdaus |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | This paper focuses on the degradation of azo compound C.I. Acid Red 27 (AR27, amaranth) by the recombinant enzyme flavin reductase (FRE) from Citrobacter freundii strain A1. The enzyme was obtained via re-transformation of recombinant plasmid pET-43.1c(+)freBP containing the flavin reductase gene (fre) into E. coli NovaBlue. The plasmid was subsequently transformed into E. coli BL21(DE3)pLysS for overexpression of FRE fusion protein. Prior to that, the stability of fre gene was first verified using PCR amplification and also sequencing of the nucleotides and consequently compared with the original fre gene sequence from C. freundii A1. The protein was expressed as inclusion bodies and was isolated and refolded in order to obtain a properly-folded and active flavin reductase. The activity and the protein yield were monitored using a modified flavin reductase assay and Bradford assay respectively. The active enzyme was further subjected to the degradation of AR27 and the degradation profile was constructed. |
| first_indexed | 2025-11-15T20:33:28Z |
| format | Conference or Workshop Item |
| id | utm-325 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:33:28Z |
| publishDate | 2006 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-3252017-09-07T03:22:24Z http://eprints.utm.my/325/ Degradation of acid red 27 by the recombinant flavin reductase Abdul Wahab, Mohd. Firdaus Abdul Rashid, Noor Aini Mohd. Yusoff, Abdull Rahim QR Microbiology This paper focuses on the degradation of azo compound C.I. Acid Red 27 (AR27, amaranth) by the recombinant enzyme flavin reductase (FRE) from Citrobacter freundii strain A1. The enzyme was obtained via re-transformation of recombinant plasmid pET-43.1c(+)freBP containing the flavin reductase gene (fre) into E. coli NovaBlue. The plasmid was subsequently transformed into E. coli BL21(DE3)pLysS for overexpression of FRE fusion protein. Prior to that, the stability of fre gene was first verified using PCR amplification and also sequencing of the nucleotides and consequently compared with the original fre gene sequence from C. freundii A1. The protein was expressed as inclusion bodies and was isolated and refolded in order to obtain a properly-folded and active flavin reductase. The activity and the protein yield were monitored using a modified flavin reductase assay and Bradford assay respectively. The active enzyme was further subjected to the degradation of AR27 and the degradation profile was constructed. 2006 Conference or Workshop Item NonPeerReviewed application/pdf en http://eprints.utm.my/325/1/KUSTEM_2006_Paper.pdf Abdul Wahab, Mohd. Firdaus and Abdul Rashid, Noor Aini and Mohd. Yusoff, Abdull Rahim (2006) Degradation of acid red 27 by the recombinant flavin reductase. In: KUSTEM 5th Annual Seminar, 2-3 May 2006., Primula Beach Resort, Kuala Terengganu.. |
| spellingShingle | QR Microbiology Abdul Wahab, Mohd. Firdaus Abdul Rashid, Noor Aini Mohd. Yusoff, Abdull Rahim Degradation of acid red 27 by the recombinant flavin reductase |
| title | Degradation of acid red 27 by the recombinant flavin reductase |
| title_full | Degradation of acid red 27 by the recombinant flavin reductase |
| title_fullStr | Degradation of acid red 27 by the recombinant flavin reductase |
| title_full_unstemmed | Degradation of acid red 27 by the recombinant flavin reductase |
| title_short | Degradation of acid red 27 by the recombinant flavin reductase |
| title_sort | degradation of acid red 27 by the recombinant flavin reductase |
| topic | QR Microbiology |
| url | http://eprints.utm.my/325/ http://eprints.utm.my/325/1/KUSTEM_2006_Paper.pdf |