Combined In-Fermenter Extraction and Cross-Flow Microfiltration for Improved Inclusion Body Processing

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Combined In-Fermenter Extraction and Cross-Flow Microfiltration for Improved Inclusion Body Processing

In this study we demonstrate a new in-fermenter chemical extraction procedure that degrades the cell wall of Escherichia coli and releases inclusion bodies (IBs) into the fermentation medium. We then prove that cross-flow microfiltration can be used to remove 91% of soluble contaminants from the rel...

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Bibliographic Details
Main Authors:	Chew, Tin Lee, Morreale, Giacomo, Middelberg, Anton P. J.
Format:	Article
Language:	English
Published:	Wiley-Blackwell 2004
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/3085/ http://eprints.utm.my/3085/1/chewtinlee2004_combined%20in-fermenterextraction.pdf

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