Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS)
An experiment was carried out to study the fundamental factors that affect the growth of Spodoptera frugiperda Sf-9 insect cells. Initial cell density, spent medium carry over and inoculum phase withdrawal significantly influenced the growth kinetics of Sf-9 cells. The percentage of cells infected w...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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2004
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| Subjects: | |
| Online Access: | http://eprints.utm.my/3063/ http://eprints.utm.my/3063/1/50_%28C5-4%29.pdf |
| _version_ | 1848890498226323456 |
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| author | Yap, Wei Ney Abdul-Rahman, Badarulhisam Abdul-Aziz, Azila |
| author_facet | Yap, Wei Ney Abdul-Rahman, Badarulhisam Abdul-Aziz, Azila |
| author_sort | Yap, Wei Ney |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | An experiment was carried out to study the fundamental factors that affect the growth of Spodoptera frugiperda Sf-9 insect cells. Initial cell density, spent medium carry over and inoculum phase withdrawal significantly influenced the growth kinetics of Sf-9 cells. The percentage of cells infected with Autographa californica multiply-enveloped Nuclear Polyhedrosis Virus (AcMNPV) was obviously affected by spent medium carry over. On the other hand, initial cell density and Multiplicities of Infection (MOI) have minimal influence on infectivity percentage. |
| first_indexed | 2025-11-15T20:43:01Z |
| format | Conference or Workshop Item |
| id | utm-3063 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:43:01Z |
| publishDate | 2004 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-30632017-08-30T07:03:16Z http://eprints.utm.my/3063/ Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS) Yap, Wei Ney Abdul-Rahman, Badarulhisam Abdul-Aziz, Azila QH301 Biology An experiment was carried out to study the fundamental factors that affect the growth of Spodoptera frugiperda Sf-9 insect cells. Initial cell density, spent medium carry over and inoculum phase withdrawal significantly influenced the growth kinetics of Sf-9 cells. The percentage of cells infected with Autographa californica multiply-enveloped Nuclear Polyhedrosis Virus (AcMNPV) was obviously affected by spent medium carry over. On the other hand, initial cell density and Multiplicities of Infection (MOI) have minimal influence on infectivity percentage. 2004-12-08 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/3063/1/50_%28C5-4%29.pdf Yap, Wei Ney and Abdul-Rahman, Badarulhisam and Abdul-Aziz, Azila (2004) Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS). In: National Postgraduate Colloquium, 8-9 December 2004, The Gurnay Resort Hotel & Residence, Penang. |
| spellingShingle | QH301 Biology Yap, Wei Ney Abdul-Rahman, Badarulhisam Abdul-Aziz, Azila Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS) |
| title | Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS) |
| title_full | Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS) |
| title_fullStr | Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS) |
| title_full_unstemmed | Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS) |
| title_short | Cell Culture Optmization for the Baculovirus Expression Vector System (BEVS) |
| title_sort | cell culture optmization for the baculovirus expression vector system (bevs) |
| topic | QH301 Biology |
| url | http://eprints.utm.my/3063/ http://eprints.utm.my/3063/1/50_%28C5-4%29.pdf |