Degradation of thiosulfate by sulfide-oxidizing enzyme produced by bacteria locally isolated from effective microorganism active solution (EMAS)
A potential aerobic sulfur-oxidizing bacterium (SOB) that was believed to be sulfide-oxidizing enzyme producing strain was previously isolated from Effective Microorganism Active Solution (EMAS), currently known as SO2. This gram negative bacterium was capable of growing autotrophically in sulfur-o...
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| Format: | Article |
| Language: | English |
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School of Postgraduate Studies, UTM
2006
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| Online Access: | http://eprints.utm.my/3046/ http://eprints.utm.my/3046/1/RPCES_2006_Madihah2.pdf |
| _version_ | 1848890494183014400 |
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| author | Ashaari, Nur Suhanawati Md. Salleh, Madihah Md. Illias, Rosli |
| author_facet | Ashaari, Nur Suhanawati Md. Salleh, Madihah Md. Illias, Rosli |
| author_sort | Ashaari, Nur Suhanawati |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | A potential aerobic sulfur-oxidizing bacterium (SOB) that was believed to be sulfide-oxidizing enzyme producing strain was previously isolated from Effective Microorganism Active Solution (EMAS), currently known as SO2. This gram negative bacterium was capable of growing autotrophically in sulfur-oxidizer medium, containing 4mM sodium thiosulfate that serves as an energy sources and electron donor. Sulfate ions were the expected end product of sulfide oxidation catalyzed by extracellular sulfide-oxidizing enzyme. The aims of this study are to identify and characterize the SO2 and to induce the activity of sulfide-oxidizing enzyme in this strain. The activity of sulfide-oxidizing enzyme was determined spectrophotometrically by measuring the increase of sulfate production using BaCl2 solution, while the oxidation of thiosulfate was colorimetrically determined at 460 nm. One unit of sulfide-oxidizing activity was defined as amount of enzyme required to produce 1 µmol sulfate per min per mL (U). The maximum sulfide-oxidizing activity (0.064 U) was achieved when the strain was grown at pH 5.0, 30˚C in medium containing 1% (w/v) peptone as nitrogen sources after 15 hours incubation. The specific growth rate of this strain at this condition was 0.1552 h-1, with doubling time value of 4.47 h. |
| first_indexed | 2025-11-15T20:42:58Z |
| format | Article |
| id | utm-3046 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:42:58Z |
| publishDate | 2006 |
| publisher | School of Postgraduate Studies, UTM |
| recordtype | eprints |
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| spelling | utm-30462017-10-24T01:05:17Z http://eprints.utm.my/3046/ Degradation of thiosulfate by sulfide-oxidizing enzyme produced by bacteria locally isolated from effective microorganism active solution (EMAS) Ashaari, Nur Suhanawati Md. Salleh, Madihah Md. Illias, Rosli Q Science (General) A potential aerobic sulfur-oxidizing bacterium (SOB) that was believed to be sulfide-oxidizing enzyme producing strain was previously isolated from Effective Microorganism Active Solution (EMAS), currently known as SO2. This gram negative bacterium was capable of growing autotrophically in sulfur-oxidizer medium, containing 4mM sodium thiosulfate that serves as an energy sources and electron donor. Sulfate ions were the expected end product of sulfide oxidation catalyzed by extracellular sulfide-oxidizing enzyme. The aims of this study are to identify and characterize the SO2 and to induce the activity of sulfide-oxidizing enzyme in this strain. The activity of sulfide-oxidizing enzyme was determined spectrophotometrically by measuring the increase of sulfate production using BaCl2 solution, while the oxidation of thiosulfate was colorimetrically determined at 460 nm. One unit of sulfide-oxidizing activity was defined as amount of enzyme required to produce 1 µmol sulfate per min per mL (U). The maximum sulfide-oxidizing activity (0.064 U) was achieved when the strain was grown at pH 5.0, 30˚C in medium containing 1% (w/v) peptone as nitrogen sources after 15 hours incubation. The specific growth rate of this strain at this condition was 0.1552 h-1, with doubling time value of 4.47 h. School of Postgraduate Studies, UTM 2006-07-25 Article NonPeerReviewed application/pdf en http://eprints.utm.my/3046/1/RPCES_2006_Madihah2.pdf Ashaari, Nur Suhanawati and Md. Salleh, Madihah and Md. Illias, Rosli (2006) Degradation of thiosulfate by sulfide-oxidizing enzyme produced by bacteria locally isolated from effective microorganism active solution (EMAS). Regional Postgraduate Conference on Engineering and Science . pp. 631-636. |
| spellingShingle | Q Science (General) Ashaari, Nur Suhanawati Md. Salleh, Madihah Md. Illias, Rosli Degradation of thiosulfate by sulfide-oxidizing enzyme produced by bacteria locally isolated from effective microorganism active solution (EMAS) |
| title | Degradation of thiosulfate by sulfide-oxidizing enzyme produced by bacteria locally isolated from effective microorganism active solution (EMAS) |
| title_full | Degradation of thiosulfate by sulfide-oxidizing enzyme produced by bacteria locally isolated from effective microorganism active solution (EMAS) |
| title_fullStr | Degradation of thiosulfate by sulfide-oxidizing enzyme produced by bacteria locally isolated from effective microorganism active solution (EMAS) |
| title_full_unstemmed | Degradation of thiosulfate by sulfide-oxidizing enzyme produced by bacteria locally isolated from effective microorganism active solution (EMAS) |
| title_short | Degradation of thiosulfate by sulfide-oxidizing enzyme produced by bacteria locally isolated from effective microorganism active solution (EMAS) |
| title_sort | degradation of thiosulfate by sulfide-oxidizing enzyme produced by bacteria locally isolated from effective microorganism active solution (emas) |
| topic | Q Science (General) |
| url | http://eprints.utm.my/3046/ http://eprints.utm.my/3046/1/RPCES_2006_Madihah2.pdf |