Decolourization of Azo Dye Direct Blue 15 Using Batch Culture of Klebsiella sp

Nowadays, the development of textile industries are mushrooming and the pollution resulting from the dye-wastewater is alarming. Colour is recognized as the first contaminant from the dye containing wastewater. The present study reports the decolourization of Direct Blue 15 by Klebsiella sp., origi...

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Main Authors: Chong, Chun Siong, Ibrahim, Zaharah, Md Salleh, Madihah, Abdulrashid, Noor Aini, Yahya, Adibah, Wui, Jin Wong
Format: Article
Language:English
Published: School of Postgraduate Studies, UTM 2006
Subjects:
Online Access:http://eprints.utm.my/3043/
http://eprints.utm.my/3043/1/RPCES_2006_Dr_Adibah.pdf
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author Chong, Chun Siong
Ibrahim, Zaharah
Md Salleh, Madihah
Abdulrashid, Noor Aini
Yahya, Adibah
Wui, Jin Wong
author_facet Chong, Chun Siong
Ibrahim, Zaharah
Md Salleh, Madihah
Abdulrashid, Noor Aini
Yahya, Adibah
Wui, Jin Wong
author_sort Chong, Chun Siong
building UTeM Institutional Repository
collection Online Access
description Nowadays, the development of textile industries are mushrooming and the pollution resulting from the dye-wastewater is alarming. Colour is recognized as the first contaminant from the dye containing wastewater. The present study reports the decolourization of Direct Blue 15 by Klebsiella sp., originated from textile wastewater plant. The important parameters, including co-substrates, aeration, starting pH of culture and temperature for decolourization, were investigated. Mechanism of colour removal either by bioadsorption or biodegradation was determined. Amongst the co-substrates, glucose (0.2% w/v) was the preferred co-substrate for decolourization of Direct Blue 15. The optimal decolourization occurred under partial anaerobic condition at starting pH of about 6.0. Decolourization of Direct Blue 15 was most efficient at 45oC. Under the optimum conditions, it was found that the COD removal was 53.6%. Dye decolourization (81.9%) was successfully achieved within 24 hours; 0.03% was due to the bioadsorption and 81.87% was due to the biodegradation by Klebsiella sp., while the product of degradation of azo dye, sulfanilic acid, was detected using HPLC.
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spelling utm-30432017-10-19T05:32:15Z http://eprints.utm.my/3043/ Decolourization of Azo Dye Direct Blue 15 Using Batch Culture of Klebsiella sp Chong, Chun Siong Ibrahim, Zaharah Md Salleh, Madihah Abdulrashid, Noor Aini Yahya, Adibah Wui, Jin Wong Q Science (General) Nowadays, the development of textile industries are mushrooming and the pollution resulting from the dye-wastewater is alarming. Colour is recognized as the first contaminant from the dye containing wastewater. The present study reports the decolourization of Direct Blue 15 by Klebsiella sp., originated from textile wastewater plant. The important parameters, including co-substrates, aeration, starting pH of culture and temperature for decolourization, were investigated. Mechanism of colour removal either by bioadsorption or biodegradation was determined. Amongst the co-substrates, glucose (0.2% w/v) was the preferred co-substrate for decolourization of Direct Blue 15. The optimal decolourization occurred under partial anaerobic condition at starting pH of about 6.0. Decolourization of Direct Blue 15 was most efficient at 45oC. Under the optimum conditions, it was found that the COD removal was 53.6%. Dye decolourization (81.9%) was successfully achieved within 24 hours; 0.03% was due to the bioadsorption and 81.87% was due to the biodegradation by Klebsiella sp., while the product of degradation of azo dye, sulfanilic acid, was detected using HPLC. School of Postgraduate Studies, UTM 2006-07-25 Article NonPeerReviewed application/pdf en http://eprints.utm.my/3043/1/RPCES_2006_Dr_Adibah.pdf Chong, Chun Siong and Ibrahim, Zaharah and Md Salleh, Madihah and Abdulrashid, Noor Aini and Yahya, Adibah and Wui, Jin Wong (2006) Decolourization of Azo Dye Direct Blue 15 Using Batch Culture of Klebsiella sp. Regional Postgraduate Conference on Engineering and Science .
spellingShingle Q Science (General)
Chong, Chun Siong
Ibrahim, Zaharah
Md Salleh, Madihah
Abdulrashid, Noor Aini
Yahya, Adibah
Wui, Jin Wong
Decolourization of Azo Dye Direct Blue 15 Using Batch Culture of Klebsiella sp
title Decolourization of Azo Dye Direct Blue 15 Using Batch Culture of Klebsiella sp
title_full Decolourization of Azo Dye Direct Blue 15 Using Batch Culture of Klebsiella sp
title_fullStr Decolourization of Azo Dye Direct Blue 15 Using Batch Culture of Klebsiella sp
title_full_unstemmed Decolourization of Azo Dye Direct Blue 15 Using Batch Culture of Klebsiella sp
title_short Decolourization of Azo Dye Direct Blue 15 Using Batch Culture of Klebsiella sp
title_sort decolourization of azo dye direct blue 15 using batch culture of klebsiella sp
topic Q Science (General)
url http://eprints.utm.my/3043/
http://eprints.utm.my/3043/1/RPCES_2006_Dr_Adibah.pdf