Alkaline Phosphatase Purification from Bacillus megaterium
The aim of this study was to isolate and purify an alkaline phosphatase (ALPs) from Bacillus megaterium. The purified enzyme was established by gel filtration chromatography and high performance liquid chromatography (HPLC). Then, followed by non-denaturing gel electrophoresis to separate the ALPs f...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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2012
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| Online Access: | http://eprints.utm.my/3041/ http://eprints.utm.my/3041/1/final_prosiding_husza.pdf |
| _version_ | 1848890493391339520 |
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| author | Hussin, Huszalina Hsu, W .C. Nebenfuhr, M. Papantoniou, C. |
| author_facet | Hussin, Huszalina Hsu, W .C. Nebenfuhr, M. Papantoniou, C. |
| author_sort | Hussin, Huszalina |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | The aim of this study was to isolate and purify an alkaline phosphatase (ALPs) from Bacillus megaterium. The purified enzyme was established by gel filtration chromatography and high performance liquid chromatography (HPLC). Then, followed by non-denaturing gel electrophoresis to separate the ALPs from described purification technique. The purified enzyme had the following properties: ALPs concentration obtained from enzyme assay culture supernatant was 13.453ïg and selected purification techniques of gel filtration and HPLC were both 19.74ïg and 23.52ïg respectively. The enzyme’s activity and specific activity from culture supernatant were both 0.493 U/ml and 36.3 U/mg respectively. |
| first_indexed | 2025-11-15T20:42:57Z |
| format | Article |
| id | utm-3041 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:42:57Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-30412017-10-19T05:29:35Z http://eprints.utm.my/3041/ Alkaline Phosphatase Purification from Bacillus megaterium Hussin, Huszalina Hsu, W .C. Nebenfuhr, M. Papantoniou, C. QR355 Virology The aim of this study was to isolate and purify an alkaline phosphatase (ALPs) from Bacillus megaterium. The purified enzyme was established by gel filtration chromatography and high performance liquid chromatography (HPLC). Then, followed by non-denaturing gel electrophoresis to separate the ALPs from described purification technique. The purified enzyme had the following properties: ALPs concentration obtained from enzyme assay culture supernatant was 13.453ïg and selected purification techniques of gel filtration and HPLC were both 19.74ïg and 23.52ïg respectively. The enzyme’s activity and specific activity from culture supernatant were both 0.493 U/ml and 36.3 U/mg respectively. 2012 Article NonPeerReviewed application/pdf en http://eprints.utm.my/3041/1/final_prosiding_husza.pdf Hussin, Huszalina and Hsu, W .C. and Nebenfuhr, M. and Papantoniou, C. (2012) Alkaline Phosphatase Purification from Bacillus megaterium. Kustem Journal . |
| spellingShingle | QR355 Virology Hussin, Huszalina Hsu, W .C. Nebenfuhr, M. Papantoniou, C. Alkaline Phosphatase Purification from Bacillus megaterium |
| title | Alkaline Phosphatase Purification from Bacillus megaterium |
| title_full | Alkaline Phosphatase Purification from Bacillus megaterium |
| title_fullStr | Alkaline Phosphatase Purification from Bacillus megaterium |
| title_full_unstemmed | Alkaline Phosphatase Purification from Bacillus megaterium |
| title_short | Alkaline Phosphatase Purification from Bacillus megaterium |
| title_sort | alkaline phosphatase purification from bacillus megaterium |
| topic | QR355 Virology |
| url | http://eprints.utm.my/3041/ http://eprints.utm.my/3041/1/final_prosiding_husza.pdf |