Synthesis of 2-O-a-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from bacillus sp.
Cyclomaltodextrin glucanotransferase (CGTase) [EC 2.4.1.19] was found to catalyze the transglycosylation from α-cyclodextrin (α-CD) to l-ascorbic acid (AA). A main product formed by this reaction was identified as 2-O-α-d-glucopyranosyl l-ascorbic acid (AA-2G) with the enzymatic hydrolysis and ultra...
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| Format: | Monograph |
| Language: | English |
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Universiti Teknologi Malaysia
2006
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| Online Access: | http://eprints.utm.my/2794/ http://eprints.utm.my/2794/1/74184.pdf |
| _version_ | 1848890433808105472 |
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| author | Abd. Rahman, Roshanida |
| author_facet | Abd. Rahman, Roshanida |
| author_sort | Abd. Rahman, Roshanida |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | Cyclomaltodextrin glucanotransferase (CGTase) [EC 2.4.1.19] was found to catalyze the transglycosylation from α-cyclodextrin (α-CD) to l-ascorbic acid (AA). A main product formed by this reaction was identified as 2-O-α-d-glucopyranosyl l-ascorbic acid (AA-2G) with the enzymatic hydrolysis and ultraviolet absorption spectra using standard AA-2G. A series of maltooligosaccharide substituted 2-O-derivatives of AA were also detected by HPLC. These were thoroughly hydrolized to AA-2G and glucose by treatment with glucoamylase [EC 3.2.1.3]. A large amount of AA-2G was prepared with 500 g of AA and 1000 g of α-CD. Two hundred and fifty grams of purified AA-2G, the content of which was 97%, was obtained through enzyme reactions and purification with HPLC using an ion-exchange resin. |
| first_indexed | 2025-11-15T20:42:00Z |
| format | Monograph |
| id | utm-2794 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:42:00Z |
| publishDate | 2006 |
| publisher | Universiti Teknologi Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-27942017-06-15T03:06:00Z http://eprints.utm.my/2794/ Synthesis of 2-O-a-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from bacillus sp. Abd. Rahman, Roshanida TP Chemical technology Cyclomaltodextrin glucanotransferase (CGTase) [EC 2.4.1.19] was found to catalyze the transglycosylation from α-cyclodextrin (α-CD) to l-ascorbic acid (AA). A main product formed by this reaction was identified as 2-O-α-d-glucopyranosyl l-ascorbic acid (AA-2G) with the enzymatic hydrolysis and ultraviolet absorption spectra using standard AA-2G. A series of maltooligosaccharide substituted 2-O-derivatives of AA were also detected by HPLC. These were thoroughly hydrolized to AA-2G and glucose by treatment with glucoamylase [EC 3.2.1.3]. A large amount of AA-2G was prepared with 500 g of AA and 1000 g of α-CD. Two hundred and fifty grams of purified AA-2G, the content of which was 97%, was obtained through enzyme reactions and purification with HPLC using an ion-exchange resin. Universiti Teknologi Malaysia 2006-03-31 Monograph NonPeerReviewed application/pdf en http://eprints.utm.my/2794/1/74184.pdf Abd. Rahman, Roshanida (2006) Synthesis of 2-O-a-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from bacillus sp. Project Report. Universiti Teknologi Malaysia, Skudai, Johor. (Unpublished) http://www.tandfonline.com/doi/abs/10.1080/00021369.1991.10870856 |
| spellingShingle | TP Chemical technology Abd. Rahman, Roshanida Synthesis of 2-O-a-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from bacillus sp. |
| title | Synthesis of 2-O-a-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from bacillus sp. |
| title_full | Synthesis of 2-O-a-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from bacillus sp. |
| title_fullStr | Synthesis of 2-O-a-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from bacillus sp. |
| title_full_unstemmed | Synthesis of 2-O-a-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from bacillus sp. |
| title_short | Synthesis of 2-O-a-D-glucopyranosyl L-ascorbic acid by cyclomaltodextrin glucanotransferase from bacillus sp. |
| title_sort | synthesis of 2-o-a-d-glucopyranosyl l-ascorbic acid by cyclomaltodextrin glucanotransferase from bacillus sp. |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/2794/ http://eprints.utm.my/2794/ http://eprints.utm.my/2794/1/74184.pdf |