Cloning and expression of pullulanase gene from locally isolated bacillus SP
Bacterial pullulanase represents one of th e starch-degrading enzymes that are widely used in the starch processing indu stry along with amylases. Amylases hydrolyze a -(1,4 )-glycosidic linkage in starch to produce a mixture of glucose , maltooligo sacchari de and limited a-dextrin. All the r...
| Main Authors: | , , , , , |
|---|---|
| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Teknologi Malaysia
2006
|
| Subjects: | |
| Online Access: | http://eprints.utm.my/2754/ http://eprints.utm.my/2754/1/74189.pdf |
| _version_ | 1848890424426496000 |
|---|---|
| author | Nik Mahmood, Nik Azmi Md. Illias, Rosli Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Shahab, Neelam |
| author_facet | Nik Mahmood, Nik Azmi Md. Illias, Rosli Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Shahab, Neelam |
| author_sort | Nik Mahmood, Nik Azmi |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | Bacterial pullulanase represents one of th e starch-degrading enzymes that are widely used in the starch processing indu stry along with amylases. Amylases hydrolyze a -(1,4 )-glycosidic linkage in starch to produce a mixture of glucose , maltooligo sacchari de and limited a-dextrin. All the remaining a -(1,6)-glycosid ic branches in the products are hydrolyzed by p ullulanase. This is an advantage t o improve glucose production by coupling pullulanase and amylase in the p rocess. As such, many pullulana e enzyme has been isolated and one has been showing optimum pH of 10-10.5 which is suitable for use in dishwasher detergent additive in removal of star ch stain. We have recently iso lated a few bacterias that have shown potentially pullulanase producers by the holo-zone in pullulan-plate assay. One of them, we named Bacillus –1 sho ws a bigger holo-zone among others, Bacillus- 1 is highly active in pH more than 7. The enzyme also shows a mo derate activity to wards starch that may be indicates be side hydrolyzes a -(1,6)-glycosidic linkage in starch, it also hydrolyzes a -(1,4)- glycosidi c simi lar to a -amylase. Unfortunately the enzyme from wild-type bacteria is in lower yield an d in this studies, we intend to clone and sequence the pullulanase gene and also expressed the gene in a high expression system to be able to produce in a high yield before characterizing expressed protein. |
| first_indexed | 2025-11-15T20:41:51Z |
| format | Monograph |
| id | utm-2754 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:41:51Z |
| publishDate | 2006 |
| publisher | Universiti Teknologi Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-27542017-10-11T04:29:44Z http://eprints.utm.my/2754/ Cloning and expression of pullulanase gene from locally isolated bacillus SP Nik Mahmood, Nik Azmi Md. Illias, Rosli Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Shahab, Neelam Q Science (General) Bacterial pullulanase represents one of th e starch-degrading enzymes that are widely used in the starch processing indu stry along with amylases. Amylases hydrolyze a -(1,4 )-glycosidic linkage in starch to produce a mixture of glucose , maltooligo sacchari de and limited a-dextrin. All the remaining a -(1,6)-glycosid ic branches in the products are hydrolyzed by p ullulanase. This is an advantage t o improve glucose production by coupling pullulanase and amylase in the p rocess. As such, many pullulana e enzyme has been isolated and one has been showing optimum pH of 10-10.5 which is suitable for use in dishwasher detergent additive in removal of star ch stain. We have recently iso lated a few bacterias that have shown potentially pullulanase producers by the holo-zone in pullulan-plate assay. One of them, we named Bacillus –1 sho ws a bigger holo-zone among others, Bacillus- 1 is highly active in pH more than 7. The enzyme also shows a mo derate activity to wards starch that may be indicates be side hydrolyzes a -(1,6)-glycosidic linkage in starch, it also hydrolyzes a -(1,4)- glycosidi c simi lar to a -amylase. Unfortunately the enzyme from wild-type bacteria is in lower yield an d in this studies, we intend to clone and sequence the pullulanase gene and also expressed the gene in a high expression system to be able to produce in a high yield before characterizing expressed protein. Universiti Teknologi Malaysia 2006-03-31 Monograph NonPeerReviewed application/pdf en http://eprints.utm.my/2754/1/74189.pdf Nik Mahmood, Nik Azmi and Md. Illias, Rosli and Md. Salleh, Madihah and Hassan, Osman and Kamaruddin, Kamarulzaman and Shahab, Neelam (2006) Cloning and expression of pullulanase gene from locally isolated bacillus SP. Project Report. Universiti Teknologi Malaysia. (Unpublished) https://www.researchgate.net/publication/254655826_Cloning_and_Expression_of_Pullulanase_a_Gene_from_Locally_Isolated_Bacillus |
| spellingShingle | Q Science (General) Nik Mahmood, Nik Azmi Md. Illias, Rosli Md. Salleh, Madihah Hassan, Osman Kamaruddin, Kamarulzaman Shahab, Neelam Cloning and expression of pullulanase gene from locally isolated bacillus SP |
| title | Cloning and expression of pullulanase gene from locally isolated bacillus SP |
| title_full | Cloning and expression of pullulanase gene from locally isolated bacillus SP |
| title_fullStr | Cloning and expression of pullulanase gene from locally isolated bacillus SP |
| title_full_unstemmed | Cloning and expression of pullulanase gene from locally isolated bacillus SP |
| title_short | Cloning and expression of pullulanase gene from locally isolated bacillus SP |
| title_sort | cloning and expression of pullulanase gene from locally isolated bacillus sp |
| topic | Q Science (General) |
| url | http://eprints.utm.my/2754/ http://eprints.utm.my/2754/ http://eprints.utm.my/2754/1/74189.pdf |