Liquid state bioconversion of palm oil mill effluent for cellulase production: statistical optimization of process conditions
The filamentous fungus Trichoderma harzianum was used for liquid state bioconversion of POME for cellulase production. Statistical optimization was carried out to evaluate the physico-chemical parameters (factors) for maximum cellulase production by 2-level fractional factorial design with six centr...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2006
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| Subjects: | |
| Online Access: | http://eprints.utm.my/234/ http://eprints.utm.my/234/1/MdZahangirAlam2006_Liquidstatebioconversionofpalm.pdf |
| _version_ | 1848889874432655360 |
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| author | Alam, Md. Zahangir Abd. Karim, M. Ismail Hamisan, Ahmad Fadhlan Jamal, Parveen Jalal, K.C.A. |
| author_facet | Alam, Md. Zahangir Abd. Karim, M. Ismail Hamisan, Ahmad Fadhlan Jamal, Parveen Jalal, K.C.A. |
| author_sort | Alam, Md. Zahangir |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | The filamentous fungus Trichoderma harzianum was used for liquid state bioconversion of POME for cellulase production. Statistical optimization was carried out to evaluate the physico-chemical parameters (factors) for maximum cellulase production by 2-level fractional factorial design with six central points. The polynomial regression model was developed using the experimental data including the effects of linear, quadratic and interaction of the factors. The factors involved were substrate (POME) and co-substrate (wheat flour) concentrations, temperature, pH, inoculum and agitation. Statistical analysis showed that the optimum conditions were: temperature of 300C, substrate concentration of 2%, wheat flour concentration of 3%, pH of 4, inoculum of 3% and agitation of 200 rpm. Under these conditions, the model predicted the enzyme production to be about 14 FPU/ml. Analysis of variance (ANOVA) of the design showed a high coefficient of determination (R2) value of 0.99, thus ensuring a high satisfactory adjustment of the quadratic model with the experimental data. |
| first_indexed | 2025-11-15T20:33:07Z |
| format | Conference or Workshop Item |
| id | utm-234 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:33:07Z |
| publishDate | 2006 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-2342017-08-23T07:08:48Z http://eprints.utm.my/234/ Liquid state bioconversion of palm oil mill effluent for cellulase production: statistical optimization of process conditions Alam, Md. Zahangir Abd. Karim, M. Ismail Hamisan, Ahmad Fadhlan Jamal, Parveen Jalal, K.C.A. TP Chemical technology The filamentous fungus Trichoderma harzianum was used for liquid state bioconversion of POME for cellulase production. Statistical optimization was carried out to evaluate the physico-chemical parameters (factors) for maximum cellulase production by 2-level fractional factorial design with six central points. The polynomial regression model was developed using the experimental data including the effects of linear, quadratic and interaction of the factors. The factors involved were substrate (POME) and co-substrate (wheat flour) concentrations, temperature, pH, inoculum and agitation. Statistical analysis showed that the optimum conditions were: temperature of 300C, substrate concentration of 2%, wheat flour concentration of 3%, pH of 4, inoculum of 3% and agitation of 200 rpm. Under these conditions, the model predicted the enzyme production to be about 14 FPU/ml. Analysis of variance (ANOVA) of the design showed a high coefficient of determination (R2) value of 0.99, thus ensuring a high satisfactory adjustment of the quadratic model with the experimental data. 2006-07 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/234/1/MdZahangirAlam2006_Liquidstatebioconversionofpalm.pdf Alam, Md. Zahangir and Abd. Karim, M. Ismail and Hamisan, Ahmad Fadhlan and Jamal, Parveen and Jalal, K.C.A. (2006) Liquid state bioconversion of palm oil mill effluent for cellulase production: statistical optimization of process conditions. In: 1st International Conference on Natural Resources Engineering & Technology 2006, 24-25th July 2006, Putrajaya, Malaysia. |
| spellingShingle | TP Chemical technology Alam, Md. Zahangir Abd. Karim, M. Ismail Hamisan, Ahmad Fadhlan Jamal, Parveen Jalal, K.C.A. Liquid state bioconversion of palm oil mill effluent for cellulase production: statistical optimization of process conditions |
| title | Liquid state bioconversion of palm oil mill effluent for cellulase production: statistical optimization of process conditions |
| title_full | Liquid state bioconversion of palm oil mill effluent for cellulase production: statistical optimization of process conditions |
| title_fullStr | Liquid state bioconversion of palm oil mill effluent for cellulase production: statistical optimization of process conditions |
| title_full_unstemmed | Liquid state bioconversion of palm oil mill effluent for cellulase production: statistical optimization of process conditions |
| title_short | Liquid state bioconversion of palm oil mill effluent for cellulase production: statistical optimization of process conditions |
| title_sort | liquid state bioconversion of palm oil mill effluent for cellulase production: statistical optimization of process conditions |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/234/ http://eprints.utm.my/234/1/MdZahangirAlam2006_Liquidstatebioconversionofpalm.pdf |