Effects of filtration volume using powdered activated charcoal on the recovery of L (+)-lactic acid from sago starch

In this preliminary study, fine powdered activated charcoal (PAC) was used to recover lactic acid from the impurities existed in fermentation broth with different volume size of crude lactic acid. 200 g of activated charcoal in 1N hydrochloric acid (HCl) was packed into glass columns (49mm in diamet...

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Main Authors: Bujang, Kopli, Ang, Shin Ying, Awang Adeni, Dayang Salwani
Format: Conference or Workshop Item
Language:English
Published: 2006
Subjects:
Online Access:http://eprints.utm.my/215/
http://eprints.utm.my/215/1/KopliBBujang2006_Effectsoffiltrationvolumeusing.pdf
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author Bujang, Kopli
Ang, Shin Ying
Awang Adeni, Dayang Salwani
author_facet Bujang, Kopli
Ang, Shin Ying
Awang Adeni, Dayang Salwani
author_sort Bujang, Kopli
building UTeM Institutional Repository
collection Online Access
description In this preliminary study, fine powdered activated charcoal (PAC) was used to recover lactic acid from the impurities existed in fermentation broth with different volume size of crude lactic acid. 200 g of activated charcoal in 1N hydrochloric acid (HCl) was packed into glass columns (49mm in diameter), followed by deionized water until pH around 6. Next, 0.5 L (or 2.5 mL/g PAC) and 1.0 L (or 5.0 mL/g PAC) of autoclaved cell-free fermentation broth was added to the glass columns, followed by 0.5 L and 1.0 L of deionized water respectively. It is observed that the efficiency of UV light absorbing compounds removal at ?200 and ?260 (%) is 94.42, 97.61 and 92.55, 97.56 respectively for 0.5L and 1.0L of fermentation broth. Color removal efficiency is observed to be the same (more than 99%) whereas lactic acid recovery increases from 13.56% to 46.43% with the ascend of sample (fermentation broth) volume loaded. On the other hand, the recovery of lactic acid by deionized water (0.5 and 1.0L) is 84.04% and 51.86% respectively. It gives a total recovery of 97.60 and 98.29% respectively for both parameters. Protein and glucose recove
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spelling utm-2152017-08-23T07:17:36Z http://eprints.utm.my/215/ Effects of filtration volume using powdered activated charcoal on the recovery of L (+)-lactic acid from sago starch Bujang, Kopli Ang, Shin Ying Awang Adeni, Dayang Salwani TP Chemical technology In this preliminary study, fine powdered activated charcoal (PAC) was used to recover lactic acid from the impurities existed in fermentation broth with different volume size of crude lactic acid. 200 g of activated charcoal in 1N hydrochloric acid (HCl) was packed into glass columns (49mm in diameter), followed by deionized water until pH around 6. Next, 0.5 L (or 2.5 mL/g PAC) and 1.0 L (or 5.0 mL/g PAC) of autoclaved cell-free fermentation broth was added to the glass columns, followed by 0.5 L and 1.0 L of deionized water respectively. It is observed that the efficiency of UV light absorbing compounds removal at ?200 and ?260 (%) is 94.42, 97.61 and 92.55, 97.56 respectively for 0.5L and 1.0L of fermentation broth. Color removal efficiency is observed to be the same (more than 99%) whereas lactic acid recovery increases from 13.56% to 46.43% with the ascend of sample (fermentation broth) volume loaded. On the other hand, the recovery of lactic acid by deionized water (0.5 and 1.0L) is 84.04% and 51.86% respectively. It gives a total recovery of 97.60 and 98.29% respectively for both parameters. Protein and glucose recove 2006-07 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/215/1/KopliBBujang2006_Effectsoffiltrationvolumeusing.pdf Bujang, Kopli and Ang, Shin Ying and Awang Adeni, Dayang Salwani (2006) Effects of filtration volume using powdered activated charcoal on the recovery of L (+)-lactic acid from sago starch. In: 1st International Conference on Natural Resources Engineering & Technology 2006, 24-25th July 2006, Putrajaya, Malaysia.
spellingShingle TP Chemical technology
Bujang, Kopli
Ang, Shin Ying
Awang Adeni, Dayang Salwani
Effects of filtration volume using powdered activated charcoal on the recovery of L (+)-lactic acid from sago starch
title Effects of filtration volume using powdered activated charcoal on the recovery of L (+)-lactic acid from sago starch
title_full Effects of filtration volume using powdered activated charcoal on the recovery of L (+)-lactic acid from sago starch
title_fullStr Effects of filtration volume using powdered activated charcoal on the recovery of L (+)-lactic acid from sago starch
title_full_unstemmed Effects of filtration volume using powdered activated charcoal on the recovery of L (+)-lactic acid from sago starch
title_short Effects of filtration volume using powdered activated charcoal on the recovery of L (+)-lactic acid from sago starch
title_sort effects of filtration volume using powdered activated charcoal on the recovery of l (+)-lactic acid from sago starch
topic TP Chemical technology
url http://eprints.utm.my/215/
http://eprints.utm.my/215/1/KopliBBujang2006_Effectsoffiltrationvolumeusing.pdf