The performance of different pre-treatment techniques in the isolation and purification of monoclonal IgG antibody
In this study, the effects of different sample preparation techniques on the separation of monoclonal antibody IgG1 were investigated experimentally. Monoclonal IgG1 was obtained from hybridoma cell line TB/C3 transfected with bcl-2 carrier plasmid, which was grown in serum-free medium. Three differ...
| Main Authors: | , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2006
|
| Subjects: | |
| Online Access: | http://eprints.utm.my/178/ http://eprints.utm.my/178/1/MelissaLohPeiShiana2006_Theperformanceofdifferentpre-treatment.pdf |
| _version_ | 1848889862840647680 |
|---|---|
| author | Loh, Melissa Pei Shian Abdul Majid, Fadzilah Adibah |
| author_facet | Loh, Melissa Pei Shian Abdul Majid, Fadzilah Adibah |
| author_sort | Loh, Melissa Pei Shian |
| building | UTeM Institutional Repository |
| collection | Online Access |
| description | In this study, the effects of different sample preparation techniques on the separation of monoclonal antibody IgG1 were investigated experimentally. Monoclonal IgG1 was obtained from hybridoma cell line TB/C3 transfected with bcl-2 carrier plasmid, which was grown in serum-free medium. Three different pre-treatment techniques prior to Protein G affinity chromatography have been used in order to concentrate and partial purify the monoclonal antibody. The pre-treatments researched in this paper are precipitation of the antibody by ammonium sulfate, dilution of the antibody in the binding buffer of affinity chromatography and ultrafiltration through an Amicon Ultra-15 filter with molecular weight cut-off at 100 kDa. Purification through direct application of the antibody onto the Protein G affinity column without pre-treatments was used as a control method. The results indicate that the ultrafiltration through an Amicon filter was an effective method for both concentration and partial purification of the antibodies in laboratory scale. |
| first_indexed | 2025-11-15T20:32:56Z |
| format | Conference or Workshop Item |
| id | utm-178 |
| institution | Universiti Teknologi Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:32:56Z |
| publishDate | 2006 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | utm-1782017-08-23T08:37:08Z http://eprints.utm.my/178/ The performance of different pre-treatment techniques in the isolation and purification of monoclonal IgG antibody Loh, Melissa Pei Shian Abdul Majid, Fadzilah Adibah QR Microbiology In this study, the effects of different sample preparation techniques on the separation of monoclonal antibody IgG1 were investigated experimentally. Monoclonal IgG1 was obtained from hybridoma cell line TB/C3 transfected with bcl-2 carrier plasmid, which was grown in serum-free medium. Three different pre-treatment techniques prior to Protein G affinity chromatography have been used in order to concentrate and partial purify the monoclonal antibody. The pre-treatments researched in this paper are precipitation of the antibody by ammonium sulfate, dilution of the antibody in the binding buffer of affinity chromatography and ultrafiltration through an Amicon Ultra-15 filter with molecular weight cut-off at 100 kDa. Purification through direct application of the antibody onto the Protein G affinity column without pre-treatments was used as a control method. The results indicate that the ultrafiltration through an Amicon filter was an effective method for both concentration and partial purification of the antibodies in laboratory scale. 2006-07 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/178/1/MelissaLohPeiShiana2006_Theperformanceofdifferentpre-treatment.pdf Loh, Melissa Pei Shian and Abdul Majid, Fadzilah Adibah (2006) The performance of different pre-treatment techniques in the isolation and purification of monoclonal IgG antibody. In: 1st International Conference on Natural Resources Engineering & Technology 2006, 24-25th July 2006, Putrajaya, Malaysia. |
| spellingShingle | QR Microbiology Loh, Melissa Pei Shian Abdul Majid, Fadzilah Adibah The performance of different pre-treatment techniques in the isolation and purification of monoclonal IgG antibody |
| title | The performance of different pre-treatment techniques in the isolation and purification of monoclonal IgG antibody |
| title_full | The performance of different pre-treatment techniques in the isolation and purification of monoclonal IgG antibody |
| title_fullStr | The performance of different pre-treatment techniques in the isolation and purification of monoclonal IgG antibody |
| title_full_unstemmed | The performance of different pre-treatment techniques in the isolation and purification of monoclonal IgG antibody |
| title_short | The performance of different pre-treatment techniques in the isolation and purification of monoclonal IgG antibody |
| title_sort | performance of different pre-treatment techniques in the isolation and purification of monoclonal igg antibody |
| topic | QR Microbiology |
| url | http://eprints.utm.my/178/ http://eprints.utm.my/178/1/MelissaLohPeiShiana2006_Theperformanceofdifferentpre-treatment.pdf |