Bioproduction of lipopeptides by dye decolourising bacteria and its roles in textile dye decolourisation

Textile dyes are recalcitrant molecules and contain a high level of chemicals and colour which poses a serious challenge to surrounding environments. Therefore, this study aims to determine the ability of isolated bacteria in producing a biosurfactant and investigate their synergistic action on deco...

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Main Author: Mohd Nor, Farrah Husna
Format: Thesis
Language:English
English
English
Published: 2022
Subjects:
Online Access:http://eprints.uthm.edu.my/8260/
http://eprints.uthm.edu.my/8260/2/24p%20FARHAH%20HUSNA%20MOHD%20NOR.pdf
http://eprints.uthm.edu.my/8260/1/FARHAH%20HUSNA%20MOHD%20NOR%20COPYRIGHT%20DECLARATION.pdf
http://eprints.uthm.edu.my/8260/3/FARHAH%20HUSNA%20MOHD%20NOR%20WATERMARK.pdf
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author Mohd Nor, Farrah Husna
author_facet Mohd Nor, Farrah Husna
author_sort Mohd Nor, Farrah Husna
building UTHM Institutional Repository
collection Online Access
description Textile dyes are recalcitrant molecules and contain a high level of chemicals and colour which poses a serious challenge to surrounding environments. Therefore, this study aims to determine the ability of isolated bacteria in producing a biosurfactant and investigate their synergistic action on decolourisation of textile dyes by the combination of isolated bacteria and biosurfactant. An effective dye degrading bacteria and biosurfactant-producers, Bacillus cereus KHl and Kurthia gibsonii KH2, were isolated from textile industrial effluents and were identified and screened for biosurfactant production using haemolytic activity, oil spreading technique, drop collapse test and emulsification index. The optimisation for growth of bacteria for biosurfactant production also was measured based on effect of molasses concentration, pH, salinity and temperature. Fourier Transform Infrared Spectrum (FTIR) and Thin Layer Chromatography (TLC) analyses were carried out to detect the type of biosurfactant. The effect of different physicochemical parameters on textile effluent decolourisation was assessed within 24 hours. As results, both isolated bacteria showed
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spelling uthm-82602023-02-07T03:37:55Z http://eprints.uthm.edu.my/8260/ Bioproduction of lipopeptides by dye decolourising bacteria and its roles in textile dye decolourisation Mohd Nor, Farrah Husna T Technology (General) Textile dyes are recalcitrant molecules and contain a high level of chemicals and colour which poses a serious challenge to surrounding environments. Therefore, this study aims to determine the ability of isolated bacteria in producing a biosurfactant and investigate their synergistic action on decolourisation of textile dyes by the combination of isolated bacteria and biosurfactant. An effective dye degrading bacteria and biosurfactant-producers, Bacillus cereus KHl and Kurthia gibsonii KH2, were isolated from textile industrial effluents and were identified and screened for biosurfactant production using haemolytic activity, oil spreading technique, drop collapse test and emulsification index. The optimisation for growth of bacteria for biosurfactant production also was measured based on effect of molasses concentration, pH, salinity and temperature. Fourier Transform Infrared Spectrum (FTIR) and Thin Layer Chromatography (TLC) analyses were carried out to detect the type of biosurfactant. The effect of different physicochemical parameters on textile effluent decolourisation was assessed within 24 hours. As results, both isolated bacteria showed 2022-01 Thesis NonPeerReviewed text en http://eprints.uthm.edu.my/8260/2/24p%20FARHAH%20HUSNA%20MOHD%20NOR.pdf text en http://eprints.uthm.edu.my/8260/1/FARHAH%20HUSNA%20MOHD%20NOR%20COPYRIGHT%20DECLARATION.pdf text en http://eprints.uthm.edu.my/8260/3/FARHAH%20HUSNA%20MOHD%20NOR%20WATERMARK.pdf Mohd Nor, Farrah Husna (2022) Bioproduction of lipopeptides by dye decolourising bacteria and its roles in textile dye decolourisation. Masters thesis, Universiti Tun Hussein Onn Malaysia.
spellingShingle T Technology (General)
Mohd Nor, Farrah Husna
Bioproduction of lipopeptides by dye decolourising bacteria and its roles in textile dye decolourisation
title Bioproduction of lipopeptides by dye decolourising bacteria and its roles in textile dye decolourisation
title_full Bioproduction of lipopeptides by dye decolourising bacteria and its roles in textile dye decolourisation
title_fullStr Bioproduction of lipopeptides by dye decolourising bacteria and its roles in textile dye decolourisation
title_full_unstemmed Bioproduction of lipopeptides by dye decolourising bacteria and its roles in textile dye decolourisation
title_short Bioproduction of lipopeptides by dye decolourising bacteria and its roles in textile dye decolourisation
title_sort bioproduction of lipopeptides by dye decolourising bacteria and its roles in textile dye decolourisation
topic T Technology (General)
url http://eprints.uthm.edu.my/8260/
http://eprints.uthm.edu.my/8260/2/24p%20FARHAH%20HUSNA%20MOHD%20NOR.pdf
http://eprints.uthm.edu.my/8260/1/FARHAH%20HUSNA%20MOHD%20NOR%20COPYRIGHT%20DECLARATION.pdf
http://eprints.uthm.edu.my/8260/3/FARHAH%20HUSNA%20MOHD%20NOR%20WATERMARK.pdf