Phytochemical analysis and in-vitro antiurolithiatic properties of selected Malaysian herbs

The aim of this study was to investigate the phytochemical content of selected Malaysian herbs and their potential antiurolithiatic effects using in-vitro method. The herbs involved are Ceiba pentandra, Cymbopogon citratus, Euphorbia hirta, Melastoma malabathricum and Ortosiphon stamineus. Aqueou...

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Main Authors: Wan Marzuki Rashidi, Wan Nur Ain Syukriah, Muhammad, Norhayati, Sairi, Nur Halimah, Abdul Rahim, Nur Fazira, A. Talip, Balkis, Abdullah, Norazlin, Abu Bakar, Mohd Fadzelly
Format: Article
Language:English
Published: Penerbit Akademia Baru 2019
Subjects:
Online Access:http://eprints.uthm.edu.my/813/
http://eprints.uthm.edu.my/813/1/DNJ9787_9ac7ec6f6d09160f75d9006881f41800.pdf
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author Wan Marzuki Rashidi, Wan Nur Ain Syukriah
Muhammad, Norhayati
Sairi, Nur Halimah
Abdul Rahim, Nur Fazira
A. Talip, Balkis
Abdullah, Norazlin
Abu Bakar, Mohd Fadzelly
author_facet Wan Marzuki Rashidi, Wan Nur Ain Syukriah
Muhammad, Norhayati
Sairi, Nur Halimah
Abdul Rahim, Nur Fazira
A. Talip, Balkis
Abdullah, Norazlin
Abu Bakar, Mohd Fadzelly
author_sort Wan Marzuki Rashidi, Wan Nur Ain Syukriah
building UTHM Institutional Repository
collection Online Access
description The aim of this study was to investigate the phytochemical content of selected Malaysian herbs and their potential antiurolithiatic effects using in-vitro method. The herbs involved are Ceiba pentandra, Cymbopogon citratus, Euphorbia hirta, Melastoma malabathricum and Ortosiphon stamineus. Aqueous extracts of each herbs were prepared through decoction while Standard drugs Cystone, was used as positive control in comparison. Qualitative analysis was carried out to detect phytochemical presence and nucleation assay to investigate their inhibition effects towards calcium oxalate crystallization urolithiasis in vitro. Based on results, the same trends were observed between phytochemical content and inhibition rate of calcium oxalate crystallization. O. stamineus extract (73.48%) which showed the highest inhibition rate hold the most phytochemical content. while the lowest inhibition rate was occupied by C. citratus extract (45.45%) with the least phytochemical content. The high amount of phytochemicals particularly saponin followed by steroid and terpenoid in O. stamineus extract might contributes to the high inhibition activities of calcium oxalate crystallization as compared to low amount of phytochemicals observed in C. citratus extract. It can be concluded that O. stamineus possesses highest inhibition percentage against calcium oxalate which could be attributed to its saponins, tannins, steroid and terpenoid content.
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spelling uthm-8132021-10-17T07:01:48Z http://eprints.uthm.edu.my/813/ Phytochemical analysis and in-vitro antiurolithiatic properties of selected Malaysian herbs Wan Marzuki Rashidi, Wan Nur Ain Syukriah Muhammad, Norhayati Sairi, Nur Halimah Abdul Rahim, Nur Fazira A. Talip, Balkis Abdullah, Norazlin Abu Bakar, Mohd Fadzelly QH Natural history The aim of this study was to investigate the phytochemical content of selected Malaysian herbs and their potential antiurolithiatic effects using in-vitro method. The herbs involved are Ceiba pentandra, Cymbopogon citratus, Euphorbia hirta, Melastoma malabathricum and Ortosiphon stamineus. Aqueous extracts of each herbs were prepared through decoction while Standard drugs Cystone, was used as positive control in comparison. Qualitative analysis was carried out to detect phytochemical presence and nucleation assay to investigate their inhibition effects towards calcium oxalate crystallization urolithiasis in vitro. Based on results, the same trends were observed between phytochemical content and inhibition rate of calcium oxalate crystallization. O. stamineus extract (73.48%) which showed the highest inhibition rate hold the most phytochemical content. while the lowest inhibition rate was occupied by C. citratus extract (45.45%) with the least phytochemical content. The high amount of phytochemicals particularly saponin followed by steroid and terpenoid in O. stamineus extract might contributes to the high inhibition activities of calcium oxalate crystallization as compared to low amount of phytochemicals observed in C. citratus extract. It can be concluded that O. stamineus possesses highest inhibition percentage against calcium oxalate which could be attributed to its saponins, tannins, steroid and terpenoid content. Penerbit Akademia Baru 2019 Article PeerReviewed text en http://eprints.uthm.edu.my/813/1/DNJ9787_9ac7ec6f6d09160f75d9006881f41800.pdf Wan Marzuki Rashidi, Wan Nur Ain Syukriah and Muhammad, Norhayati and Sairi, Nur Halimah and Abdul Rahim, Nur Fazira and A. Talip, Balkis and Abdullah, Norazlin and Abu Bakar, Mohd Fadzelly (2019) Phytochemical analysis and in-vitro antiurolithiatic properties of selected Malaysian herbs. Journal of Advanced Research in Fluid Mechanics and Thermal Sciences, 64 (1). pp. 152-159. ISSN 2289-7879
spellingShingle QH Natural history
Wan Marzuki Rashidi, Wan Nur Ain Syukriah
Muhammad, Norhayati
Sairi, Nur Halimah
Abdul Rahim, Nur Fazira
A. Talip, Balkis
Abdullah, Norazlin
Abu Bakar, Mohd Fadzelly
Phytochemical analysis and in-vitro antiurolithiatic properties of selected Malaysian herbs
title Phytochemical analysis and in-vitro antiurolithiatic properties of selected Malaysian herbs
title_full Phytochemical analysis and in-vitro antiurolithiatic properties of selected Malaysian herbs
title_fullStr Phytochemical analysis and in-vitro antiurolithiatic properties of selected Malaysian herbs
title_full_unstemmed Phytochemical analysis and in-vitro antiurolithiatic properties of selected Malaysian herbs
title_short Phytochemical analysis and in-vitro antiurolithiatic properties of selected Malaysian herbs
title_sort phytochemical analysis and in-vitro antiurolithiatic properties of selected malaysian herbs
topic QH Natural history
url http://eprints.uthm.edu.my/813/
http://eprints.uthm.edu.my/813/1/DNJ9787_9ac7ec6f6d09160f75d9006881f41800.pdf