Phylogeny of sea cucumber (echinodermata: holothuroidea) as inferred from 16s mitochondrial rRNa gene sequences
This study aimed to determine phylogenetic relationship between and among selected species of sea cucumbers (Echinodermata: Holothuroidea) using 16S mitochondrial ribosomal RNA (rRNA) gene. Phylogenetic analyses of 37 partial sequences of 16S mitochondrial rRNA gene using three main methods name...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaya
2010
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| Online Access: | http://eprints.uthm.edu.my/7860/ http://eprints.uthm.edu.my/7860/1/J6747_e3fd85144ac89359e128f71f040727ac.pdf |
| _version_ | 1848889227169759232 |
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| author | Kamarudin, Kamarul Rahim Hashim, Ridzwan Usup, Gires |
| author_facet | Kamarudin, Kamarul Rahim Hashim, Ridzwan Usup, Gires |
| author_sort | Kamarudin, Kamarul Rahim |
| building | UTHM Institutional Repository |
| collection | Online Access |
| description | This study aimed to determine phylogenetic relationship between and among selected species of sea cucumbers
(Echinodermata: Holothuroidea) using 16S mitochondrial ribosomal RNA (rRNA) gene. Phylogenetic analyses of 37
partial sequences of 16S mitochondrial rRNA gene using three main methods namely neighbour joining (NJ), maximum
parsimony (MP) and maximum likelihood (ML) showed the presence of five main genera of sea cucumbers: Molpadia
from order Molpadiida and four genera of order Aspidochirotida namely Holothuria, Stichopus, Bohadschia and
Actinopyga. All of the 17 species obtained from Malaysia distributed among the main genera except within Actinopyga.
Interestingly, Holothuria excellens was out of Holothuria group causing Holothuria to be paraphyletic. High bootstrap
value and consistent clustering made Molpadia, Stichopus, Bohadschia and Actinopyga monophyletic. The relationship
of Actinopyga with the other genera was unclarified and Stichopus was sister to Molpadia. The latter finding caused
the resolution at order level unclear. The pairwise genetic distance calculated using Kimura 2-parameter model further
supported and verified findings from the phylogenetic trees. Further studies with more samples and different mitochondrial
DNA genes need to be done to get a better view and verification on the molecular phylogeny of sea cucumbers |
| first_indexed | 2025-11-15T20:22:49Z |
| format | Article |
| id | uthm-7860 |
| institution | Universiti Tun Hussein Onn Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:22:49Z |
| publishDate | 2010 |
| publisher | Penerbit Universiti Kebangsaan Malaya |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uthm-78602022-10-17T06:17:25Z http://eprints.uthm.edu.my/7860/ Phylogeny of sea cucumber (echinodermata: holothuroidea) as inferred from 16s mitochondrial rRNa gene sequences Kamarudin, Kamarul Rahim Hashim, Ridzwan Usup, Gires T Technology (General) This study aimed to determine phylogenetic relationship between and among selected species of sea cucumbers (Echinodermata: Holothuroidea) using 16S mitochondrial ribosomal RNA (rRNA) gene. Phylogenetic analyses of 37 partial sequences of 16S mitochondrial rRNA gene using three main methods namely neighbour joining (NJ), maximum parsimony (MP) and maximum likelihood (ML) showed the presence of five main genera of sea cucumbers: Molpadia from order Molpadiida and four genera of order Aspidochirotida namely Holothuria, Stichopus, Bohadschia and Actinopyga. All of the 17 species obtained from Malaysia distributed among the main genera except within Actinopyga. Interestingly, Holothuria excellens was out of Holothuria group causing Holothuria to be paraphyletic. High bootstrap value and consistent clustering made Molpadia, Stichopus, Bohadschia and Actinopyga monophyletic. The relationship of Actinopyga with the other genera was unclarified and Stichopus was sister to Molpadia. The latter finding caused the resolution at order level unclear. The pairwise genetic distance calculated using Kimura 2-parameter model further supported and verified findings from the phylogenetic trees. Further studies with more samples and different mitochondrial DNA genes need to be done to get a better view and verification on the molecular phylogeny of sea cucumbers Penerbit Universiti Kebangsaan Malaya 2010 Article PeerReviewed text en http://eprints.uthm.edu.my/7860/1/J6747_e3fd85144ac89359e128f71f040727ac.pdf Kamarudin, Kamarul Rahim and Hashim, Ridzwan and Usup, Gires (2010) Phylogeny of sea cucumber (echinodermata: holothuroidea) as inferred from 16s mitochondrial rRNa gene sequences. Sains Malaysiana, 39 (2). pp. 209-218. |
| spellingShingle | T Technology (General) Kamarudin, Kamarul Rahim Hashim, Ridzwan Usup, Gires Phylogeny of sea cucumber (echinodermata: holothuroidea) as inferred from 16s mitochondrial rRNa gene sequences |
| title | Phylogeny of sea cucumber (echinodermata: holothuroidea) as inferred from 16s mitochondrial rRNa gene sequences |
| title_full | Phylogeny of sea cucumber (echinodermata: holothuroidea) as inferred from 16s mitochondrial rRNa gene sequences |
| title_fullStr | Phylogeny of sea cucumber (echinodermata: holothuroidea) as inferred from 16s mitochondrial rRNa gene sequences |
| title_full_unstemmed | Phylogeny of sea cucumber (echinodermata: holothuroidea) as inferred from 16s mitochondrial rRNa gene sequences |
| title_short | Phylogeny of sea cucumber (echinodermata: holothuroidea) as inferred from 16s mitochondrial rRNa gene sequences |
| title_sort | phylogeny of sea cucumber (echinodermata: holothuroidea) as inferred from 16s mitochondrial rrna gene sequences |
| topic | T Technology (General) |
| url | http://eprints.uthm.edu.my/7860/ http://eprints.uthm.edu.my/7860/1/J6747_e3fd85144ac89359e128f71f040727ac.pdf |