Siam-weeb based gelatin electrospun scaffolds

Siam weed (Chromolaena odorata) is a traditional herb used to soothe burns and scars and potentially improve dressing and tissue-engineered construct healing ability. The use of Siam weed is mainly in the form of extract. The state of liquid extract limits the use of Siam weed compared to the...

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Main Authors: Mohamed, Nor Shamsiah, Jun Ham, Jun Ham, Ab-Rahim, Sharaniza, Cheng, Yee Low, Ching, Theng Koh
Format: Article
Language:English
Published: Microscopy Society of Malaysia (MSM) 2022
Subjects:
Online Access:http://eprints.uthm.edu.my/7720/
http://eprints.uthm.edu.my/7720/1/J14697_09a70dad05ec383e273be22e6000bdab.pdf
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author Mohamed, Nor Shamsiah
Jun Ham, Jun Ham
Ab-Rahim, Sharaniza
Cheng, Yee Low
Ching, Theng Koh
author_facet Mohamed, Nor Shamsiah
Jun Ham, Jun Ham
Ab-Rahim, Sharaniza
Cheng, Yee Low
Ching, Theng Koh
author_sort Mohamed, Nor Shamsiah
building UTHM Institutional Repository
collection Online Access
description Siam weed (Chromolaena odorata) is a traditional herb used to soothe burns and scars and potentially improve dressing and tissue-engineered construct healing ability. The use of Siam weed is mainly in the form of extract. The state of liquid extract limits the use of Siam weed compared to the state of gels and membrane. However, the development of the hybrid of Siam weed in gel and membrane form is lacking. In this study, Siam weed was harvested and made into aqua extracts. The biocompatibility of the aqua extract with various concentrations was tested using Cell Proliferation Assay. The aqua extract was then incorporated into the gelatin solution and spun into nanofibers using an electrospinning technique. The cell proliferation study shows maximum cell proliferation at the 25 μg/ml concentration. The scanning electron microscope images of the electrospun scaffolds show fibrous networks’ microstructures without beads. The concentration of the hybrid solution was found to affect the morphology of the nanofibers by having diameters in the range of 160 ± 90 to 250 ± 150 nm. The Siam-weed-based gelatin electrospun scaffolds provide a native-like microenvironment and potentially improve wound healing ability for biomedical application.
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publisher Microscopy Society of Malaysia (MSM)
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spelling uthm-77202022-10-05T07:02:16Z http://eprints.uthm.edu.my/7720/ Siam-weeb based gelatin electrospun scaffolds Mohamed, Nor Shamsiah Jun Ham, Jun Ham Ab-Rahim, Sharaniza Cheng, Yee Low Ching, Theng Koh T11.95-12.5 Industrial directories Siam weed (Chromolaena odorata) is a traditional herb used to soothe burns and scars and potentially improve dressing and tissue-engineered construct healing ability. The use of Siam weed is mainly in the form of extract. The state of liquid extract limits the use of Siam weed compared to the state of gels and membrane. However, the development of the hybrid of Siam weed in gel and membrane form is lacking. In this study, Siam weed was harvested and made into aqua extracts. The biocompatibility of the aqua extract with various concentrations was tested using Cell Proliferation Assay. The aqua extract was then incorporated into the gelatin solution and spun into nanofibers using an electrospinning technique. The cell proliferation study shows maximum cell proliferation at the 25 μg/ml concentration. The scanning electron microscope images of the electrospun scaffolds show fibrous networks’ microstructures without beads. The concentration of the hybrid solution was found to affect the morphology of the nanofibers by having diameters in the range of 160 ± 90 to 250 ± 150 nm. The Siam-weed-based gelatin electrospun scaffolds provide a native-like microenvironment and potentially improve wound healing ability for biomedical application. Microscopy Society of Malaysia (MSM) 2022 Article PeerReviewed text en http://eprints.uthm.edu.my/7720/1/J14697_09a70dad05ec383e273be22e6000bdab.pdf Mohamed, Nor Shamsiah and Jun Ham, Jun Ham and Ab-Rahim, Sharaniza and Cheng, Yee Low and Ching, Theng Koh (2022) Siam-weeb based gelatin electrospun scaffolds. Malaysian Journal of Microscopy, 18 (1). pp. 147-157. ISSN 1823-7010
spellingShingle T11.95-12.5 Industrial directories
Mohamed, Nor Shamsiah
Jun Ham, Jun Ham
Ab-Rahim, Sharaniza
Cheng, Yee Low
Ching, Theng Koh
Siam-weeb based gelatin electrospun scaffolds
title Siam-weeb based gelatin electrospun scaffolds
title_full Siam-weeb based gelatin electrospun scaffolds
title_fullStr Siam-weeb based gelatin electrospun scaffolds
title_full_unstemmed Siam-weeb based gelatin electrospun scaffolds
title_short Siam-weeb based gelatin electrospun scaffolds
title_sort siam-weeb based gelatin electrospun scaffolds
topic T11.95-12.5 Industrial directories
url http://eprints.uthm.edu.my/7720/
http://eprints.uthm.edu.my/7720/1/J14697_09a70dad05ec383e273be22e6000bdab.pdf