Siam-weeb based gelatin electrospun scaffolds
Siam weed (Chromolaena odorata) is a traditional herb used to soothe burns and scars and potentially improve dressing and tissue-engineered construct healing ability. The use of Siam weed is mainly in the form of extract. The state of liquid extract limits the use of Siam weed compared to the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Microscopy Society of Malaysia (MSM)
2022
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/7720/ http://eprints.uthm.edu.my/7720/1/J14697_09a70dad05ec383e273be22e6000bdab.pdf |
| Summary: | Siam weed (Chromolaena odorata) is a traditional herb used to soothe burns and
scars and potentially improve dressing and tissue-engineered construct healing ability. The use
of Siam weed is mainly in the form of extract. The state of liquid extract limits the use of Siam
weed compared to the state of gels and membrane. However, the development of the hybrid of
Siam weed in gel and membrane form is lacking. In this study, Siam weed was harvested and
made into aqua extracts. The biocompatibility of the aqua extract with various concentrations
was tested using Cell Proliferation Assay. The aqua extract was then incorporated into the
gelatin solution and spun into nanofibers using an electrospinning technique. The cell
proliferation study shows maximum cell proliferation at the 25 μg/ml concentration. The
scanning electron microscope images of the electrospun scaffolds show fibrous networks’
microstructures without beads. The concentration of the hybrid solution was found to affect the
morphology of the nanofibers by having diameters in the range of 160 ± 90 to 250 ± 150 nm.
The Siam-weed-based gelatin electrospun scaffolds provide a native-like microenvironment
and potentially improve wound healing ability for biomedical application. |
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