Effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 °C for 50 hours
Physical and chemical changes caused by oxidative stress in the spermatozoa membrane can reduce spermatozoa function and even lead to death. Cystamine (NH2-CH2-CH2-SH, β-mercaptoethylamine) is a natural substance that modulates the endocrine and metabolic status of animals. This substance has an...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Razi Vaccine & Serum Research Institute
2021
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/7262/ http://eprints.uthm.edu.my/7262/1/J14281_ac4d1a1afe89fa80dc800bd85fa05af2.pdf |
| _version_ | 1848889047195320320 |
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| author | J., Jumintono S., Alkubaisy D., Yánez Silva K., Singh A., Turki Jali S., Mutia Syarifah Y., Fakri Mustafa I., Mikolaychik L., Morozova M., Derkho |
| author_facet | J., Jumintono S., Alkubaisy D., Yánez Silva K., Singh A., Turki Jali S., Mutia Syarifah Y., Fakri Mustafa I., Mikolaychik L., Morozova M., Derkho |
| author_sort | J., Jumintono |
| building | UTHM Institutional Repository |
| collection | Online Access |
| description | Physical and chemical changes caused by oxidative stress in the spermatozoa membrane can reduce
spermatozoa function and even lead to death. Cystamine (NH2-CH2-CH2-SH, β-mercaptoethylamine) is a
natural substance that modulates the endocrine and metabolic status of animals. This substance has antioxidant
and anti-apoptotic effects by inducing intracellular cysteine accumulation. Cystamine is used to treat many
diseases despite its many side effects. Sheep semen is sensitive to the stressful condition of chilling storage,
which restricts semen storage for artificial insemination in commercial herds. The effect of cystamine on
spermatogenesis is not yet fully understood. The present study aimed to investigate the effect of cysteamine
addition to the sheep sperm extender during cooling storage on semen quality parameters. Sperm samples were
collected from six Edilbayevskaya rams (2 and 3 years old, 70-85 kg). The samples were diluted by extender
and supplemented with different concentrations of cysteamine (0, 1, 2, 5, and 10 mM) and cooled to 4ºC for 50
h. Motility parameters, membrane integrity, viability, lipid peroxidation, and mitochondrial activity of cooled
semen were evaluated at 0, 25, and 50 h of cooling storage. Although cysteamine failed to affect semen quality
at start time (0 hrs), extender supplementation with cysteamine improved sperm total motility, progressive
motility, and mitochondrial membrane potential during storage periods (P≤0.01). Moreover, using 1 and 2 mM
cysteamine functionally and viably improved (P≤0.01) sperm membrane compared to other treatments.
Antioxidant potential (AOP), lipid peroxidation (LPO), and total glutathione (tGSH) (except AOP at 50 h) were
significantly different after semen storage at 4 °C. Therefore, levels of AOP and tGSH were significantly
increased by using cysteamine. Cysteamine supplementation (1 and 2 mM cysteamine) leads to lower levels of
LPO (p<0.01) at 0, 25, and 50 h. Therefore, finding and using the best concentrations of cysteamine in a cooling
extender could be effective in saving sheep semen against damages of the cooling storage process. |
| first_indexed | 2025-11-15T20:19:58Z |
| format | Article |
| id | uthm-7262 |
| institution | Universiti Tun Hussein Onn Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:19:58Z |
| publishDate | 2021 |
| publisher | Razi Vaccine & Serum Research Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uthm-72622022-07-12T01:48:11Z http://eprints.uthm.edu.my/7262/ Effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 °C for 50 hours J., Jumintono S., Alkubaisy D., Yánez Silva K., Singh A., Turki Jali S., Mutia Syarifah Y., Fakri Mustafa I., Mikolaychik L., Morozova M., Derkho T Technology (General) Physical and chemical changes caused by oxidative stress in the spermatozoa membrane can reduce spermatozoa function and even lead to death. Cystamine (NH2-CH2-CH2-SH, β-mercaptoethylamine) is a natural substance that modulates the endocrine and metabolic status of animals. This substance has antioxidant and anti-apoptotic effects by inducing intracellular cysteine accumulation. Cystamine is used to treat many diseases despite its many side effects. Sheep semen is sensitive to the stressful condition of chilling storage, which restricts semen storage for artificial insemination in commercial herds. The effect of cystamine on spermatogenesis is not yet fully understood. The present study aimed to investigate the effect of cysteamine addition to the sheep sperm extender during cooling storage on semen quality parameters. Sperm samples were collected from six Edilbayevskaya rams (2 and 3 years old, 70-85 kg). The samples were diluted by extender and supplemented with different concentrations of cysteamine (0, 1, 2, 5, and 10 mM) and cooled to 4ºC for 50 h. Motility parameters, membrane integrity, viability, lipid peroxidation, and mitochondrial activity of cooled semen were evaluated at 0, 25, and 50 h of cooling storage. Although cysteamine failed to affect semen quality at start time (0 hrs), extender supplementation with cysteamine improved sperm total motility, progressive motility, and mitochondrial membrane potential during storage periods (P≤0.01). Moreover, using 1 and 2 mM cysteamine functionally and viably improved (P≤0.01) sperm membrane compared to other treatments. Antioxidant potential (AOP), lipid peroxidation (LPO), and total glutathione (tGSH) (except AOP at 50 h) were significantly different after semen storage at 4 °C. Therefore, levels of AOP and tGSH were significantly increased by using cysteamine. Cysteamine supplementation (1 and 2 mM cysteamine) leads to lower levels of LPO (p<0.01) at 0, 25, and 50 h. Therefore, finding and using the best concentrations of cysteamine in a cooling extender could be effective in saving sheep semen against damages of the cooling storage process. Razi Vaccine & Serum Research Institute 2021 Article PeerReviewed text en http://eprints.uthm.edu.my/7262/1/J14281_ac4d1a1afe89fa80dc800bd85fa05af2.pdf J., Jumintono and S., Alkubaisy and D., Yánez Silva and K., Singh and A., Turki Jali and S., Mutia Syarifah and Y., Fakri Mustafa and I., Mikolaychik and L., Morozova and M., Derkho (2021) Effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 °C for 50 hours. Archives of Razi Institute, 76 (4). pp. 1115-1123. https://doi.org/ 10.22092/ari.2021.355901.1735 |
| spellingShingle | T Technology (General) J., Jumintono S., Alkubaisy D., Yánez Silva K., Singh A., Turki Jali S., Mutia Syarifah Y., Fakri Mustafa I., Mikolaychik L., Morozova M., Derkho Effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 °C for 50 hours |
| title | Effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 °C for 50 hours |
| title_full | Effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 °C for 50 hours |
| title_fullStr | Effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 °C for 50 hours |
| title_full_unstemmed | Effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 °C for 50 hours |
| title_short | Effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 °C for 50 hours |
| title_sort | effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 °c for 50 hours |
| topic | T Technology (General) |
| url | http://eprints.uthm.edu.my/7262/ http://eprints.uthm.edu.my/7262/ http://eprints.uthm.edu.my/7262/1/J14281_ac4d1a1afe89fa80dc800bd85fa05af2.pdf |