Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering
This work reports the fabrication and characterizations of porous scaffold made up of polylactic acid (PLA) with the inclusion of pectin (1, 3, 5, 7, 9, 11wt%) for potential tissue engineering material. The composite scaffold was prepared using a facile method of freeze extraction. Based on the phys...
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| Format: | Article |
| Language: | English |
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DE GRUYTER
2020
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| Online Access: | http://eprints.uthm.edu.my/6819/ http://eprints.uthm.edu.my/6819/1/AJ%202020%20%28432%29.pdf |
| _version_ | 1848888919376003072 |
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| author | Anwar Hamzah, Mohd Syahir Abd Razak, Saiful Izwan Abdul Kadir, Mohammed Rafiq Mohd Bohari, Siti Pauliena Mat Nayan, Nadirul Hasraf Thangaraj Anand, Joseph Sahaya |
| author_facet | Anwar Hamzah, Mohd Syahir Abd Razak, Saiful Izwan Abdul Kadir, Mohammed Rafiq Mohd Bohari, Siti Pauliena Mat Nayan, Nadirul Hasraf Thangaraj Anand, Joseph Sahaya |
| author_sort | Anwar Hamzah, Mohd Syahir |
| building | UTHM Institutional Repository |
| collection | Online Access |
| description | This work reports the fabrication and characterizations of porous scaffold made up of polylactic acid (PLA) with the inclusion of pectin (1, 3, 5, 7, 9, 11wt%) for potential tissue engineering material. The composite scaffold was prepared using a facile method of freeze extraction. Based on the physical evaluations, the scaffold was suggested to be optimumat 5 wt%of pectin loading.Water contact angle of the scaffold was significantly reduced to 46.5o with the inclusion of 5 wt% of pectin. Morphological and topographic of the PLA scaffold revealed that the pectin induced more porous structure and its surface became rougher which was suitable for cell attachment and proliferation. In vitro studies of the PLA/pectin composite scaffold using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidelt (MTT) assay revealed good biocompatibility whereas Live-Dead kit assay resulted in 91%cell viability after 7 days of incubation. |
| first_indexed | 2025-11-15T20:17:56Z |
| format | Article |
| id | uthm-6819 |
| institution | Universiti Tun Hussein Onn Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:17:56Z |
| publishDate | 2020 |
| publisher | DE GRUYTER |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uthm-68192022-03-28T01:19:21Z http://eprints.uthm.edu.my/6819/ Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering Anwar Hamzah, Mohd Syahir Abd Razak, Saiful Izwan Abdul Kadir, Mohammed Rafiq Mohd Bohari, Siti Pauliena Mat Nayan, Nadirul Hasraf Thangaraj Anand, Joseph Sahaya TP Chemical technology This work reports the fabrication and characterizations of porous scaffold made up of polylactic acid (PLA) with the inclusion of pectin (1, 3, 5, 7, 9, 11wt%) for potential tissue engineering material. The composite scaffold was prepared using a facile method of freeze extraction. Based on the physical evaluations, the scaffold was suggested to be optimumat 5 wt%of pectin loading.Water contact angle of the scaffold was significantly reduced to 46.5o with the inclusion of 5 wt% of pectin. Morphological and topographic of the PLA scaffold revealed that the pectin induced more porous structure and its surface became rougher which was suitable for cell attachment and proliferation. In vitro studies of the PLA/pectin composite scaffold using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidelt (MTT) assay revealed good biocompatibility whereas Live-Dead kit assay resulted in 91%cell viability after 7 days of incubation. DE GRUYTER 2020 Article PeerReviewed text en http://eprints.uthm.edu.my/6819/1/AJ%202020%20%28432%29.pdf Anwar Hamzah, Mohd Syahir and Abd Razak, Saiful Izwan and Abdul Kadir, Mohammed Rafiq and Mohd Bohari, Siti Pauliena and Mat Nayan, Nadirul Hasraf and Thangaraj Anand, Joseph Sahaya (2020) Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering. JOURNAL OF POLYMER ENGINEERING, 40 (5). pp. 421-431. ISSN 21910340 https://doi.org/10.1515/polyeng-2019-0377 |
| spellingShingle | TP Chemical technology Anwar Hamzah, Mohd Syahir Abd Razak, Saiful Izwan Abdul Kadir, Mohammed Rafiq Mohd Bohari, Siti Pauliena Mat Nayan, Nadirul Hasraf Thangaraj Anand, Joseph Sahaya Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering |
| title | Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering |
| title_full | Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering |
| title_fullStr | Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering |
| title_full_unstemmed | Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering |
| title_short | Fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering |
| title_sort | fabrication and evaluation of polylactic acid/ pectin composite scaffold via freeze extraction for tissue engineering |
| topic | TP Chemical technology |
| url | http://eprints.uthm.edu.my/6819/ http://eprints.uthm.edu.my/6819/ http://eprints.uthm.edu.my/6819/1/AJ%202020%20%28432%29.pdf |