Enzymatic hydrolysis of thermally pre-treated chitin and antimicrobial activity of N,N′-diacetylchitobiose
Background: n,n′-diacetylchitobiose (glc n ac2) is known to be highly functional and offers a wide range of applications, especially as an antimicrobial agent. In this study, a thermal pre-treatment process using steam under pressure in an autoclave was employed to facilitate subsequent enzymatic hy...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/6136/ http://eprints.uthm.edu.my/6136/1/AJ%202020%20%28207%29.pdf |
| Summary: | Background: n,n′-diacetylchitobiose (glc n ac2) is known to be highly functional and offers a wide range of applications, especially as an antimicrobial agent. In this study, a thermal pre-treatment process using steam under pressure in an autoclave was employed to facilitate subsequent enzymatic hydrolysis of chitin with chitinase from streptomyces griseus. Results: pre-treatment of chitin with 0.05mol l−1 sodium acetate buffer (p h= 6.0) at 121 ∘c for 60 min, followed by enzymatic hydrolysis involving24 hincubation,were foundtobethebest conditions for producing glc n ac2. the glc n ac2 thus obtainedwas tested regarding its antimicrobial activity against gram-negative and gram-positive strains and showed minimum inhibitory concentrations at 5 and 10% (w/v) against escherichia coli k-12 and listeria monocytogenes 10403 s, respectively. Conclusions: the extent of swelling and crystallite size of chitin increased with pre-treatment residence time, and enhanced the rate of subsequent hydrolysis using chitinase. © 2019 society of chemical industry |
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