Isolation and identification of amylolytic bacteria from ragi
Ragi is a traditional dry starter culture frequently used in many fermented food products in Asian countries. Ragi contains starch that can be degraded by amylases provided by bacteria and other microorganisms during fermentation in producing fermented foods. This study was carried out to isolate ba...
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| Format: | Article |
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The Malaysian Society of Applied Biology
2018
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| Online Access: | http://eprints.uthm.edu.my/5801/ |
| _version_ | 1848888637071032320 |
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| author | Roslan, Rosazrina Mohamed Rehan, Maryam Kamarudin, Kamarul Rahim Mohd Noor, Hanina Faujan, Nur Huda Mat Radzi, Salina |
| author_facet | Roslan, Rosazrina Mohamed Rehan, Maryam Kamarudin, Kamarul Rahim Mohd Noor, Hanina Faujan, Nur Huda Mat Radzi, Salina |
| author_sort | Roslan, Rosazrina |
| building | UTHM Institutional Repository |
| collection | Online Access |
| description | Ragi is a traditional dry starter culture frequently used in many fermented food products in Asian countries. Ragi contains starch that can be degraded by amylases provided by bacteria and other microorganisms during fermentation in producing fermented foods. This study was carried out to isolate bacteria from ragi and to screen for their ability to hydrolyse starch. Seven bacterial colonies were isolated from ragi, with three samples showing starch hydrolysis activity. Two of the starchdegrading bacterial isolates were identified using partial 16S rRNA gene sequencing as Bacillus licheniformis with 97% and 98% similarity, and one sample identified as a lactic acid bacteria, Enterococcus faecium (98%). Isolation and identification of these bacteria from ragi can provide a promising source of amylase that can be further studied and manipulated for the development of starter culture and to improve the quality of traditional ragi-based fermented food products. |
| first_indexed | 2025-11-15T20:13:27Z |
| format | Article |
| id | uthm-5801 |
| institution | Universiti Tun Hussein Onn Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T20:13:27Z |
| publishDate | 2018 |
| publisher | The Malaysian Society of Applied Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uthm-58012022-01-23T08:12:19Z http://eprints.uthm.edu.my/5801/ Isolation and identification of amylolytic bacteria from ragi Roslan, Rosazrina Mohamed Rehan, Maryam Kamarudin, Kamarul Rahim Mohd Noor, Hanina Faujan, Nur Huda Mat Radzi, Salina TP Chemical technology Ragi is a traditional dry starter culture frequently used in many fermented food products in Asian countries. Ragi contains starch that can be degraded by amylases provided by bacteria and other microorganisms during fermentation in producing fermented foods. This study was carried out to isolate bacteria from ragi and to screen for their ability to hydrolyse starch. Seven bacterial colonies were isolated from ragi, with three samples showing starch hydrolysis activity. Two of the starchdegrading bacterial isolates were identified using partial 16S rRNA gene sequencing as Bacillus licheniformis with 97% and 98% similarity, and one sample identified as a lactic acid bacteria, Enterococcus faecium (98%). Isolation and identification of these bacteria from ragi can provide a promising source of amylase that can be further studied and manipulated for the development of starter culture and to improve the quality of traditional ragi-based fermented food products. The Malaysian Society of Applied Biology 2018 Article PeerReviewed Roslan, Rosazrina and Mohamed Rehan, Maryam and Kamarudin, Kamarul Rahim and Mohd Noor, Hanina and Faujan, Nur Huda and Mat Radzi, Salina (2018) Isolation and identification of amylolytic bacteria from ragi. Malaysian Applied Biology, 47 (2). pp. 83-88. ISSN 2462-151X |
| spellingShingle | TP Chemical technology Roslan, Rosazrina Mohamed Rehan, Maryam Kamarudin, Kamarul Rahim Mohd Noor, Hanina Faujan, Nur Huda Mat Radzi, Salina Isolation and identification of amylolytic bacteria from ragi |
| title | Isolation and identification of amylolytic bacteria from ragi |
| title_full | Isolation and identification of amylolytic bacteria from ragi |
| title_fullStr | Isolation and identification of amylolytic bacteria from ragi |
| title_full_unstemmed | Isolation and identification of amylolytic bacteria from ragi |
| title_short | Isolation and identification of amylolytic bacteria from ragi |
| title_sort | isolation and identification of amylolytic bacteria from ragi |
| topic | TP Chemical technology |
| url | http://eprints.uthm.edu.my/5801/ |