Phenotypic identification of Penicillium spp. isolated from clinical wastes based on microstructure characteristics

Aims: The present study aimed to recognize the microstructure of conidiophores and spores of Penicillium spp. which were isolated from clinical wastes. Methodology and results: The isolates of Penicillium spp. were obtained from the solid clinical wastes on V8A medium and purified by single spore me...

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Main Authors: Ali Noman, Efaq, Al-Gheethi, Adel Ali, Nik Ab. Rahman, Nik Norulaini, Hideyuki, Nagao, Haji A Talip, Balkis, Radin Mohamed, Radin Maya Saphira, Ab. Kadir, Mohd. Omar
Format: Article
Language:English
Published: Malaysian Society for Microbiology 2018
Subjects:
Online Access:http://eprints.uthm.edu.my/5676/
http://eprints.uthm.edu.my/5676/1/AJ%202018%20%28294%29.pdf
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author Ali Noman, Efaq
Al-Gheethi, Adel Ali
Nik Ab. Rahman, Nik Norulaini
Hideyuki, Nagao
Haji A Talip, Balkis
Radin Mohamed, Radin Maya Saphira
Ab. Kadir, Mohd. Omar
author_facet Ali Noman, Efaq
Al-Gheethi, Adel Ali
Nik Ab. Rahman, Nik Norulaini
Hideyuki, Nagao
Haji A Talip, Balkis
Radin Mohamed, Radin Maya Saphira
Ab. Kadir, Mohd. Omar
author_sort Ali Noman, Efaq
building UTHM Institutional Repository
collection Online Access
description Aims: The present study aimed to recognize the microstructure of conidiophores and spores of Penicillium spp. which were isolated from clinical wastes. Methodology and results: The isolates of Penicillium spp. were obtained from the solid clinical wastes on V8A medium and purified by single spore method. The culture characteristics were described in five culture media included; Czapek Yeast Extract Agar (CYA); Malt Extract Agar (MEA), Potato Dextrose Agar (PDA), Sabouraud Dextrose Agar (SDA) and Czapek-Dox Agar (CZ) while the conidiophores and spores were described using light and Scanning Electronic Microscope (SEM). Penicillium spp. observed some differences in their culture characteristics. Among 11 Penicillium species isolated in this study and identified based on culture and microscope morphology. Five species including P. simplicissium, P. waksmanii, P. corylophilum and P. decumbens as well as one species identified as T. wortmannii were described in detail using SEM. Conclusion, significance and impact of study: The study revealed that the microstructure of the fungal spores and conidiophores play an important role in the taxonomy of fungi species based on the phenotypic method.
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spelling uthm-56762022-01-20T04:30:55Z http://eprints.uthm.edu.my/5676/ Phenotypic identification of Penicillium spp. isolated from clinical wastes based on microstructure characteristics Ali Noman, Efaq Al-Gheethi, Adel Ali Nik Ab. Rahman, Nik Norulaini Hideyuki, Nagao Haji A Talip, Balkis Radin Mohamed, Radin Maya Saphira Ab. Kadir, Mohd. Omar R855-855.5 Medical technology Aims: The present study aimed to recognize the microstructure of conidiophores and spores of Penicillium spp. which were isolated from clinical wastes. Methodology and results: The isolates of Penicillium spp. were obtained from the solid clinical wastes on V8A medium and purified by single spore method. The culture characteristics were described in five culture media included; Czapek Yeast Extract Agar (CYA); Malt Extract Agar (MEA), Potato Dextrose Agar (PDA), Sabouraud Dextrose Agar (SDA) and Czapek-Dox Agar (CZ) while the conidiophores and spores were described using light and Scanning Electronic Microscope (SEM). Penicillium spp. observed some differences in their culture characteristics. Among 11 Penicillium species isolated in this study and identified based on culture and microscope morphology. Five species including P. simplicissium, P. waksmanii, P. corylophilum and P. decumbens as well as one species identified as T. wortmannii were described in detail using SEM. Conclusion, significance and impact of study: The study revealed that the microstructure of the fungal spores and conidiophores play an important role in the taxonomy of fungi species based on the phenotypic method. Malaysian Society for Microbiology 2018 Article PeerReviewed text en http://eprints.uthm.edu.my/5676/1/AJ%202018%20%28294%29.pdf Ali Noman, Efaq and Al-Gheethi, Adel Ali and Nik Ab. Rahman, Nik Norulaini and Hideyuki, Nagao and Haji A Talip, Balkis and Radin Mohamed, Radin Maya Saphira and Ab. Kadir, Mohd. Omar (2018) Phenotypic identification of Penicillium spp. isolated from clinical wastes based on microstructure characteristics. Malaysian Journal of Microbiology, 14 (2). pp. 88-95. ISSN 1823-8262
spellingShingle R855-855.5 Medical technology
Ali Noman, Efaq
Al-Gheethi, Adel Ali
Nik Ab. Rahman, Nik Norulaini
Hideyuki, Nagao
Haji A Talip, Balkis
Radin Mohamed, Radin Maya Saphira
Ab. Kadir, Mohd. Omar
Phenotypic identification of Penicillium spp. isolated from clinical wastes based on microstructure characteristics
title Phenotypic identification of Penicillium spp. isolated from clinical wastes based on microstructure characteristics
title_full Phenotypic identification of Penicillium spp. isolated from clinical wastes based on microstructure characteristics
title_fullStr Phenotypic identification of Penicillium spp. isolated from clinical wastes based on microstructure characteristics
title_full_unstemmed Phenotypic identification of Penicillium spp. isolated from clinical wastes based on microstructure characteristics
title_short Phenotypic identification of Penicillium spp. isolated from clinical wastes based on microstructure characteristics
title_sort phenotypic identification of penicillium spp. isolated from clinical wastes based on microstructure characteristics
topic R855-855.5 Medical technology
url http://eprints.uthm.edu.my/5676/
http://eprints.uthm.edu.my/5676/1/AJ%202018%20%28294%29.pdf