High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis
Advancement in industrial biotechnology offers potential opportunities for economic utilization of agro-industrial biomass such as kenaf (Hibiscus cannabinus) for the production of value-added products. This work was conducted to evaluate an enzymatic hydrolysis of kenaf stem (that has been pretreat...
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| Format: | Article |
| Language: | English |
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Elsevier
2016
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| Online Access: | http://eprints.uthm.edu.my/5266/ http://eprints.uthm.edu.my/5266/1/AJ%202016%20%2874%29.pdf |
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| author | Wan Azelee, Nur Izyan Md Jahim, Jamaliah Ismail, Ahmad Fauzi Mohamad Fuzi, Siti Fatimah Zaharah A. Rahman, Roshanida Md Illias, Rosli |
| author_facet | Wan Azelee, Nur Izyan Md Jahim, Jamaliah Ismail, Ahmad Fauzi Mohamad Fuzi, Siti Fatimah Zaharah A. Rahman, Roshanida Md Illias, Rosli |
| author_sort | Wan Azelee, Nur Izyan |
| building | UTHM Institutional Repository |
| collection | Online Access |
| description | Advancement in industrial biotechnology offers potential opportunities for economic utilization of agro-industrial biomass such as kenaf (Hibiscus cannabinus) for the production of value-added products. This work was conducted to evaluate an enzymatic hydrolysis of kenaf stem (that has been pretreated) for xylooligosaccharides (XOS) production. Pretreated kenaf stems were subjected to two enzymatic hydrolysis approaches which are single xylanase (Xyn2) and a mixture of xylanase:arabinofuranosidase (Xyn2:AnabfA). Central Composite Design was used to optimize the enzymatic hydrolysis conditions on the pretreated kenaf stem to achieve maximum hemicellulose conversion. Under the optimized hydrolysis conditions, hemicellulose conversion (95.03%) with the highest XOS yield of 351.46 mg/g was achieved at 40 °C (pH 4.0) after 48 h incubation with Xyn2:AnabfA ratio of 400 U:10 U. Xylobiose (135.42 mg/g) is the main XOS product with xylotriose (102.04 mg/g) being the second highest. The high production of XOS yield from the pretreated kenaf stem demonstrated the biotechnological potential of the kenaf in the future. |
| first_indexed | 2025-11-15T20:11:23Z |
| format | Article |
| id | uthm-5266 |
| institution | Universiti Tun Hussein Onn Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:11:23Z |
| publishDate | 2016 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uthm-52662022-01-06T08:19:59Z http://eprints.uthm.edu.my/5266/ High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis Wan Azelee, Nur Izyan Md Jahim, Jamaliah Ismail, Ahmad Fauzi Mohamad Fuzi, Siti Fatimah Zaharah A. Rahman, Roshanida Md Illias, Rosli QD Chemistry Advancement in industrial biotechnology offers potential opportunities for economic utilization of agro-industrial biomass such as kenaf (Hibiscus cannabinus) for the production of value-added products. This work was conducted to evaluate an enzymatic hydrolysis of kenaf stem (that has been pretreated) for xylooligosaccharides (XOS) production. Pretreated kenaf stems were subjected to two enzymatic hydrolysis approaches which are single xylanase (Xyn2) and a mixture of xylanase:arabinofuranosidase (Xyn2:AnabfA). Central Composite Design was used to optimize the enzymatic hydrolysis conditions on the pretreated kenaf stem to achieve maximum hemicellulose conversion. Under the optimized hydrolysis conditions, hemicellulose conversion (95.03%) with the highest XOS yield of 351.46 mg/g was achieved at 40 °C (pH 4.0) after 48 h incubation with Xyn2:AnabfA ratio of 400 U:10 U. Xylobiose (135.42 mg/g) is the main XOS product with xylotriose (102.04 mg/g) being the second highest. The high production of XOS yield from the pretreated kenaf stem demonstrated the biotechnological potential of the kenaf in the future. Elsevier 2016 Article PeerReviewed text en http://eprints.uthm.edu.my/5266/1/AJ%202016%20%2874%29.pdf Wan Azelee, Nur Izyan and Md Jahim, Jamaliah and Ismail, Ahmad Fauzi and Mohamad Fuzi, Siti Fatimah Zaharah and A. Rahman, Roshanida and Md Illias, Rosli (2016) High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis. Industrial Crops and Products, 81. pp. 11-19. ISSN 0926-6690 http://dx.doi.org/10.1016/j.indcrop.2015.11.038 |
| spellingShingle | QD Chemistry Wan Azelee, Nur Izyan Md Jahim, Jamaliah Ismail, Ahmad Fauzi Mohamad Fuzi, Siti Fatimah Zaharah A. Rahman, Roshanida Md Illias, Rosli High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis |
| title | High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis |
| title_full | High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis |
| title_fullStr | High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis |
| title_full_unstemmed | High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis |
| title_short | High xylooligosaccharides (XOS) production from pretreated kenaf stem by enzyme mixture hydrolysis |
| title_sort | high xylooligosaccharides (xos) production from pretreated kenaf stem by enzyme mixture hydrolysis |
| topic | QD Chemistry |
| url | http://eprints.uthm.edu.my/5266/ http://eprints.uthm.edu.my/5266/ http://eprints.uthm.edu.my/5266/1/AJ%202016%20%2874%29.pdf |