A study on antimycobacterial activity and phytochemical constituents of Dipterocarpus sublamellatus foxw. hexane extract (DSHE)
Purpose: To investigate the antimycobacterial activity and to profile the phytochemicals of Dipterocarpus sublamellatus hexane extract (DSHE). Methodology: Using Mycobacterium smegmatis as a rapid screening model of TB strain, the bacterial cells were treated with 4-fold of Minimum Inhibitory Concen...
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| Format: | Article |
| Language: | English |
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Advanced Scientific Research
2020
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| Online Access: | http://eprints.uthm.edu.my/5103/ http://eprints.uthm.edu.my/5103/1/AJ%202020%20%2894%29.pdf |
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| author | Sabran, Siti Fatimah Mazlun, Muhamad Harith Abadullah, Zunoliza Mohamed, Maryati Abu Bakar, Mohd. Fadzelly Pa’ee, Furzani Cuevas Linatoc, Alona |
| author_facet | Sabran, Siti Fatimah Mazlun, Muhamad Harith Abadullah, Zunoliza Mohamed, Maryati Abu Bakar, Mohd. Fadzelly Pa’ee, Furzani Cuevas Linatoc, Alona |
| author_sort | Sabran, Siti Fatimah |
| building | UTHM Institutional Repository |
| collection | Online Access |
| description | Purpose: To investigate the antimycobacterial activity and to profile the phytochemicals of Dipterocarpus sublamellatus hexane extract (DSHE). Methodology: Using Mycobacterium smegmatis as a rapid screening model of TB strain, the bacterial cells were treated with 4-fold of Minimum Inhibitory Concentration (MIC=0.78 mg/mL) of DSHE. Significant cellular leakage of DSHE-treated cells were quantified spectrophotometrically at 280 nm. Microscopic study using FE-SEM was employed to indicate any morphological changes induced on bacterial cell shape. Phytochemical profiles of DSHE was conducted using GC-MS analysis. Results: The bacterial cells treated with 4-fold of Minimum Inhibitory Concentration (MIC=0.78 mg/mL) of DSHE rapidly inhibited the bacterial cell growth as early as 8 h of treatment. cell membrane integrity were severely interrupted and losing the vital constituents for cell survival and growth. Substantial morphological changes were induced on bacterial cell shape, supporting the interaction of DSHE with cell wall/membrane of M. smegmatis. GC-MS analysis of DSHE suggested that pyrazole and phenanthroline derivatives were among 23 major phytochemical constituents that potentially contributed to the antimycobacterial activity. Applications/Originality/Value: A further study in this direction potentially leads to the discovery of target-specific antimycobacterial agents and provides a basis that this timber species has to be conserved and managed sustainably. |
| first_indexed | 2025-11-15T20:10:42Z |
| format | Article |
| id | uthm-5103 |
| institution | Universiti Tun Hussein Onn Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:10:42Z |
| publishDate | 2020 |
| publisher | Advanced Scientific Research |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uthm-51032022-01-05T08:31:29Z http://eprints.uthm.edu.my/5103/ A study on antimycobacterial activity and phytochemical constituents of Dipterocarpus sublamellatus foxw. hexane extract (DSHE) Sabran, Siti Fatimah Mazlun, Muhamad Harith Abadullah, Zunoliza Mohamed, Maryati Abu Bakar, Mohd. Fadzelly Pa’ee, Furzani Cuevas Linatoc, Alona T Technology (General) QP501-801 Animal biochemistry Purpose: To investigate the antimycobacterial activity and to profile the phytochemicals of Dipterocarpus sublamellatus hexane extract (DSHE). Methodology: Using Mycobacterium smegmatis as a rapid screening model of TB strain, the bacterial cells were treated with 4-fold of Minimum Inhibitory Concentration (MIC=0.78 mg/mL) of DSHE. Significant cellular leakage of DSHE-treated cells were quantified spectrophotometrically at 280 nm. Microscopic study using FE-SEM was employed to indicate any morphological changes induced on bacterial cell shape. Phytochemical profiles of DSHE was conducted using GC-MS analysis. Results: The bacterial cells treated with 4-fold of Minimum Inhibitory Concentration (MIC=0.78 mg/mL) of DSHE rapidly inhibited the bacterial cell growth as early as 8 h of treatment. cell membrane integrity were severely interrupted and losing the vital constituents for cell survival and growth. Substantial morphological changes were induced on bacterial cell shape, supporting the interaction of DSHE with cell wall/membrane of M. smegmatis. GC-MS analysis of DSHE suggested that pyrazole and phenanthroline derivatives were among 23 major phytochemical constituents that potentially contributed to the antimycobacterial activity. Applications/Originality/Value: A further study in this direction potentially leads to the discovery of target-specific antimycobacterial agents and provides a basis that this timber species has to be conserved and managed sustainably. Advanced Scientific Research 2020 Article PeerReviewed text en http://eprints.uthm.edu.my/5103/1/AJ%202020%20%2894%29.pdf Sabran, Siti Fatimah and Mazlun, Muhamad Harith and Abadullah, Zunoliza and Mohamed, Maryati and Abu Bakar, Mohd. Fadzelly and Pa’ee, Furzani and Cuevas Linatoc, Alona (2020) A study on antimycobacterial activity and phytochemical constituents of Dipterocarpus sublamellatus foxw. hexane extract (DSHE). International Journal of Pharmaceutical Research, 12 (1). pp. 651-659. ISSN 0975-2366 https://dx.doi.org/ 10.31838/ijpr/2020.12.01.133 |
| spellingShingle | T Technology (General) QP501-801 Animal biochemistry Sabran, Siti Fatimah Mazlun, Muhamad Harith Abadullah, Zunoliza Mohamed, Maryati Abu Bakar, Mohd. Fadzelly Pa’ee, Furzani Cuevas Linatoc, Alona A study on antimycobacterial activity and phytochemical constituents of Dipterocarpus sublamellatus foxw. hexane extract (DSHE) |
| title | A study on antimycobacterial activity and phytochemical constituents of Dipterocarpus sublamellatus foxw. hexane extract (DSHE) |
| title_full | A study on antimycobacterial activity and phytochemical constituents of Dipterocarpus sublamellatus foxw. hexane extract (DSHE) |
| title_fullStr | A study on antimycobacterial activity and phytochemical constituents of Dipterocarpus sublamellatus foxw. hexane extract (DSHE) |
| title_full_unstemmed | A study on antimycobacterial activity and phytochemical constituents of Dipterocarpus sublamellatus foxw. hexane extract (DSHE) |
| title_short | A study on antimycobacterial activity and phytochemical constituents of Dipterocarpus sublamellatus foxw. hexane extract (DSHE) |
| title_sort | study on antimycobacterial activity and phytochemical constituents of dipterocarpus sublamellatus foxw. hexane extract (dshe) |
| topic | T Technology (General) QP501-801 Animal biochemistry |
| url | http://eprints.uthm.edu.my/5103/ http://eprints.uthm.edu.my/5103/ http://eprints.uthm.edu.my/5103/1/AJ%202020%20%2894%29.pdf |