Oxidative enzymes from newly local strain Aspergillus iizukae EAN605 using pumpkin peels as a production substrate: optimized production, characterization, application and techno-economic analysis
The present study deals with optimizing, producing, characterizing, application and techno- economic analysis of oxidative enzymes [Laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP)] from Aspergillus iizukae EAN605 in submerged fermentation process using pumpkin peels as a produ...
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| Format: | Article |
| Language: | English |
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Elsevier
2020
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| Online Access: | http://eprints.uthm.edu.my/4988/ http://eprints.uthm.edu.my/4988/1/AJ%202020%20%2864%29.pdf |
| _version_ | 1848888433650434048 |
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| author | Noman, Efaq Al-Gheethi, A. A. A. Talip, Balkis Mohamed, Radin Kassim, Amir Hashim |
| author_facet | Noman, Efaq Al-Gheethi, A. A. A. Talip, Balkis Mohamed, Radin Kassim, Amir Hashim |
| author_sort | Noman, Efaq |
| building | UTHM Institutional Repository |
| collection | Online Access |
| description | The present study deals with optimizing, producing, characterizing, application and techno- economic analysis of oxidative enzymes [Laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP)] from Aspergillus iizukae EAN605 in submerged fermentation process using pumpkin peels as a production substrate. The best operating parameters for producing Lac, MnP and LiP (6.15, 2.58 and 127.99 U mg−1 respectively) were recorded with 20 g 100 mL−1 of substrate, 4.6 mL 100 mL−1 of inoculum size at pH 5.5 after 10 days. The crude enzyme exhibited high stability at pH (3–9) and temperatures (20−60 °C). Km (Michaelis–Menten) of Lac, MnP and LiP crude enzyme was 2.25, 1.79 and 0.72mM respectively. The decolourization of Remazol Brilliant Blue R by the crude enzyme was 84.84 %. The techno-economic analysis was assessed for a production unit with an annual operating time for enzymatic production and application is 7920 h/year and 100 m3 of the capacity. The process would produce 27,000 cm3 of crude enzyme with a price of USD 0.107 per cm3 compared to USD 1 per cm3 of the current commercial enzyme. The findings indicated that pumpkin peels have potential as a production substrate for oxidative enzymes from A. iizukae EAN605 and is economically feasible. |
| first_indexed | 2025-11-15T20:10:13Z |
| format | Article |
| id | uthm-4988 |
| institution | Universiti Tun Hussein Onn Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:10:13Z |
| publishDate | 2020 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uthm-49882022-01-03T02:56:30Z http://eprints.uthm.edu.my/4988/ Oxidative enzymes from newly local strain Aspergillus iizukae EAN605 using pumpkin peels as a production substrate: optimized production, characterization, application and techno-economic analysis Noman, Efaq Al-Gheethi, A. A. A. Talip, Balkis Mohamed, Radin Kassim, Amir Hashim T Technology (General) TP200-248 Chemicals: Manufacture, use, etc. The present study deals with optimizing, producing, characterizing, application and techno- economic analysis of oxidative enzymes [Laccase (Lac), manganese peroxidase (MnP), and lignin peroxidase (LiP)] from Aspergillus iizukae EAN605 in submerged fermentation process using pumpkin peels as a production substrate. The best operating parameters for producing Lac, MnP and LiP (6.15, 2.58 and 127.99 U mg−1 respectively) were recorded with 20 g 100 mL−1 of substrate, 4.6 mL 100 mL−1 of inoculum size at pH 5.5 after 10 days. The crude enzyme exhibited high stability at pH (3–9) and temperatures (20−60 °C). Km (Michaelis–Menten) of Lac, MnP and LiP crude enzyme was 2.25, 1.79 and 0.72mM respectively. The decolourization of Remazol Brilliant Blue R by the crude enzyme was 84.84 %. The techno-economic analysis was assessed for a production unit with an annual operating time for enzymatic production and application is 7920 h/year and 100 m3 of the capacity. The process would produce 27,000 cm3 of crude enzyme with a price of USD 0.107 per cm3 compared to USD 1 per cm3 of the current commercial enzyme. The findings indicated that pumpkin peels have potential as a production substrate for oxidative enzymes from A. iizukae EAN605 and is economically feasible. Elsevier 2020 Article PeerReviewed text en http://eprints.uthm.edu.my/4988/1/AJ%202020%20%2864%29.pdf Noman, Efaq and Al-Gheethi, A. A. and A. Talip, Balkis and Mohamed, Radin and Kassim, Amir Hashim (2020) Oxidative enzymes from newly local strain Aspergillus iizukae EAN605 using pumpkin peels as a production substrate: optimized production, characterization, application and techno-economic analysis. Journal of Hazardous Materials, 386. pp. 1-13. ISSN 0304-3894 https://doi.org/10.1016/j.jhazmat.2019.121954 |
| spellingShingle | T Technology (General) TP200-248 Chemicals: Manufacture, use, etc. Noman, Efaq Al-Gheethi, A. A. A. Talip, Balkis Mohamed, Radin Kassim, Amir Hashim Oxidative enzymes from newly local strain Aspergillus iizukae EAN605 using pumpkin peels as a production substrate: optimized production, characterization, application and techno-economic analysis |
| title | Oxidative enzymes from newly local strain Aspergillus iizukae EAN605 using pumpkin peels as a production substrate: optimized production, characterization, application and techno-economic analysis |
| title_full | Oxidative enzymes from newly local strain Aspergillus iizukae EAN605 using pumpkin peels as a production substrate: optimized production, characterization, application and techno-economic analysis |
| title_fullStr | Oxidative enzymes from newly local strain Aspergillus iizukae EAN605 using pumpkin peels as a production substrate: optimized production, characterization, application and techno-economic analysis |
| title_full_unstemmed | Oxidative enzymes from newly local strain Aspergillus iizukae EAN605 using pumpkin peels as a production substrate: optimized production, characterization, application and techno-economic analysis |
| title_short | Oxidative enzymes from newly local strain Aspergillus iizukae EAN605 using pumpkin peels as a production substrate: optimized production, characterization, application and techno-economic analysis |
| title_sort | oxidative enzymes from newly local strain aspergillus iizukae ean605 using pumpkin peels as a production substrate: optimized production, characterization, application and techno-economic analysis |
| topic | T Technology (General) TP200-248 Chemicals: Manufacture, use, etc. |
| url | http://eprints.uthm.edu.my/4988/ http://eprints.uthm.edu.my/4988/ http://eprints.uthm.edu.my/4988/1/AJ%202020%20%2864%29.pdf |