Phytochemical constituents, antioxidant and antiproliferative properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia
The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properti...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2015
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| Online Access: | http://eprints.uthm.edu.my/4831/ http://eprints.uthm.edu.my/4831/1/AJ%202015%20%2835%29.pdf |
| _version_ | 1848888393574907904 |
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| author | Abu Bakar, Mohd Fadzelly Abdul Karim, Fifilyana Suleiman, Monica Isha, Azizul |
| author_facet | Abu Bakar, Mohd Fadzelly Abdul Karim, Fifilyana Suleiman, Monica Isha, Azizul |
| author_sort | Abu Bakar, Mohd Fadzelly |
| building | UTHM Institutional Repository |
| collection | Online Access |
| description | The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properties were evaluated by using FRAP, ABTS, and DPPH assays while the effects of L. borneensis on the proliferation of MCF-7 cell line were evaluated by using MTT assay. The results showed that the total phenolic and flavonoid contents were 12.42 ± 0.47 mg GAE/g and 9.36 ± 1.29 mg CE/g, respectively. The GC-MS analysis revealed the presence of at least 35 compounds. The extract was found to induce cytotoxicity against MCF-7 cell line with IC50 value of 47.33 ± 7.37 µg/mL. Cell cycle analysis showed that the extract induced significant arrest at G0/G1 at 24 hours of treatment. After 72 hours of treatment, the proportion of cells in G0/G1 and G2-M phases had decreased significantly as compared to their control. Apoptosis occurred during the first 24 hours and significantly increased to 30.8% after 72 hours of treatment. No activation of caspase 3 was observed. These findings suggest that L. borneensis extract has the potential as natural antioxidant and anticancer agents. |
| first_indexed | 2025-11-15T20:09:34Z |
| format | Article |
| id | uthm-4831 |
| institution | Universiti Tun Hussein Onn Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:09:34Z |
| publishDate | 2015 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uthm-48312021-12-21T07:11:18Z http://eprints.uthm.edu.my/4831/ Phytochemical constituents, antioxidant and antiproliferative properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia Abu Bakar, Mohd Fadzelly Abdul Karim, Fifilyana Suleiman, Monica Isha, Azizul QK Botany T Technology (General) The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properties were evaluated by using FRAP, ABTS, and DPPH assays while the effects of L. borneensis on the proliferation of MCF-7 cell line were evaluated by using MTT assay. The results showed that the total phenolic and flavonoid contents were 12.42 ± 0.47 mg GAE/g and 9.36 ± 1.29 mg CE/g, respectively. The GC-MS analysis revealed the presence of at least 35 compounds. The extract was found to induce cytotoxicity against MCF-7 cell line with IC50 value of 47.33 ± 7.37 µg/mL. Cell cycle analysis showed that the extract induced significant arrest at G0/G1 at 24 hours of treatment. After 72 hours of treatment, the proportion of cells in G0/G1 and G2-M phases had decreased significantly as compared to their control. Apoptosis occurred during the first 24 hours and significantly increased to 30.8% after 72 hours of treatment. No activation of caspase 3 was observed. These findings suggest that L. borneensis extract has the potential as natural antioxidant and anticancer agents. Hindawi Publishing Corporation 2015 Article PeerReviewed text en http://eprints.uthm.edu.my/4831/1/AJ%202015%20%2835%29.pdf Abu Bakar, Mohd Fadzelly and Abdul Karim, Fifilyana and Suleiman, Monica and Isha, Azizul (2015) Phytochemical constituents, antioxidant and antiproliferative properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia. Evidence-Based Complementary and Alternative Medicine. pp. 1-9. ISSN 1741-427X http://dx.doi.org/10.1155/2015/936215 |
| spellingShingle | QK Botany T Technology (General) Abu Bakar, Mohd Fadzelly Abdul Karim, Fifilyana Suleiman, Monica Isha, Azizul Phytochemical constituents, antioxidant and antiproliferative properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia |
| title | Phytochemical constituents, antioxidant and antiproliferative properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia |
| title_full | Phytochemical constituents, antioxidant and antiproliferative properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia |
| title_fullStr | Phytochemical constituents, antioxidant and antiproliferative properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia |
| title_full_unstemmed | Phytochemical constituents, antioxidant and antiproliferative properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia |
| title_short | Phytochemical constituents, antioxidant and antiproliferative properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia |
| title_sort | phytochemical constituents, antioxidant and antiproliferative properties of a liverwort, lepidozia borneensis stephani from mount kinabalu, sabah, malaysia |
| topic | QK Botany T Technology (General) |
| url | http://eprints.uthm.edu.my/4831/ http://eprints.uthm.edu.my/4831/ http://eprints.uthm.edu.my/4831/1/AJ%202015%20%2835%29.pdf |