Single cell analysis of neutrophils NETs by microscopic LSPR imaging system
A simple microengraving cell monitoring method for neutrophil extracellular traps (NETs) released from single neutrophils has been realized using a polydimethylsiloxane (PDMS) microwell array (MWA) sheet on a plasmon chip platform. An imbalance between NETs formation and the succeeding degradation (...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2020
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| Online Access: | http://eprints.uthm.edu.my/4717/ http://eprints.uthm.edu.my/4717/1/AJ%202020%20%289%29.pdf |
| _version_ | 1848888362450026496 |
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| author | Mohamed Ali, Riyaz Ahmad Mita, Daiki Espulgar, Wilfred Saito, Masato Nishide, Masayuki Takamatsu, Hyota Yoshikawa, Hiroyuki Tamiya, Eiichi |
| author_facet | Mohamed Ali, Riyaz Ahmad Mita, Daiki Espulgar, Wilfred Saito, Masato Nishide, Masayuki Takamatsu, Hyota Yoshikawa, Hiroyuki Tamiya, Eiichi |
| author_sort | Mohamed Ali, Riyaz Ahmad |
| building | UTHM Institutional Repository |
| collection | Online Access |
| description | A simple microengraving cell monitoring method for neutrophil extracellular traps (NETs) released from single neutrophils has been realized using a polydimethylsiloxane (PDMS) microwell array (MWA) sheet on a plasmon chip platform. An imbalance between NETs formation and the succeeding degradation (NETosis) are considered associated with autoimmune disease and its pathogenesis. Thus, an alternative platform that can conduct monitoring of this activity on single cell level at minimum cost but with great sensitivity is greatly desired. The developed MWA plasmon chips allow single cell isolation of neutrophils from 150 μL suspension (6.0 × 105 cells/mL) with an efficiency of 36.3%; 105 microwells with single cell condition. To demonstrate the utility of the chip, trapped cells were incubated between 2 to 4 h after introducing with 100 nM phorbol 12- myristate 13-acetate (PMA) before measurement. Under observation using a hyperspectral imaging system that allows high-throughput screening, the neutrophils stimulated by PMA solution show a significant release of fibrils and NETs after 4 h, with observed maximum areas between 314–758 μm2. An average absorption peak wavelength shows a redshift of Δλ = 1.5 nm as neutrophils release NETs. |
| first_indexed | 2025-11-15T20:09:05Z |
| format | Article |
| id | uthm-4717 |
| institution | Universiti Tun Hussein Onn Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:09:05Z |
| publishDate | 2020 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uthm-47172021-12-14T08:39:23Z http://eprints.uthm.edu.my/4717/ Single cell analysis of neutrophils NETs by microscopic LSPR imaging system Mohamed Ali, Riyaz Ahmad Mita, Daiki Espulgar, Wilfred Saito, Masato Nishide, Masayuki Takamatsu, Hyota Yoshikawa, Hiroyuki Tamiya, Eiichi QD Chemistry T Technology (General) A simple microengraving cell monitoring method for neutrophil extracellular traps (NETs) released from single neutrophils has been realized using a polydimethylsiloxane (PDMS) microwell array (MWA) sheet on a plasmon chip platform. An imbalance between NETs formation and the succeeding degradation (NETosis) are considered associated with autoimmune disease and its pathogenesis. Thus, an alternative platform that can conduct monitoring of this activity on single cell level at minimum cost but with great sensitivity is greatly desired. The developed MWA plasmon chips allow single cell isolation of neutrophils from 150 μL suspension (6.0 × 105 cells/mL) with an efficiency of 36.3%; 105 microwells with single cell condition. To demonstrate the utility of the chip, trapped cells were incubated between 2 to 4 h after introducing with 100 nM phorbol 12- myristate 13-acetate (PMA) before measurement. Under observation using a hyperspectral imaging system that allows high-throughput screening, the neutrophils stimulated by PMA solution show a significant release of fibrils and NETs after 4 h, with observed maximum areas between 314–758 μm2. An average absorption peak wavelength shows a redshift of Δλ = 1.5 nm as neutrophils release NETs. MDPI 2020 Article PeerReviewed text en http://eprints.uthm.edu.my/4717/1/AJ%202020%20%289%29.pdf Mohamed Ali, Riyaz Ahmad and Mita, Daiki and Espulgar, Wilfred and Saito, Masato and Nishide, Masayuki and Takamatsu, Hyota and Yoshikawa, Hiroyuki and Tamiya, Eiichi (2020) Single cell analysis of neutrophils NETs by microscopic LSPR imaging system. Micromachines, 11 (1). pp. 1-15. ISSN 2072-666X https://dx.doi.org/ 10.3390/mi11010052 |
| spellingShingle | QD Chemistry T Technology (General) Mohamed Ali, Riyaz Ahmad Mita, Daiki Espulgar, Wilfred Saito, Masato Nishide, Masayuki Takamatsu, Hyota Yoshikawa, Hiroyuki Tamiya, Eiichi Single cell analysis of neutrophils NETs by microscopic LSPR imaging system |
| title | Single cell analysis of neutrophils NETs by microscopic LSPR imaging system |
| title_full | Single cell analysis of neutrophils NETs by microscopic LSPR imaging system |
| title_fullStr | Single cell analysis of neutrophils NETs by microscopic LSPR imaging system |
| title_full_unstemmed | Single cell analysis of neutrophils NETs by microscopic LSPR imaging system |
| title_short | Single cell analysis of neutrophils NETs by microscopic LSPR imaging system |
| title_sort | single cell analysis of neutrophils nets by microscopic lspr imaging system |
| topic | QD Chemistry T Technology (General) |
| url | http://eprints.uthm.edu.my/4717/ http://eprints.uthm.edu.my/4717/ http://eprints.uthm.edu.my/4717/1/AJ%202020%20%289%29.pdf |