Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase: effects of reaction conditions for optimum hemicellulose hydrolysis
An efficient hemicellulose hydrolysis has now become a crucial step for xylooligosaccharides production for food additives and nutraceuticals industries. In this study, xylanase hydrolysis of pretreated hemicellulosic kenaf (Hibiscus cannabinus) for substantial hemicellulose hydrolysis was demonstra...
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| Format: | Article |
| Language: | English |
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Science Publications
2016
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| Online Access: | http://eprints.uthm.edu.my/4196/ http://eprints.uthm.edu.my/4196/1/AJ%202016%20%2824%29.pdf |
| _version_ | 1848888221741613056 |
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| author | Mohamad Fuzi, Siti Fatimah Zaharah Wan Azelee, Nur Izyan Md Jahim, Jamaliah Ismail, Ahmad Fauzi A. Rahman, Roshanida Ghazali, Nazlee Faisal Md Illias, Rosli |
| author_facet | Mohamad Fuzi, Siti Fatimah Zaharah Wan Azelee, Nur Izyan Md Jahim, Jamaliah Ismail, Ahmad Fauzi A. Rahman, Roshanida Ghazali, Nazlee Faisal Md Illias, Rosli |
| author_sort | Mohamad Fuzi, Siti Fatimah Zaharah |
| building | UTHM Institutional Repository |
| collection | Online Access |
| description | An efficient hemicellulose hydrolysis has now become a crucial step for xylooligosaccharides production for food additives and nutraceuticals industries. In this study, xylanase hydrolysis of pretreated hemicellulosic kenaf (Hibiscus cannabinus) for substantial hemicellulose hydrolysis was demonstrated. Experiments were performed primarily to investigate the effect of several critical reaction conditions towards the enzymatic hydrolysis of the pre-treated kenaf. The effects of various ranges of reaction conditions (substrate loading, xylanase loading, pH, temperature and time) were systematically and thoroughly studied. The study of hemicellulosic kenaf conversion using single enzyme was proven to effectively yield up to 59% of conversion after 48 h incubation using 3% (w/v) of substrate loading and 400 U of xylanase loading at 50°C in pH 4.0 buffer systems. The use of a single xylanase on the pre-treated kenaf was a key parameter in understanding the action of the main hemicellulose degrading enzyme towards hydrolysing the hemicellulose backbone of the pre-treated kenaf. Enzyme feeding strategy was also conducted and the results revealed that a batch feeding strategy of 400 U xylanase loading yielded the highest hemicellulose hydrolysis compared to the other fed batch enzyme feeding strategy. It was concluded that each reaction conditions and enzyme feeding strategy gave a significant impact towards the enzymatic hydrolysis and the use of optimum reaction conditions resulted in a high product yield at the end of the reaction. With the discovery of the importance of each reaction conditions involved in a hydrolysis, this study highlights the need for a comprehensive investigation on the reaction conditions using a mixture of enzymes towards achieving a complete hydrolysis with a higher product yield. |
| first_indexed | 2025-11-15T20:06:50Z |
| format | Article |
| id | uthm-4196 |
| institution | Universiti Tun Hussein Onn Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T20:06:50Z |
| publishDate | 2016 |
| publisher | Science Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uthm-41962021-11-28T07:43:27Z http://eprints.uthm.edu.my/4196/ Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase: effects of reaction conditions for optimum hemicellulose hydrolysis Mohamad Fuzi, Siti Fatimah Zaharah Wan Azelee, Nur Izyan Md Jahim, Jamaliah Ismail, Ahmad Fauzi A. Rahman, Roshanida Ghazali, Nazlee Faisal Md Illias, Rosli QA299.6-433 Analysis An efficient hemicellulose hydrolysis has now become a crucial step for xylooligosaccharides production for food additives and nutraceuticals industries. In this study, xylanase hydrolysis of pretreated hemicellulosic kenaf (Hibiscus cannabinus) for substantial hemicellulose hydrolysis was demonstrated. Experiments were performed primarily to investigate the effect of several critical reaction conditions towards the enzymatic hydrolysis of the pre-treated kenaf. The effects of various ranges of reaction conditions (substrate loading, xylanase loading, pH, temperature and time) were systematically and thoroughly studied. The study of hemicellulosic kenaf conversion using single enzyme was proven to effectively yield up to 59% of conversion after 48 h incubation using 3% (w/v) of substrate loading and 400 U of xylanase loading at 50°C in pH 4.0 buffer systems. The use of a single xylanase on the pre-treated kenaf was a key parameter in understanding the action of the main hemicellulose degrading enzyme towards hydrolysing the hemicellulose backbone of the pre-treated kenaf. Enzyme feeding strategy was also conducted and the results revealed that a batch feeding strategy of 400 U xylanase loading yielded the highest hemicellulose hydrolysis compared to the other fed batch enzyme feeding strategy. It was concluded that each reaction conditions and enzyme feeding strategy gave a significant impact towards the enzymatic hydrolysis and the use of optimum reaction conditions resulted in a high product yield at the end of the reaction. With the discovery of the importance of each reaction conditions involved in a hydrolysis, this study highlights the need for a comprehensive investigation on the reaction conditions using a mixture of enzymes towards achieving a complete hydrolysis with a higher product yield. Science Publications 2016 Article PeerReviewed text en http://eprints.uthm.edu.my/4196/1/AJ%202016%20%2824%29.pdf Mohamad Fuzi, Siti Fatimah Zaharah and Wan Azelee, Nur Izyan and Md Jahim, Jamaliah and Ismail, Ahmad Fauzi and A. Rahman, Roshanida and Ghazali, Nazlee Faisal and Md Illias, Rosli (2016) Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase: effects of reaction conditions for optimum hemicellulose hydrolysis. American Journal of Agricultural and Biological Sciences, 11 (2). p. 54. ISSN 1557-4989 https://doi.org/10.3844/ajabssp.2016.54.66 |
| spellingShingle | QA299.6-433 Analysis Mohamad Fuzi, Siti Fatimah Zaharah Wan Azelee, Nur Izyan Md Jahim, Jamaliah Ismail, Ahmad Fauzi A. Rahman, Roshanida Ghazali, Nazlee Faisal Md Illias, Rosli Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase: effects of reaction conditions for optimum hemicellulose hydrolysis |
| title | Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase: effects of reaction conditions for optimum hemicellulose hydrolysis |
| title_full | Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase: effects of reaction conditions for optimum hemicellulose hydrolysis |
| title_fullStr | Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase: effects of reaction conditions for optimum hemicellulose hydrolysis |
| title_full_unstemmed | Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase: effects of reaction conditions for optimum hemicellulose hydrolysis |
| title_short | Enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase: effects of reaction conditions for optimum hemicellulose hydrolysis |
| title_sort | enzymatic hydrolysis of pretreated kenaf using a recombinant xylanase: effects of reaction conditions for optimum hemicellulose hydrolysis |
| topic | QA299.6-433 Analysis |
| url | http://eprints.uthm.edu.my/4196/ http://eprints.uthm.edu.my/4196/ http://eprints.uthm.edu.my/4196/1/AJ%202016%20%2824%29.pdf |