Physical and biological characteristics of silver/ polyvinylpyrrolidone nanocomposite using pandanus atrocarpus
In the present study, synthesis and characterization of silver nanoparticle (AgNPs) and Polyvinylpyrrolidone (PVP) nanocomposite (PVP-AgNCs) and their biological applications were studied. AgNPs and PVP-AgNCs were synthesised by biological method using Pandanus atrocarpus extract (PAE) as a re...
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| Format: | Thesis |
| Language: | English English English |
| Published: |
2021
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/3927/ http://eprints.uthm.edu.my/3927/1/24p%20ABDULLAH%20HASAN%20JABBAR.pdf http://eprints.uthm.edu.my/3927/2/ABDULLAH%20HASAN%20JABBAR%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/3927/3/ABDULLAH%20HASAN%20JABBAR%20WATERMARK.pdf |
| Summary: | In the present study, synthesis and characterization of silver nanoparticle (AgNPs)
and Polyvinylpyrrolidone (PVP) nanocomposite (PVP-AgNCs) and their biological
applications were studied. AgNPs and PVP-AgNCs were synthesised by biological
method using Pandanus atrocarpus extract (PAE) as a reducing agent. The detailed
characterization of PAE-AgNPs and PVP-AgNPs was carried out using Ultraviolet�Visible (UV-Vis), Fourier Transform Infrared (FTIR), Field Emission Scanning
Electron Microscope (FESEM) and X-Ray Diffraction (XRD). UV-Vis peak of
PAE-AgNPs and PVP-AgNCs obtained at 432 nm and 540 nm respectively. FTIR's
prominent peaks in PAE and AgNPs and PVP-AgNCs do not considerably differ
from each other and are located at 3338, 2131, 1638, 1295, and 428. The prominent
peak at 3338 belongs to the –OH group. FESEM confirmed the production of
smallest PAE-AgNPs is of 14 nm in a spherical shape‘s range while smallest PVP�AgNCs was 44 nm. The Bragg‘s reflection values of 38°, 44°, 64°, and 77° were
obtained for PAE-AgNPs and PVP-AgNCs corresponding to the set of lattice planes
(111), (200), (220) and (311) at 2θ. This indicates the formation of face centred
cubic (fcc) crystalline structure of the nanostructure. The main findings include the
biological activities of the PAE, PAE-AgNPs PVP-AgNCs as cytotoxicity,
antioxidant, anti-enzymatic, and antiurolithitaic activities. Five different
concentration of sample tested (100 µg/mL - 500 µg/mL), all the results were dose
dependant. For cytotoxicity, as the concentration increases to 500 µg/mL, the
mortality was about 16.7 % for PAE-AgNPs, while 23.3 % for PVP-AgNCs. The
ABTS radical scavenging activity (% I) of PAE was recorded at 58.20 %, PAE�AgNPs at 64.20 %, and PVP/AgNC at 76.00 % at concentration of 500 µg/mL. The
absorbance values of PAE, PAE-AgNPs, and PVP-AgNC at 16 min in
antiurolithiatic assay was recorded at 0.299, 0.291 and 0.299, respectively, which are
significantly different (p < 0.05). Percent inhibition of anti-enzymatic activity of
PAE, PAE-AgNPs and PVP-AgNC was 42.30%, 48.70%, and 54.40%, respectively.
From our findings it is concluded that AgNPs, and PVP-AgNCs from Pandanus
atrocarpus is safe and eco-friendly, and hence needs to be studied in vivo |
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