The use of palm oil-basedwaste cooking oil to enhance the production of polyhydroxybutyrate [P(3HB)] by cupriavidus necator H16 strain

Waste cooking oil (WCO) is of increasing interest as an inexpensive feedstock to produce biodegradable plastic, poly(3- hydroxybutyrate) [P(3HB)]. In the present study, palm oil-basedWCO(PO-WCO) was obtained from nine different locations. Palm oil-based fresh cooking oil (PO-FCO) and PO-WCO were cha...

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Main Authors: Kamilah, Hanisah, Al-Gheethi, Adel, Yang, Tajul Aris, Sudesh, Kumar
Format: Article
Language:English
Published: Springer 2018
Subjects:
Online Access:http://eprints.uthm.edu.my/3125/
http://eprints.uthm.edu.my/3125/1/AJ%202019%20%2874%29.pdf
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author Kamilah, Hanisah
Al-Gheethi, Adel
Yang, Tajul Aris
Sudesh, Kumar
author_facet Kamilah, Hanisah
Al-Gheethi, Adel
Yang, Tajul Aris
Sudesh, Kumar
author_sort Kamilah, Hanisah
building UTHM Institutional Repository
collection Online Access
description Waste cooking oil (WCO) is of increasing interest as an inexpensive feedstock to produce biodegradable plastic, poly(3- hydroxybutyrate) [P(3HB)]. In the present study, palm oil-basedWCO(PO-WCO) was obtained from nine different locations. Palm oil-based fresh cooking oil (PO-FCO) and PO-WCO were characterised via proximate and physicochemical analysis, prior to being used as carbon sources for the biosynthesis of P(3HB) using Cupriavidus necator H16. It was shown that the free fatty acid, peroxide value, and saturated compounds in all batches of PO-WCO were higher compared to those in the PO-FCO. The cells produced 60–80 wt% P(3HB) with dry cell weight of 14–17 g/L. The weight average molecular weight (Mw) was found to be 1.8 × 106 Da with a polydispersity (Mw/Mn) of 2.7 when PO-WCO was used as the carbon source. The PO-WCO was found to be suitable to be used as a sustainable carbon source for cell growth and P(3HB) biosynthesis.
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spelling uthm-31252021-11-16T06:57:11Z http://eprints.uthm.edu.my/3125/ The use of palm oil-basedwaste cooking oil to enhance the production of polyhydroxybutyrate [P(3HB)] by cupriavidus necator H16 strain Kamilah, Hanisah Al-Gheethi, Adel Yang, Tajul Aris Sudesh, Kumar TD429.5-480.7 Water purification. Water treatment and conditioning. Saline water conversion Waste cooking oil (WCO) is of increasing interest as an inexpensive feedstock to produce biodegradable plastic, poly(3- hydroxybutyrate) [P(3HB)]. In the present study, palm oil-basedWCO(PO-WCO) was obtained from nine different locations. Palm oil-based fresh cooking oil (PO-FCO) and PO-WCO were characterised via proximate and physicochemical analysis, prior to being used as carbon sources for the biosynthesis of P(3HB) using Cupriavidus necator H16. It was shown that the free fatty acid, peroxide value, and saturated compounds in all batches of PO-WCO were higher compared to those in the PO-FCO. The cells produced 60–80 wt% P(3HB) with dry cell weight of 14–17 g/L. The weight average molecular weight (Mw) was found to be 1.8 × 106 Da with a polydispersity (Mw/Mn) of 2.7 when PO-WCO was used as the carbon source. The PO-WCO was found to be suitable to be used as a sustainable carbon source for cell growth and P(3HB) biosynthesis. Springer 2018 Article PeerReviewed text en http://eprints.uthm.edu.my/3125/1/AJ%202019%20%2874%29.pdf Kamilah, Hanisah and Al-Gheethi, Adel and Yang, Tajul Aris and Sudesh, Kumar (2018) The use of palm oil-basedwaste cooking oil to enhance the production of polyhydroxybutyrate [P(3HB)] by cupriavidus necator H16 strain. Arabian Journal for Science and Engineering, 43. pp. 3453-3463. ISSN 2193-567X https://doi.org/10.1007/s13369-018-3118-1
spellingShingle TD429.5-480.7 Water purification. Water treatment and conditioning. Saline water conversion
Kamilah, Hanisah
Al-Gheethi, Adel
Yang, Tajul Aris
Sudesh, Kumar
The use of palm oil-basedwaste cooking oil to enhance the production of polyhydroxybutyrate [P(3HB)] by cupriavidus necator H16 strain
title The use of palm oil-basedwaste cooking oil to enhance the production of polyhydroxybutyrate [P(3HB)] by cupriavidus necator H16 strain
title_full The use of palm oil-basedwaste cooking oil to enhance the production of polyhydroxybutyrate [P(3HB)] by cupriavidus necator H16 strain
title_fullStr The use of palm oil-basedwaste cooking oil to enhance the production of polyhydroxybutyrate [P(3HB)] by cupriavidus necator H16 strain
title_full_unstemmed The use of palm oil-basedwaste cooking oil to enhance the production of polyhydroxybutyrate [P(3HB)] by cupriavidus necator H16 strain
title_short The use of palm oil-basedwaste cooking oil to enhance the production of polyhydroxybutyrate [P(3HB)] by cupriavidus necator H16 strain
title_sort use of palm oil-basedwaste cooking oil to enhance the production of polyhydroxybutyrate [p(3hb)] by cupriavidus necator h16 strain
topic TD429.5-480.7 Water purification. Water treatment and conditioning. Saline water conversion
url http://eprints.uthm.edu.my/3125/
http://eprints.uthm.edu.my/3125/
http://eprints.uthm.edu.my/3125/1/AJ%202019%20%2874%29.pdf