Production of lipopeptide biosurfactant by Kurthia gibsonii KH2 and their synergistic action in biodecolourisation of textile wastewater
Textile dyes are recalcitrant molecules and contain a high level of chemicals and colour which poses a serious challenge to surrounding environments. Therefore, this study aims to produce biosurfactant and investigate the synergistic action on decolourisation of textile dyes by the combination of...
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| Format: | Article |
| Language: | English |
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Elsevier
2021
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| Online Access: | http://eprints.uthm.edu.my/2321/ http://eprints.uthm.edu.my/2321/1/J12278_9b6371bb06af4cc41d144ce45d4202d6.pdf |
| _version_ | 1848887707702394880 |
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| author | Mohd Nor, Farhah Husna Abdullah, Shakila Yuniarto, Adhi Ibrahim, Zaharah Md Nor, Muhamad Hanif Tony Hadibarata, Tony Hadibarata |
| author_facet | Mohd Nor, Farhah Husna Abdullah, Shakila Yuniarto, Adhi Ibrahim, Zaharah Md Nor, Muhamad Hanif Tony Hadibarata, Tony Hadibarata |
| author_sort | Mohd Nor, Farhah Husna |
| building | UTHM Institutional Repository |
| collection | Online Access |
| description | Textile dyes are recalcitrant molecules and contain a high level of chemicals and colour
which poses a serious challenge to surrounding environments. Therefore, this study
aims to produce biosurfactant and investigate the synergistic action on decolourisation
of textile dyes by the combination of bacteria and biosurfactant. An effective dye
degrading strain and biosurfactant-producer, Kurthia gibsonii KH2, was isolated from
textile wastewater using molasses as the only source of carbon and energy. The isolates
were identified and screened for biosurfactant production using haemolytic activity,
oil spreading technique, drop collapse test and emulsification index. Fourier Transform
Infrared Spectrum (FTIR) and Thin Layer Chromatography (TLC) analyses were carried out
to detect the type of biosurfactant. The effect of different physicochemical parameters
on textile wastewater decolourisation was assessed within 24 h. The Kurthia gibsonii
KH2 showed positive results for haemolytic activity, oil spreading technique, and drop
collapse test. The emulsification test (E24) revealed that Kurthia gibsonii KH2 had a
higher emulsification index of 63%. FTIR and TLC analyses indicated that the biosurfactant
was a lipopeptide and was formed with a yield of 2 gL−1. The synergistic activity of
Kurthia gibsonii KH2 and lipopeptide biosurfactant resulted in decolourisation levels
of 85% at 100 mg/L concentration and pH 7 was recorded at 168 h of incubation.
The high attributes of these combinations and the phytotoxicity tests implied that the
metabolites were less toxic, making it a promising option for the biodecolourisation and
biodegradation of industrial textile wastewater and various environmental conditions. |
| first_indexed | 2025-11-15T19:58:40Z |
| format | Article |
| id | uthm-2321 |
| institution | Universiti Tun Hussein Onn Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:58:40Z |
| publishDate | 2021 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uthm-23212021-10-20T01:35:27Z http://eprints.uthm.edu.my/2321/ Production of lipopeptide biosurfactant by Kurthia gibsonii KH2 and their synergistic action in biodecolourisation of textile wastewater Mohd Nor, Farhah Husna Abdullah, Shakila Yuniarto, Adhi Ibrahim, Zaharah Md Nor, Muhamad Hanif Tony Hadibarata, Tony Hadibarata TD511-780 Sewage collection and disposal systems. Sewerage Textile dyes are recalcitrant molecules and contain a high level of chemicals and colour which poses a serious challenge to surrounding environments. Therefore, this study aims to produce biosurfactant and investigate the synergistic action on decolourisation of textile dyes by the combination of bacteria and biosurfactant. An effective dye degrading strain and biosurfactant-producer, Kurthia gibsonii KH2, was isolated from textile wastewater using molasses as the only source of carbon and energy. The isolates were identified and screened for biosurfactant production using haemolytic activity, oil spreading technique, drop collapse test and emulsification index. Fourier Transform Infrared Spectrum (FTIR) and Thin Layer Chromatography (TLC) analyses were carried out to detect the type of biosurfactant. The effect of different physicochemical parameters on textile wastewater decolourisation was assessed within 24 h. The Kurthia gibsonii KH2 showed positive results for haemolytic activity, oil spreading technique, and drop collapse test. The emulsification test (E24) revealed that Kurthia gibsonii KH2 had a higher emulsification index of 63%. FTIR and TLC analyses indicated that the biosurfactant was a lipopeptide and was formed with a yield of 2 gL−1. The synergistic activity of Kurthia gibsonii KH2 and lipopeptide biosurfactant resulted in decolourisation levels of 85% at 100 mg/L concentration and pH 7 was recorded at 168 h of incubation. The high attributes of these combinations and the phytotoxicity tests implied that the metabolites were less toxic, making it a promising option for the biodecolourisation and biodegradation of industrial textile wastewater and various environmental conditions. Elsevier 2021 Article PeerReviewed text en http://eprints.uthm.edu.my/2321/1/J12278_9b6371bb06af4cc41d144ce45d4202d6.pdf Mohd Nor, Farhah Husna and Abdullah, Shakila and Yuniarto, Adhi and Ibrahim, Zaharah and Md Nor, Muhamad Hanif and Tony Hadibarata, Tony Hadibarata (2021) Production of lipopeptide biosurfactant by Kurthia gibsonii KH2 and their synergistic action in biodecolourisation of textile wastewater. Environmental Technology & Innovation, 22. pp. 2-10. ISSN 2352-1864 https://doi.org/10.1016/j.eti.2021.101533 |
| spellingShingle | TD511-780 Sewage collection and disposal systems. Sewerage Mohd Nor, Farhah Husna Abdullah, Shakila Yuniarto, Adhi Ibrahim, Zaharah Md Nor, Muhamad Hanif Tony Hadibarata, Tony Hadibarata Production of lipopeptide biosurfactant by Kurthia gibsonii KH2 and their synergistic action in biodecolourisation of textile wastewater |
| title | Production of lipopeptide biosurfactant by Kurthia gibsonii
KH2 and their synergistic action in biodecolourisation of
textile wastewater |
| title_full | Production of lipopeptide biosurfactant by Kurthia gibsonii
KH2 and their synergistic action in biodecolourisation of
textile wastewater |
| title_fullStr | Production of lipopeptide biosurfactant by Kurthia gibsonii
KH2 and their synergistic action in biodecolourisation of
textile wastewater |
| title_full_unstemmed | Production of lipopeptide biosurfactant by Kurthia gibsonii
KH2 and their synergistic action in biodecolourisation of
textile wastewater |
| title_short | Production of lipopeptide biosurfactant by Kurthia gibsonii
KH2 and their synergistic action in biodecolourisation of
textile wastewater |
| title_sort | production of lipopeptide biosurfactant by kurthia gibsonii
kh2 and their synergistic action in biodecolourisation of
textile wastewater |
| topic | TD511-780 Sewage collection and disposal systems. Sewerage |
| url | http://eprints.uthm.edu.my/2321/ http://eprints.uthm.edu.my/2321/ http://eprints.uthm.edu.my/2321/1/J12278_9b6371bb06af4cc41d144ce45d4202d6.pdf |