Evaluation of Phyllanthus niruri L. from Malaysia for In-vitro Anti-Urolithiatic properties by different solvent extraction
The Phyllanthus niruri is traditionally used for curing of kidney disorders and urinary stones in Malaysia. Hence the current work was aimed to evaluate the effect of different solvents extract (n- hexane, ethyl acetate, methanol and water) of P. niruri for in vitro anti-urolithiatic properties in...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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2021
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/1877/ http://eprints.uthm.edu.my/1877/1/J12190_a9f73c6a96707e307554d0ecb28648d5.pdf |
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| author | Gul, Muhammad Tayyab Muhammad, Norhayati Pauzi, Aslia Natasha Abu Bakar, Mohd Fadzelly A. Talip, Balkis Abdullah, Norazlin Abdul Rahim, Nur Fazira Syukriah, Wan Nur Ain Marzuki, Wan B. Din, Laily Ibrahime, Nazlina |
| author_facet | Gul, Muhammad Tayyab Muhammad, Norhayati Pauzi, Aslia Natasha Abu Bakar, Mohd Fadzelly A. Talip, Balkis Abdullah, Norazlin Abdul Rahim, Nur Fazira Syukriah, Wan Nur Ain Marzuki, Wan B. Din, Laily Ibrahime, Nazlina |
| author_sort | Gul, Muhammad Tayyab |
| building | UTHM Institutional Repository |
| collection | Online Access |
| description | The Phyllanthus niruri is traditionally used for curing of kidney disorders and urinary stones
in Malaysia. Hence the current work was aimed to evaluate the effect of different solvents extract (n-
hexane, ethyl acetate, methanol and water) of P. niruri for in vitro anti-urolithiatic properties in terms of
inhibition activity on CaOx by using the rate of CaOx aggregation assay and dissolution of calcium oxalate
(CaOx) crystal by using titrimetry method. Cystone was used as positive control. The effects of cystone
on slope of nucleation and aggregation as well as growth of CaOx were evaluated spectrophotometrically.
The highest yield percentage of P.niruri was occupied by methanol (5.74 %). The maximum inhibition
against aggregation of CaOx crystals was also occupied by methanol (66.67 % ± 1.61) and was comprised
with alkaloid, steroid, terpenoid and tannin. Dissolution effect on calcium oxalate crystals indicates that
the aqueous extracts of P. niruri was found to be more effective in dissolution of CaOx with 63.33 % ±
1.44. P. niruri significantly (P < 0.05) inhibited the slope of nucleation and aggregation of CaOx crystallization,
and reduced the crystal density. The results of the present study confirmed that P. niruri leaves can be used
as remedial mediator for urolithiasis. However, further studies are required for isolation and identification
of active constituents and their in-vivo confirmation. |
| first_indexed | 2025-11-15T19:56:41Z |
| format | Article |
| id | uthm-1877 |
| institution | Universiti Tun Hussein Onn Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:56:41Z |
| publishDate | 2021 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | uthm-18772021-10-18T06:56:55Z http://eprints.uthm.edu.my/1877/ Evaluation of Phyllanthus niruri L. from Malaysia for In-vitro Anti-Urolithiatic properties by different solvent extraction Gul, Muhammad Tayyab Muhammad, Norhayati Pauzi, Aslia Natasha Abu Bakar, Mohd Fadzelly A. Talip, Balkis Abdullah, Norazlin Abdul Rahim, Nur Fazira Syukriah, Wan Nur Ain Marzuki, Wan B. Din, Laily Ibrahime, Nazlina TP155-156 Chemical engineering The Phyllanthus niruri is traditionally used for curing of kidney disorders and urinary stones in Malaysia. Hence the current work was aimed to evaluate the effect of different solvents extract (n- hexane, ethyl acetate, methanol and water) of P. niruri for in vitro anti-urolithiatic properties in terms of inhibition activity on CaOx by using the rate of CaOx aggregation assay and dissolution of calcium oxalate (CaOx) crystal by using titrimetry method. Cystone was used as positive control. The effects of cystone on slope of nucleation and aggregation as well as growth of CaOx were evaluated spectrophotometrically. The highest yield percentage of P.niruri was occupied by methanol (5.74 %). The maximum inhibition against aggregation of CaOx crystals was also occupied by methanol (66.67 % ± 1.61) and was comprised with alkaloid, steroid, terpenoid and tannin. Dissolution effect on calcium oxalate crystals indicates that the aqueous extracts of P. niruri was found to be more effective in dissolution of CaOx with 63.33 % ± 1.44. P. niruri significantly (P < 0.05) inhibited the slope of nucleation and aggregation of CaOx crystallization, and reduced the crystal density. The results of the present study confirmed that P. niruri leaves can be used as remedial mediator for urolithiasis. However, further studies are required for isolation and identification of active constituents and their in-vivo confirmation. 2021 Article PeerReviewed text en http://eprints.uthm.edu.my/1877/1/J12190_a9f73c6a96707e307554d0ecb28648d5.pdf Gul, Muhammad Tayyab and Muhammad, Norhayati and Pauzi, Aslia Natasha and Abu Bakar, Mohd Fadzelly and A. Talip, Balkis and Abdullah, Norazlin and Abdul Rahim, Nur Fazira and Syukriah, Wan Nur Ain and Marzuki, Wan and B. Din, Laily and Ibrahime, Nazlina (2021) Evaluation of Phyllanthus niruri L. from Malaysia for In-vitro Anti-Urolithiatic properties by different solvent extraction. Pakistan Journal of Scientific and Industrial Research Series B: Biological Sciences, 64 (B (1)). pp. 81-86. https://doi.org/10.52763/PJSIR.BIOL.SCI.64.1.2021.81.86 |
| spellingShingle | TP155-156 Chemical engineering Gul, Muhammad Tayyab Muhammad, Norhayati Pauzi, Aslia Natasha Abu Bakar, Mohd Fadzelly A. Talip, Balkis Abdullah, Norazlin Abdul Rahim, Nur Fazira Syukriah, Wan Nur Ain Marzuki, Wan B. Din, Laily Ibrahime, Nazlina Evaluation of Phyllanthus niruri L. from Malaysia for In-vitro Anti-Urolithiatic properties by different solvent extraction |
| title | Evaluation of Phyllanthus niruri L. from Malaysia for In-vitro
Anti-Urolithiatic properties by different solvent extraction |
| title_full | Evaluation of Phyllanthus niruri L. from Malaysia for In-vitro
Anti-Urolithiatic properties by different solvent extraction |
| title_fullStr | Evaluation of Phyllanthus niruri L. from Malaysia for In-vitro
Anti-Urolithiatic properties by different solvent extraction |
| title_full_unstemmed | Evaluation of Phyllanthus niruri L. from Malaysia for In-vitro
Anti-Urolithiatic properties by different solvent extraction |
| title_short | Evaluation of Phyllanthus niruri L. from Malaysia for In-vitro
Anti-Urolithiatic properties by different solvent extraction |
| title_sort | evaluation of phyllanthus niruri l. from malaysia for in-vitro
anti-urolithiatic properties by different solvent extraction |
| topic | TP155-156 Chemical engineering |
| url | http://eprints.uthm.edu.my/1877/ http://eprints.uthm.edu.my/1877/ http://eprints.uthm.edu.my/1877/1/J12190_a9f73c6a96707e307554d0ecb28648d5.pdf |