Isolation of bacteria associated with the tentacles of holothuria (lessonothuria) pardalis (selenka 1867) from Pulau Tinggi, Johor including pigment-producing chryseobacterium sp.

Pigment-producing microorganisms have become a main focus due to the search of food-grade microbial pigments for natural food colourant industry. Therefore, this study aimed to isolate and genetically identify bacteria including pigmentproducing bacterial strains associated with the tentacles of...

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Main Authors:	Solehin, Siti Najihah, Ab Rahman, Nor Shahida, Abu Bakar, Muhammad Abdul Latiff, Mohamed Rehan, Aisyah, Kamarudin, Kamarul Rahim
Format:	Article
Language:	English
Published:	2020
Subjects:	QR Microbiology
Online Access:	http://eprints.uthm.edu.my/1018/ http://eprints.uthm.edu.my/1018/1/J11915_479333f461944695109775bd3690e7c7.pdf

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author	Solehin, Siti Najihah Ab Rahman, Nor Shahida Abu Bakar, Muhammad Abdul Latiff Mohamed Rehan, Aisyah Kamarudin, Kamarul Rahim
author_facet	Solehin, Siti Najihah Ab Rahman, Nor Shahida Abu Bakar, Muhammad Abdul Latiff Mohamed Rehan, Aisyah Kamarudin, Kamarul Rahim
author_sort	Solehin, Siti Najihah
building	UTHM Institutional Repository
collection	Online Access
description	Pigment-producing microorganisms have become a main focus due to the search of food-grade microbial pigments for natural food colourant industry. Therefore, this study aimed to isolate and genetically identify bacteria including pigmentproducing bacterial strains associated with the tentacles of Holothuria (Lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor. Morphological characteristics of bacteria based on Gram staining were observed using a digital compound microscope. Partial 16S ribosomal RNA (rRNA) gene sequencing was also incorporated to support the morphological approach. A number of six partial gene sequences of non-protein coding 16S rRNA were obtained and the Basic Local Alignment Search Tool (BLAST) analysis suggested the presence of three known bacteria species from the genera of Pseudomonas, Chryseobacterium and Klebsiella. The neighbourhood-joining method and Maximum Likelihood method used in the phylogenetic analysis supported the identification and classification of the bacteria species. Among the bacterial isolates, only strain B14 identified as Chryseobacterium sp. produced pigment i.e. orange pigment. The highest point of cell density of Chryseobacterium sp. was recorded at 16 hours. However, further analysis is needed to confirm the pigment characterisation and the potential of this pigment to be exploited as natural colourant in the food industry.
first_indexed	2025-11-15T19:52:45Z
format	Article
id	uthm-1018
institution	Universiti Tun Hussein Onn Malaysia
institution_category	Local University
language	English
last_indexed	2025-11-15T19:52:45Z
publishDate	2020
recordtype	eprints
repository_type	Digital Repository
spelling	uthm-10182021-10-17T07:31:10Z http://eprints.uthm.edu.my/1018/ Isolation of bacteria associated with the tentacles of holothuria (lessonothuria) pardalis (selenka 1867) from Pulau Tinggi, Johor including pigment-producing chryseobacterium sp. Solehin, Siti Najihah Ab Rahman, Nor Shahida Abu Bakar, Muhammad Abdul Latiff Mohamed Rehan, Aisyah Kamarudin, Kamarul Rahim QR Microbiology Pigment-producing microorganisms have become a main focus due to the search of food-grade microbial pigments for natural food colourant industry. Therefore, this study aimed to isolate and genetically identify bacteria including pigmentproducing bacterial strains associated with the tentacles of Holothuria (Lessonothuria) pardalis (Selenka 1867) from Pulau Tinggi, Johor. Morphological characteristics of bacteria based on Gram staining were observed using a digital compound microscope. Partial 16S ribosomal RNA (rRNA) gene sequencing was also incorporated to support the morphological approach. A number of six partial gene sequences of non-protein coding 16S rRNA were obtained and the Basic Local Alignment Search Tool (BLAST) analysis suggested the presence of three known bacteria species from the genera of Pseudomonas, Chryseobacterium and Klebsiella. The neighbourhood-joining method and Maximum Likelihood method used in the phylogenetic analysis supported the identification and classification of the bacteria species. Among the bacterial isolates, only strain B14 identified as Chryseobacterium sp. produced pigment i.e. orange pigment. The highest point of cell density of Chryseobacterium sp. was recorded at 16 hours. However, further analysis is needed to confirm the pigment characterisation and the potential of this pigment to be exploited as natural colourant in the food industry. 2020 Article PeerReviewed text en http://eprints.uthm.edu.my/1018/1/J11915_479333f461944695109775bd3690e7c7.pdf Solehin, Siti Najihah and Ab Rahman, Nor Shahida and Abu Bakar, Muhammad Abdul Latiff and Mohamed Rehan, Aisyah and Kamarudin, Kamarul Rahim (2020) Isolation of bacteria associated with the tentacles of holothuria (lessonothuria) pardalis (selenka 1867) from Pulau Tinggi, Johor including pigment-producing chryseobacterium sp. Journal of Engineering and Health Sciences, 4. pp. 111-122.
spellingShingle	QR Microbiology Solehin, Siti Najihah Ab Rahman, Nor Shahida Abu Bakar, Muhammad Abdul Latiff Mohamed Rehan, Aisyah Kamarudin, Kamarul Rahim Isolation of bacteria associated with the tentacles of holothuria (lessonothuria) pardalis (selenka 1867) from Pulau Tinggi, Johor including pigment-producing chryseobacterium sp.
title	Isolation of bacteria associated with the tentacles of holothuria (lessonothuria) pardalis (selenka 1867) from Pulau Tinggi, Johor including pigment-producing chryseobacterium sp.
title_full	Isolation of bacteria associated with the tentacles of holothuria (lessonothuria) pardalis (selenka 1867) from Pulau Tinggi, Johor including pigment-producing chryseobacterium sp.
title_fullStr	Isolation of bacteria associated with the tentacles of holothuria (lessonothuria) pardalis (selenka 1867) from Pulau Tinggi, Johor including pigment-producing chryseobacterium sp.
title_full_unstemmed	Isolation of bacteria associated with the tentacles of holothuria (lessonothuria) pardalis (selenka 1867) from Pulau Tinggi, Johor including pigment-producing chryseobacterium sp.
title_short	Isolation of bacteria associated with the tentacles of holothuria (lessonothuria) pardalis (selenka 1867) from Pulau Tinggi, Johor including pigment-producing chryseobacterium sp.
title_sort	isolation of bacteria associated with the tentacles of holothuria (lessonothuria) pardalis (selenka 1867) from pulau tinggi, johor including pigment-producing chryseobacterium sp.
topic	QR Microbiology
url	http://eprints.uthm.edu.my/1018/ http://eprints.uthm.edu.my/1018/1/J11915_479333f461944695109775bd3690e7c7.pdf

Isolation of bacteria associated with the tentacles of holothuria (lessonothuria) pardalis (selenka 1867) from Pulau Tinggi, Johor including pigment-producing chryseobacterium sp.

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