Growth And Biochemical Composition Of Isochrysis Maritima Billard And Gayral 1972 Under Photoautotrophic And Heterotrophic Conditions : Assessment For Aquaculture Feed
The effects of photoautotrophic and heterotrophic conditions on growth and biochemical composition of isochrysis maritima, isolated from teluk aling, penang were investigated. Under photoautotrophic condition, i. Maritima was cultivated in different nitrogen sources (nitrate, nitrite, and urea) a...
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| Format: | Thesis |
| Language: | English |
| Published: |
2016
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| Online Access: | http://eprints.usm.my/63090/ http://eprints.usm.my/63090/1/Pages%20from%2017-00001802933.pdf |
| Summary: | The effects of photoautotrophic and heterotrophic conditions on growth and
biochemical composition of isochrysis maritima, isolated from teluk aling, penang
were investigated. Under photoautotrophic condition, i. Maritima was cultivated in
different nitrogen sources (nitrate, nitrite, and urea) and harvested at different growth
phases (exponential, early stationary and late stationary) to examine the impact of
nitrogen sources and growth phases on growth and biochemical composition. The results
revealed that urea at late stationary was the most suitable nitrogen source, which
recorded high cell growth (10.9 ± 0.63 x 106 cell.Ml'1) and biochemical composition
including protein (32.1 ± 3.17%), carbohydrate (37.0 ± 0.36%) and fatty acids content
(total sfa = 32.92 ± 2.59 %; total mufa = 6.26 ± 0.83%; total pufa = 15.55 ± 1.49%)
and was further investigated onto higher volume of walne’s medium (50 l). Under
heterotrophic condition, i. Maritima was cultivated in different organic carbon sources
(glucose, sucrose, lactose, fructose and sodium acetate) at different concentrations (0.02
m, 0.05 m and 1.0 m) with urea as nitrogen source to determine the suitable carbon
sources and concentrations to maximize the growth of this strain. |
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