Isolation And Physicochemical Properties Evaluation Of Pepsin-Solubilised Collagen And Its Hydrolysate From The Integument Of Sea Cucumber (Stichopus Vastus)
Sea cucumber (stichopus vastus) is considered as an underutilised source, since only its stomach and intestines are eaten raw as salad in few asian countries. All other parts including the integument (high content of collagen) are discarded and hence, a valuable by-product having potential food,...
| Main Author: | |
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| Format: | Thesis |
| Language: | English |
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2014
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| Subjects: | |
| Online Access: | http://eprints.usm.my/63061/ http://eprints.usm.my/63061/1/Pages%20from%205-00001780555.pdf |
| _version_ | 1848885147051491328 |
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| author | Abedin, Md. Zainul |
| author_facet | Abedin, Md. Zainul |
| author_sort | Abedin, Md. Zainul |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Sea cucumber (stichopus vastus) is considered as an underutilised source,
since only its stomach and intestines are eaten raw as salad in few asian countries.
All other parts including the integument (high content of collagen) are discarded and
hence, a valuable by-product having potential food, cosmetic and pharmaceutical
applications is wasted. In view of this, the present study was designed to isolate
pepsin-solubilised collagen (psc) from the integument of 5. Vastus and to prepare its
hydrolysate through trypsin hydrolysis for their potential applications. Sds-page
analysis showed that the isolated psc was of type i and consisted of three ai chains
with a yield of 21.3%. Fourier transform infrared (ftir) spectroscopy revealed that
the structure of triple helix was well preserved in the psc. Amino acid composition
showed that glycine was dominant in the psc. Peptide map of the psc digested by
v8 protease was different from that of calf skin type i collagen. |
| first_indexed | 2025-11-15T19:17:58Z |
| format | Thesis |
| id | usm-63061 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:17:58Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-630612025-10-24T07:18:37Z http://eprints.usm.my/63061/ Isolation And Physicochemical Properties Evaluation Of Pepsin-Solubilised Collagen And Its Hydrolysate From The Integument Of Sea Cucumber (Stichopus Vastus) Abedin, Md. Zainul T1-995 Technology(General) Sea cucumber (stichopus vastus) is considered as an underutilised source, since only its stomach and intestines are eaten raw as salad in few asian countries. All other parts including the integument (high content of collagen) are discarded and hence, a valuable by-product having potential food, cosmetic and pharmaceutical applications is wasted. In view of this, the present study was designed to isolate pepsin-solubilised collagen (psc) from the integument of 5. Vastus and to prepare its hydrolysate through trypsin hydrolysis for their potential applications. Sds-page analysis showed that the isolated psc was of type i and consisted of three ai chains with a yield of 21.3%. Fourier transform infrared (ftir) spectroscopy revealed that the structure of triple helix was well preserved in the psc. Amino acid composition showed that glycine was dominant in the psc. Peptide map of the psc digested by v8 protease was different from that of calf skin type i collagen. 2014-04 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/63061/1/Pages%20from%205-00001780555.pdf Abedin, Md. Zainul (2014) Isolation And Physicochemical Properties Evaluation Of Pepsin-Solubilised Collagen And Its Hydrolysate From The Integument Of Sea Cucumber (Stichopus Vastus). PhD thesis, Perpustakaan Hamzah Sendut. |
| spellingShingle | T1-995 Technology(General) Abedin, Md. Zainul Isolation And Physicochemical Properties Evaluation Of Pepsin-Solubilised Collagen And Its Hydrolysate From The Integument Of Sea Cucumber (Stichopus Vastus) |
| title | Isolation And Physicochemical Properties Evaluation Of Pepsin-Solubilised Collagen And Its Hydrolysate From The Integument Of Sea Cucumber (Stichopus Vastus) |
| title_full | Isolation And Physicochemical Properties Evaluation Of Pepsin-Solubilised Collagen And Its Hydrolysate From The Integument Of Sea Cucumber (Stichopus Vastus) |
| title_fullStr | Isolation And Physicochemical Properties Evaluation Of Pepsin-Solubilised Collagen And Its Hydrolysate From The Integument Of Sea Cucumber (Stichopus Vastus) |
| title_full_unstemmed | Isolation And Physicochemical Properties Evaluation Of Pepsin-Solubilised Collagen And Its Hydrolysate From The Integument Of Sea Cucumber (Stichopus Vastus) |
| title_short | Isolation And Physicochemical Properties Evaluation Of Pepsin-Solubilised Collagen And Its Hydrolysate From The Integument Of Sea Cucumber (Stichopus Vastus) |
| title_sort | isolation and physicochemical properties evaluation of pepsin-solubilised collagen and its hydrolysate from the integument of sea cucumber (stichopus vastus) |
| topic | T1-995 Technology(General) |
| url | http://eprints.usm.my/63061/ http://eprints.usm.my/63061/1/Pages%20from%205-00001780555.pdf |