Isolation and identification of pathogenic leptospira spp from environmental samples during and after 2017 flood in Kelantan
Leptospirosis is a zoonosis found worldwide, the main reservoir is the rat. Human infection generally results from exposure to contaminated river or lake or animals. The outbreak of leptospirosis around the world has been associated with heavy rainfall and flooding. These extreme weather events i...
| Main Author: | |
|---|---|
| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2017
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/62895/ http://eprints.usm.my/62895/1/MOHAMMAD%20SALIHEN%20BIN%20KATMAN-E.pdf |
| _version_ | 1848885112778784768 |
|---|---|
| author | Katman, Mohammad Salihen |
| author_facet | Katman, Mohammad Salihen |
| author_sort | Katman, Mohammad Salihen |
| building | USM Institutional Repository |
| collection | Online Access |
| description | Leptospirosis is a zoonosis found worldwide, the main reservoir is the rat. Human infection
generally results from exposure to contaminated river or lake or animals. The outbreak of
leptospirosis around the world has been associated with heavy rainfall and flooding. These
extreme weather events increase the risk of leptospirosis to those who live within
Leptospira-contaminated environments and also the magnitude of outbreaks. The study
aimed to isolate and identify the presence of pathogenic Leptospira spp in an environmental
sample during the flood (52 samples) and after the flood (52 samples) from three districts
which were Tumpat, Pasir Mas and Kota Bharu. The sample was cultured and enriched in
liquid EMJH, a selective media for Leptospira followed by dark-field microscope (DFM)
observation to determine the positive morphology of Leptospira. PCR targeting LipL32
were performed, which is present only in pathogenic Leptospira spp. Findings revealed 35
positive samples (67 %) during the flood for Leptospira under DFM: 19 from Tumpat, 10
from Pasir Mas and 6 from Kota Bharu. Four samples: 3 from Tumpat and 1 from Pasir
Mas were detected as pathogenic Leptospira. Meanwhile, an amount of 32 samples (62 %)
after the flood was found as positive for Leptospira under DFM: 15 from Tumpat, 6 from
Pasir Mas and 11 from Kota Bharu. Eighth samples: 4 from Tumpat, 3 from Pasir Mas and
1 from Kota Bharu were detected as pathogenic Leptospira. Therefore, this study
successfully isolated and identified pathogenic Leptospira spp in environmental samples
affected by flood. |
| first_indexed | 2025-11-15T19:17:25Z |
| format | Monograph |
| id | usm-62895 |
| institution | Universiti Sains Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T19:17:25Z |
| publishDate | 2017 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | usm-628952025-10-09T03:50:41Z http://eprints.usm.my/62895/ Isolation and identification of pathogenic leptospira spp from environmental samples during and after 2017 flood in Kelantan Katman, Mohammad Salihen R Medicine RA643-645 Disease (Communicable and noninfectious) and public health Leptospirosis is a zoonosis found worldwide, the main reservoir is the rat. Human infection generally results from exposure to contaminated river or lake or animals. The outbreak of leptospirosis around the world has been associated with heavy rainfall and flooding. These extreme weather events increase the risk of leptospirosis to those who live within Leptospira-contaminated environments and also the magnitude of outbreaks. The study aimed to isolate and identify the presence of pathogenic Leptospira spp in an environmental sample during the flood (52 samples) and after the flood (52 samples) from three districts which were Tumpat, Pasir Mas and Kota Bharu. The sample was cultured and enriched in liquid EMJH, a selective media for Leptospira followed by dark-field microscope (DFM) observation to determine the positive morphology of Leptospira. PCR targeting LipL32 were performed, which is present only in pathogenic Leptospira spp. Findings revealed 35 positive samples (67 %) during the flood for Leptospira under DFM: 19 from Tumpat, 10 from Pasir Mas and 6 from Kota Bharu. Four samples: 3 from Tumpat and 1 from Pasir Mas were detected as pathogenic Leptospira. Meanwhile, an amount of 32 samples (62 %) after the flood was found as positive for Leptospira under DFM: 15 from Tumpat, 6 from Pasir Mas and 11 from Kota Bharu. Eighth samples: 4 from Tumpat, 3 from Pasir Mas and 1 from Kota Bharu were detected as pathogenic Leptospira. Therefore, this study successfully isolated and identified pathogenic Leptospira spp in environmental samples affected by flood. Universiti Sains Malaysia 2017-05 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/62895/1/MOHAMMAD%20SALIHEN%20BIN%20KATMAN-E.pdf Katman, Mohammad Salihen (2017) Isolation and identification of pathogenic leptospira spp from environmental samples during and after 2017 flood in Kelantan. Project Report. Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R Medicine RA643-645 Disease (Communicable and noninfectious) and public health Katman, Mohammad Salihen Isolation and identification of pathogenic leptospira spp from environmental samples during and after 2017 flood in Kelantan |
| title | Isolation and identification of pathogenic leptospira
spp from environmental samples during and after
2017 flood in Kelantan |
| title_full | Isolation and identification of pathogenic leptospira
spp from environmental samples during and after
2017 flood in Kelantan |
| title_fullStr | Isolation and identification of pathogenic leptospira
spp from environmental samples during and after
2017 flood in Kelantan |
| title_full_unstemmed | Isolation and identification of pathogenic leptospira
spp from environmental samples during and after
2017 flood in Kelantan |
| title_short | Isolation and identification of pathogenic leptospira
spp from environmental samples during and after
2017 flood in Kelantan |
| title_sort | isolation and identification of pathogenic leptospira
spp from environmental samples during and after
2017 flood in kelantan |
| topic | R Medicine RA643-645 Disease (Communicable and noninfectious) and public health |
| url | http://eprints.usm.my/62895/ http://eprints.usm.my/62895/1/MOHAMMAD%20SALIHEN%20BIN%20KATMAN-E.pdf |