A comparative study on the antioxidant activity of leaf, flower, stem and root extracts of peperomia pellucida (l.) hbk (piperaceae)
Peperomia pellucida is an underexploited annual herb abundantly available in Malaysia. It is widely used as ethnomedicines in treating various ailments throughout the tropical and subtropical regions of the world. Previous studies evidenced that P. pellucida possessed antioxidant properties. Howe...
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| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2016
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| Online Access: | http://eprints.usm.my/62722/ http://eprints.usm.my/62722/1/MOK%20SIEW%20FANG%20-%20e.pdf |
| Summary: | Peperomia pellucida is an underexploited annual herb abundantly available in
Malaysia. It is widely used as ethnomedicines in treating various ailments throughout
the tropical and subtropical regions of the world. Previous studies evidenced that P.
pellucida possessed antioxidant properties. However, there is no antioxidant study on
different parts of the plant was reported. The aim of this study is to determine and
compare the antioxidant properties of leaf, flower, stem, and root of P. pellucida, hence
providing additional scientific evidence of its potential. Each part of the plant collected was washed, freeze-dried, and ground into fine powder before extracted using 70% ethanol, 70% methanol and distilled water (1:10 w/v). DPPH (2,2-diphenyl-lpicrylhydrazyl)
radical scavenging assay was used to determine the antioxidant capacity.
Only moderate [half maximal inhibitory concentration (IC50) >0.05 mg/ml] antioxidant
activities were detected in all P. pellucida extracts with mean IC50 ranging from 2.14
mg/ml (ethanol flower extract) to 10.06 mg/ml (methanol stem extract). The mean IC50
of all P. pellucida extracts is significantly different from that of standard reference,
gallic acid (0.03 mg/ml) (p<0.05). Generally, P. pellucida leaf extracts showed the
highest antioxidant activity, followed by flower, root, and stem extracts. However,
ethanolic flower extract (IC50 2.14 mg/ml) showed significantly higher antioxidant
activity than ethanolic leaf extract (IC50 2.79 mg/ml) (p<0.05). In terms of extraction
solvent, ethanolic extracts of P. pellucida generally showed higher antioxidant activity
than that of methanolic and aqueous extracts. However, methanolic root extract (IC50 4.35 mg/ml) showed significantly higher antioxidant activity than ethanolic root extract
(IC50 6.15 mg/ml) (p<0.05). The results revealed that ethanol is a better extraction
solvent than methanol and distilled water for leaf, flower and stem of P. pellucida to
show their highest antioxidant activity while methanol is a better extraction solvent for
P. pellucida root to show its highest antioxidant activity on DPPH assay. |
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